货号：AB10025

品牌：Merck Millipore

规格：

目录价：￥4354.00

市场价格：￥3700.90

会员价格：￥3483.20

金山科研平台，产品价格货期咨询微信：jinshanbio Description: Anti-Ubiquitin-conjugating enzyme E2 N Antibody | AB10025 View All» Promotional Text: Special Shipping Offer on Antibodies100% Performance Guaranteed View All» Specificity: This antibody recognizes the internal domain of Ubiquitin-conjugating enzyme E2 N. View All» Molecular Weight: ~ 17 kDa View All» Epitope: Internal domain View All» Immunogen: KLH-conjugated linear peptide corresponding to the internal domain of Ubiquitin-conjugating enzyme E2 N. View All» Background Information: Ubiquitin-conjugating enzyme E2 N is a specific E2 enzyme involved in protein degradation. This enzyme is also involved in DNA repair and DNA damage responses and is known for modifying chromation. E2 enzymes possess a core catalytic domain required for ubiquitin conjugation and are typically defined by a catalytic cysteine that forms a thioester bond with ubiquitin. View All» Species Reactivity:

• Human

• Mouse

• Rat

• Canine

• Horse
  View All» Species Reactivity Note: Demonstrated to react with human, mouse, and rat. Predicted to react with canine and equine based on 100% sequence homology. View All» Application Notes: Western Blot (SNAP ID) Analysis: 0.5 µg/mL from a previous lot detected Ubiquitin-conjugating enzyme E2 N on 10 µg of HEPG2 cell lysate.Immunohistochemistry Analysis: 1:300 dilution from a previous lot detected Ubiquitin-conjugating enzyme E2 N in ovarian cancer and renal cell carcinoma tissue. View All» Control: HEPG2 cell lysate View All» Quality Assurance: Evaluated by Western Blot in HEPG2 cell lysate.Western Blot Analysis: 0.1 µg/mL of this antibody detected Ubiquitin-conjugating enzyme E2 N on 10 µg of HEPG2 cell lysate. View All» Purification Method: Affinity Purfied View All» Presentation: Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide. View All» Storage Conditions: Stable for 1 year at 2-8°C from date of receipt. View All» UniProt Number: P61088 View All» Entrez Gene Number: NP_003339 View All» Gene Symbol:
  • UBE2N

  • BLU

  • UBC13

  • Ubc13

  • UbcH-ben
    View All» Alternate Names:
    • Bendless-like ubiquitin-conjugating enzyme

    • Ubiquitin carrier protein N

    • Ubiquitin-protein ligase N

    • bendless-like ubiquitin conjugating enzyme

    • ubiquitin-conjugating enzyme E2N

    • ubiquitin-conjugating enzyme E2N (UBC13 homolog, yeast)

    • ubiquitin-conjugating enzyme E2N (homologous to yeast UBC13)

    • yeast UBC13 homolog
      View All» Usage Statement: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. View All» Key Applications:
      • Western Blotting

      • Immunohistochemistry
        View All» Entrez Gene Summary: The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. Studies in mouse suggest that this protein plays a role in DNA postreplication repair. [provided by RefSeq]. View All» UniProt Summary: FUNCTION: The UBE2V2/UBE2N heterodimer catalyzes the synthesis of non-canonical poly-ubiquitin chains that are linked through 'Lys-63'. This type of poly-ubiquitination does not lead to protein degradation by the proteasome. Mediates transcriptional activation of target genes. Plays a role in the control of progress through the cell cycle and differentiation. Plays a role in the error-free DNA repair pathway and contributes to the survival of cells after DNA damage. Acts together with the E3 ligases, HLTF and SHPRH, in the 'Lys-63'-linked poly-ubiquitination of PCNA upon genotoxic stress, which is required for DNA repair. CATALYTIC ACTIVITY: ATP + ubiquitin + protein lysine = AMP + diphosphate + protein N-ubiquityllysine.PATHWAY: Protein modification; protein ubiquitination.SUBUNIT STRUCTURE: Heterodimer with UBE2V2 by similarity. Interacts with RNF8 and RNF168. Interacts with RNF11. Interacts with the E3 ligases, HLTF and SHPRH; the interactions promote the 'Lys-63'-linked polyubiquitination of PCNA upon genotoxic stress and lead to DNA repair By similarity. PTM: Conjugation to ISG15 impairs formation of the thioester bond with ubiquitin but not interaction with UBE2V2.SEQUENCE SIMILARITIES: Belongs to the ubiquitin-conjugating enzyme family. View All» Product Name: Anti-Ubiquitin-conjugating enzyme E2 N View All» Concentration: 0.5 mg/mL View All» Antibody Type: Polyclonal Antibody View All» Qty/Pk: 100 μg View All» Format: Affinity Purified View All» Host: Rabbit View All»
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