货号：70-650

品牌：Merck Millipore

规格：

目录价：￥11220.00

市场价格：￥9537.00

会员价格：￥8976.00

金山科研平台，产品价格货期咨询微信：jinshanbio Description: AP-1 Family EZ-TFA Transcription Factor Assay Chemiluminescent View All» Trade Name: Upstate (Millipore) View All» Qty/Pk: 192 assays View All» Product Overview: The Non-Radioactive Jun/c-Fos Transcription Factor Assay kit is provided in a 96-well format. During the assay, the Capture Probe, a double stranded biotinylated oligonucleotide containing the flanked DNA binding consensus sequence for c-fos (5'- TGA(C/G)TCA -3'), is mixed with cellular (nuclear) extract in the Transcription Factor Assay Buffer provided. When incubated together, the active form of Jun and Fos contained in the nuclear extract binds to the consensus sequence. The extract/probe/buffer mixture is then directly transferred to the streptavidin coated plate. The active Jun/Fos transcription factor proteins is immobilized on the biotinylated double stranded oligonucleotide capture probe bound to the streptavidin plate well, and any unbound material is washed away. The bound c-Jun/c-Fos transcription factors are detected with specific primary antibodies, a mouse monoclonal anti-c-Jun and a rabbit anti-c-Fos. A highly sensitive HRP-conjugated secondary antibody is then used for detection. This provides sensitive chemiluminescent detection that can be read in a microplate luminometer or by a CCD camera-coupled imaging system. Included in the kit are positive cell extract, a non-specific double stranded oligonucleotide, and a specific competitor double stranded oligonucleotide. View All» Key Applications: Activity Assay View All» Species Reactivity:

• Human

• Mouse

• Rat
  View All» Usage Statement: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. View All» Entrez Gene Summary: This gene is the putative transforming gene of avian sarcoma virus 17. It encodes a protein which is highly similar to the viral protein, and which interacts directly with specific target DNA sequences to regulate gene expression. This gene is intronless and is mapped to 1p32-p31, a chromosomal region involved in both translocations and deletions in human malignancies. View All» UniProt Summary: FUNCTION: SwissProt: P05412 # Transcription factor that recognizes and binds to the enhancer heptamer motif 5'-TGA[CG]TCA-3'.SIZE: 331 amino acids; 35676 Da SUBUNIT: Interacts with FOS to form a dimer. Interacts with SMAD3/SMAD4 heterodimers. Interacts with MYBBP1A, SPIB and TCF20. Interacts with COPS5; indirectly leading to its phosphorylation. Interacts with DSIPI; this interaction inhibits the binding of active AP1 to its target DNA.SUBCELLULAR LOCATION: Nucleus.PTM: Phosphorylation enhances the transcriptional activity. Phosphorylated by PRKDC.SIMILARITY: SwissProt: P05412 ## Belongs to the bZIP family. Jun subfamily. & Contains 1 bZIP domain. View All» Brand Family: Upstate View All» Storage Conditions: The Transcription Factor Assay is a triple temperature storage assay kit. · Store kit components at the temperatures indicated on the labels and this insert until their expiration date. · Store HeLa (4hr serum Response) or WI-38 Nuclear Extracts at -80°C. Avoid repeated freeze-thaw cycles. Stable at -80°C for 6 months. · Store the AP-1 Capture Probe, Competitor Oligonucleotides, Negative Control Probe, and all antibodies at -20°C. · Store the Transcription Factor Assay (TFA) Buffer at -20°C. Avoid repeated freeze-thawing of the TFA Buffer. Buffer can be quickly thawed in warm water prior to use. Buffer can be aliquotted and frozen. · Store the TFA 96-well plate and the TMB/E and stop solutions at 4°C. View All» Gene Symbol:
  • JUN

  • AP1

  • p39

  • c-Jun
    View All» Product Name: AP-1 Family EZ-TFA Transcription Factor Assay Chemiluminescent View All» Materials Required but Not Delivered: 1. Nuclear Extraction Kit (Catalog No. 2900) or suitable extraction reagents 2. Pipettor (both single and multi-channel) 3. Pipette tips 4. Distilled water 5. Microplate reader (wavelength 450nm/650nm) (650nm optional) 6. Rotator or Plate shaker (optional) 7. Plate cover (optional) View All» Components:
    • Rabbit anti-c-Fos Primary Antibody (Part No. 2006131): One vial containing 15 μL Rabbit anti-c-Fos. Use at 1:500 dilution in Enhanced Transcription Factor Assay Buffer

    • Rabbit anti-FosB Primary Antibody (Part No. 2006132): One vial containing 15 μL of anti-FosB. Use at 1:500 dilution in Enhanced Transcription Factor Assay Buffer

    • Rabbit anti-Fra-1 Primary Antibody (Part No. 2006127): One vial containing 10 μL of anti-Fra-1. Use at 1:1000 dilution in Enhanced Transcription Factor Assay Buffer

    • Rabbit anti-Fra-2 Primary Antibody (Part No. 2006128): One vial containing 10 μL of anti-Fra-1. Use at 1:1000 dilution in Enhanced Transcription Factor Assay Buffer

    • Mouse anti-c-Jun Primary Antibody (Part No. 2006130): One vial containing 10 μL of anti-c-jun. Use at 1:1000 dilution in Enhanced Transcription Factor Assay Buffer

    • Rabbit anti-JunB Primary Antibody (Part No. 2006126): One vial containing 15 μL of anti-c-jun. Use at 1:500 dilution in Enhanced Transcription Factor Assay Buffer

    • Rabbit anti-JunD Primary Antibody (Part No. 2006129): One vial containing 15 μL of anti-c-jun. Use at 1:500 dilution in Enhanced Transcription Factor Assay Buffer

    • AP-1 Capture Probe (Part No. 2006124): One vial containing 440 μL biotinylated double stranded oligonucleotide probe (1 pmol/μL) containing the wild-type TRE consensus sequence (5'-TGA(C/G)TCA-3').

    • TFA Negative Control Probe (Part No. 2003486): Two vials containing 40 μL biotinylated double stranded oligonucleotide probe (2 pmol/μL). Provided as an internal negative control, this non-specific competitor oligonucleotide does not contain the NFκB consensus sequence.

    • AP-1 Specific Competitor Oligonucleotide (Part No. 2006133): One vial containing 40 μL (10 pmol/μL) specific competitor oligonucleotide. The AP-1 Competitor Oligonucleotide has the same consensus sequence (5'-TGA(C/G)TCA-3')

    • WI-38 Nuclear Extract (Part No. 2003764): One vial containing 25 μL WI-38 nuclear extract. This extract is provided as a positive control and is not intended for extract-to-extract, nor plate-to-plate comparisons. When using this control, you will notice a decrease in signal with repeated freeze/thaw cycles. This component must be stored at -80°C.

    • HeLa Nuclear Extract, 4 hr Serum Response (Part No. 90558): One vial containing 25 μL 10% serum treated HeLa nuclear extract. This extract is provided as a positive control and is not intended for extract-to-extract, nor plate-to-plate comparisons. When using this control, you will notice a decrease in signal with repeated freeze/thaw cycles. This component must be stored at -80°C.

    • EZ-TFA (Universal Transcription Factor Assay, Chemiluminescent) Catalog #70-601. One universal kit containing 125 mL TFA Buffer, two streptavidin-coated stripwell plate, 24 grams of blocking reagent, 250 μl 10X annealing buffer, two 12 mL bottles TMB/E, two 12 mL bottles stop solution, 60 μl Gt X Rb HRP secondary antibody, 60 μl Gt X Ms HRP secondary antibody. A detailed manual is also included with the 70-601 Universal TFA component.
      View All» UniProt Number:
      • P05412

      • P01100
        View All» Packaging: 96 wells per plate View All» Entrez Gene Number: NM_002228.3 View All»
        © 金山科研平台是专业的授权总代理区域代理经销平台。
        © 如需询价，请加客服QQ：1749072012 、客服微信：jinshanbio，或发送邮件到1749072012@qq.com
        © 平台为生命科学研究相关领域提供一站式耗材试剂仪器解决方案和采购服务，数据资源基于CC协议。
        © 本文地址：https://www.16ao.com/thread-150860.htm
        © Merck Millipore代理70-650 AP-1 Family EZ-TFA Transcription Factor Assay Chemiluminescent，产品报价联系微信jinshanbio