货号：AB10423

品牌：Merck Millipore

规格：400Ul

目录价：￥5666.00

市场价格：￥4816.10

会员价格：￥4532.80

金山科研平台，产品价格货期咨询微信：jinshanbio Description: Anti-Phospho- Bad (Ser75) Antibody | AB10423 View All» Promotional Text: Special Shipping Offer on Antibodies100% Performance Guaranteed View All» Specificity: The antibody reacts with the 18 kDa Phospho-Bad-S75 protein. View All» Molecular Weight: 18 kDa View All» Immunogen: This antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S75 of human Bad. View All» Modifications: Phosphorylation View All» Background Information: Bad is a member of the BCL-2 family. BCL-2 family members are known to be regulators of programmed cell death. This protein positively regulates cell apoptosis by forming heterodimers with BCL-xL and BCL-2, and reversing their death repressor activity. Proapoptotic activity of this protein is regulated through its phosphorylation. Protein kinases AKT and MAP kinase, as well as protein phosphatase calcineurin are found to be involved in the regulation of this protein. Bad is phosphorylated on one or more of Ser-75, Ser-99, Ser-118 and Ser-134 in response to survival stimuli, which blocks its pro-apoptotic activity. Phosphorylation on Ser-99 or Ser-75 promotes heterodimerization with 14-3-3 proteins. This interaction then facilitates the phosphorylation at Ser-118, a site within the BH3 motif, leading to the release of Bcl-X(L) and the promotion of cell survival. Ser-99 is the major site of AKT/PKB phosphorylation, Ser-118 the major site of protein kinase A (CAPK) phosphorylation. View All» Species Reactivity:

• Mouse

• Rat
  View All» Species Reactivity Note: Reacts with mouse and rat. Other species have not been tested. View All» Control: Western Blot : RAW264.7/LPS lysate. View All» Quality Assurance: Western Blot Analysis: 1:250 dilution of this antibody detected Phospho-Bad-S75 on RAW264.7/LPS lysate. View All» Purification Method: Protein G followed by IAP View All» Presentation: Purified in PBS with 0.09% NaN3. View All» Storage Conditions: Store at 2-8°C for 6 months from date of receipt. For long term storage, store at -20°C View All» UniProt Number: Q92934 View All» Entrez Gene Number: NP_116784 View All» Gene Symbol:
  • BBC2

  • BBC6

  • BCL2L8
    View All» Alternate Names:
    • BCL-X/BCL-2 binding protein

    • BCL2-antagonist of cell death protein

    • BCL2-associated agonist of cell death

    • BCL2-binding component 6

    • BCL2-binding protein

    • Bcl-2-binding component 6

    • Bcl-2-like 8 protein

    • Bcl-XL/Bcl-2-associated death promoter
      View All» Usage Statement: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. View All» Key Applications: Western Blotting View All» Entrez Gene Summary: The protein encoded by this gene is a member of the BCL-2 family. BCL-2 family members are known to be regulators of programmed cell death. This protein positively regulates cell apoptosis by forming heterodimers with BCL-xL and BCL-2, and reversing their death repressor activity. Proapoptotic activity of this protein is regulated through its phosphorylation. Protein kinases AKT and MAP kinase, as well as protein phosphatase calcineurin were found to be involved in the regulation of this protein. Alternative splicing of this gene results in two transcript variants which encode the same isoform. [provided by RefSeq] View All» UniProt Summary: FUNCTION: Promotes cell death. Successfully competes for the binding to Bcl-X(L), Bcl-2 and Bcl-W, thereby affecting the level of heterodimerization of these proteins with BAX. Can reverse the death repressor activity of Bcl-X(L), but not that of Bcl-2 By similarity. Appears to act as a link between growth factor receptor signaling and the apoptotic pathways.SUBUNIT STRUCTURE: Forms heterodimers with the anti-apoptotic proteins, Bcl-X(L), Bcl-2 and Bcl-W. Also binds protein S100A10 By similarity. The Ser-75/Ser-99 phosphorylated form binds 14-3-3 proteins By similarity.SUBCELLULAR LOCATION: Mitochondrion outer membrane. Cytoplasm. Note= Upon phosphorylation, locates to the cytoplasm.TISSUE SPECIFICTY: Expressed in a wide variety of tissues.DOMAIN: Intact BH3 motif is required by BIK, BID, BAK, BAD and BAX for their pro-apoptotic activity and for their interaction with anti-apoptotic members of the Bcl-2 family.PTM: Phosphorylated on one or more of Ser-75, Ser-99, Ser-118 and Ser-134 in response to survival stimuli, which blocks its pro-apoptotic activity. Phosphorylation on Ser-99 or Ser-75 promotes heterodimerization with 14-3-3 proteins. This interaction then facilitates the phosphorylation at Ser-118, a site within the BH3 motif, leading to the release of Bcl-X(L) and the promotion of cell survival. Ser-99 is the major site of AKT/PKB phosphorylation, Ser-118 the major site of protein kinase A (CAPK) phosphorylation By similarity.SEQUENCE SIMILARITIES: Belongs to the Bcl-2 family.SEQUENCE CAUTION: The sequence AAB36516.1 differs from that shown. Reason: Frameshift at positions 64 and 91. View All» Product Name: Anti-Phospho- Bad (Ser75) Antibody | AB10423 View All» Concentration: 0.25 mg/ml View All» Antibody Type: Polyclonal Antibody View All» Qty/Pk: 100 μg View All» Format: Purified View All» Host: Rabbit View All»
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