货号：AB1076

品牌：Merck Millipore

规格：100Ug

目录价：￥5359.00

市场价格：￥4555.15

会员价格：￥4287.20

金山科研平台，产品价格货期咨询微信：jinshanbio Description: Anti-phospho-ILK (Ser246) Antibody | AB1076 View All» Promotional Text: Special Shipping Offer on Antibodies100% Performance Guaranteed View All» Specificity: This antibody recognizes ILK when phosphorylated at Ser246. View All» Molecular Weight: ~54 kDa observed View All» Epitope: Phosphorylated Ser246 View All» Immunogen: KLH-conjugated linear peptide corresponding to human ILK phosphorylated at Ser246. View All» Modifications: Phosphorylation View All» Background Information: ILK (Integrin-Linked Kinase) is an intraceullar Ser/Thr kinase with four Ankyrin-like repeats. It is known to play pivotal roles in extracellular matrix mediated signaling. ILK is known to associate with β-Integrins in addition to many other focal adhesion associated proteins. In this context, ILK is involved cell adhesion and migration. The kinase activity appears to be reduced following integrin activation, and overexpression of p59 ILK inhibits cellular adhesion to integrin substrates. Additionally, ILK has been associated with epithelial mesechymal transition (EMT) in a TGF-β1 induced Smad dependent manner (Li, 2003). Forced expression of ILK also induced the expression of MMP-2 (matrix metalloprotease 2) and promoted cell migration and invasion (Li, 2003). View All» Species Reactivity:

• Human

• Mouse

• Rat

• Ox
  View All» Species Reactivity Note: Demonstrated to react with human, mouse, and rat. Predicted to react with ox based on 100% sequence homology. View All» Application Notes: Immunofluorescence Analysis: A previous lot was used by an independent laboratory in BT549 breast cancer cells. (Dr. Shoukat Dedhar, BC Cancer Research Centre, Vancouver, BC, Canada.) View All» Control: HeLa cell lysate View All» Quality Assurance: Evaluated by Western Blot in HeLa cell lysate.Western Blot Analysis: 0.1 µg/mL of this antibody detected ILK in 10 µg of HeLa cell lysate. View All» Purification Method: Affinity Purified View All» Presentation: Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. View All» Storage Conditions: Stable for 1 year at 2-8°C from date of receipt. View All» UniProt Number: Q13418 View All» Entrez Gene Number: NP_004508 View All» Gene Symbol:
  • ILK-1

  • ILK1

  • ILK-2

  • ILK2

  • P59

  • p59ILK
    View All» Alternate Names:
    • integrin-linked kinase

    • 59 kDa serine/threonine-protein kinase
      View All» Usage Statement: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. View All» Key Applications:
      • Western Blotting

      • Immunofluorescence
        View All» Entrez Gene Summary: Transduction of extracellular matrix signals through integrins influences intracellular and extracellular functions, and appears to require interaction of integrin cytoplasmic domains with cellular proteins. Integrin-linked kinase (ILK), interacts with the cytoplasmic domain of beta-1 integrin. This gene encodes a serine/threonine protein kinase with 4 ankyrin-like repeats, which associates with the cytoplasmic domain of beta integrins and acts as a proximal receptor kinase regulating integrin-mediated signal transduction. Multiple alternatively spliced transcript variants encoding the same protein have been found for this gene. [provided by RefSeq]. View All» UniProt Summary: FUNCTION: Receptor-proximal protein kinase regulating integrin-mediated signal transduction. May act as a mediator of inside-out integrin signaling. Focal adhesion protein part of the complex ILK-PINCH. This complex is considered to be one of the convergence points of integrin- and growth factor-signaling pathway. Could be implicated in mediating cell architecture, adhesion to integrin substrates and anchorage-dependent growth in epithelial cells. Phosphorylates beta-1 and beta-3 integrin subunit on serine and threonine residues, but also AKT1 and GSK3B.CATALYTIC ACTIVITY: ATP + a protein = ADP + a phosphoprotein.ENZYME REGULATION: Stimulated rapidly but transiently by both cell fibronectin interactions, as well as by insulin, in a PI3-K-dependent manner, likely via the binding of PtdIns(3,4,5)P3 with a PH-like domain of ILK.SUBUNIT STRUCTURE: Interacts with cytoplasmic domain of beta 1 subunit of integrin. Could also interacts with beta 2, beta 3 and/or beta 5 subunit of integrin. Interacts (via ANK repeats) with LIMS1 and LIMS2. Interacts with parvins and probably TGFB1I1.SUBCELLULAR LOCATION: Cell junction › focal adhesion. Cell membrane; Peripheral membrane protein; Cytoplasmic side Ref.10. TISSUE SPECIFICTY: Highly expressed in heart followed by skeletal muscle, pancreas and kidney. Weakly expressed in placenta, lung and liver.DOMAIN: A PH-like domain is involved in phosphatidylinositol phosphate binding.PTM: Autophosphorylated on serine residues.SEQUENCE SIMILARITIES: Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family.Contains 5 ANK repeats.Contains 1 protein kinase domain.SEQUENCE CAUTION: The sequence CAB94832.1 differs from that shown. Reason: Probable cloning artifact. Was originally thought to be distinct gene (ILK2). View All» Product Name: Anti-phospho-ILK (Ser246) View All» Concentration: 1.0 mg/mL View All» Antibody Type: Polyclonal Antibody View All» Qty/Pk: 100 μg View All» Format: Affinity Purified View All» Host: Rabbit View All»
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