货号：AB1410

品牌：Merck Millipore

规格：EA

目录价：￥3468.00

市场价格：￥2947.80

会员价格：￥2774.40

金山科研平台，产品价格货期咨询微信：jinshanbio Description: Anti-Estrogen Receptor β Antibody, a.a. 1-12 human ER-β | AB1410 View All» Promotional Text: Special Shipping Offer on Antibodies100% Performance Guaranteed View All» Trade Name: Chemicon (Millipore) View All» Specificity: Recognizes Human estrogen receptor-beta; isoform beta1 is expressed in testis and ovary, and at a lower level in heart, brain, placenta, liver, skeletal muscle, spleen, thymus, prostate, colon, bone marrow and other tissues. There are 8 isoforms known for estrogen receptor beta. Sequence used is conserved in many species and all known isoforms. View All» Molecular Weight: 55 - 59 kDa View All» Epitope: a.a. 1-12 human ER-beta View All» Immunogen: 12 amino acid peptide corresponding ot NH2 terminus of human ER-beta. Antigenic site: intracytoplasmic. {MDIKNSPSSLNS} peptide is not available for blocking from Chemicon. View All» Species Reactivity: Human View All» Application Notes: Immunoblotting: Should recognize a band between 55kDa and 59kDa depending on isoform presence. Immunohistochemistry on paraffin-embedded tissue sections using 10% neutral buffered formalin as a fixative. 1:200-1:500 with citrate buffer pH 6.0 antigen retrieval with steam heat 30 minutes. Immunocytochemistry, light fixation (2.5% PFA) Optimal working dilutions must be determined by the end user. View All» Control: Myometrium, Breast, Uterus, Prostate View All» Purification Method: Ammonium sulfate precipitation View All» Presentation: Purified immunoglobulin - IgG fraction. Liquid containing PBS, pH 8.0, and 0.1% sodium azide as a preservative. View All» Storage Conditions: Maintain for 1 year at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. View All» UniProt Number: Q92731 View All» Entrez Gene Number:

• NM_001040275.1

• NM_001040276.1

• NM_001437.2
  View All» Gene Symbol:
  • ESR2

  • ESR-BETA

  • ESRB

  • ER-BETA

  • ER-beta

  • NR3A2

  • ESTRB

  • Erb
    View All» Alternate Names: Erbeta View All» Usage Statement: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. View All» Key Applications:
    • Immunocytochemistry

    • Immunohistochemistry (Paraffin)

    • Western Blotting
      View All» Entrez Gene Summary: This gene encodes a member of the family of estrogen receptors and superfamily of nuclear receptor transcription factors. The gene product contains an N-terminal DNA binding domain and C-terminal ligand binding domain and is localized to the nucleus, cytoplasm, and mitochondria. Upon binding to 17beta-estradiol or related ligands, the encoded protein forms homo- or hetero-dimers that interact with specific DNA sequences to activate transcription. Some isoforms dominantly inhibit the activity of other estrogen receptor family members. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of some of these variants has not been fully characterized. View All» UniProt Summary: FUNCTION: SwissProt: Q92731 # Nuclear hormone receptor. Binds estrogens with an affinity similar to that of ESR1, and activates expression of reporter genes containing estrogen response elements (ERE) in an estrogen-dependent manner. Isoform beta-cx lacks ligand binding ability and has no or only very low ere binding activity resulting in the loss of ligand-dependent transactivation ability. DNA- binding by ESR1 and ESR2 is rapidly lost at 37 degrees Celsius in the absence of ligand while in the presence of 17 beta-estradiol and 4-hydroxy-tamoxifen loss in DNA-binding at elevated temperature is more gradual.SIZE: 530 amino acids; 59216 Da SUBUNIT: Binds DNA as a homodimer. Can form a heterodimer with ESR1. Interacts with NCOA3, NCOA5 and NCOA6 coactivators, leading to a strong increase of transcription of target genes. Interacts with PELP1 and UBE1C. Isoform beta-2/cx preferentially forms a hetereodimer with ESR1 rather than ESR2 and inhibits DNA-binding by ESR1. Interacts with AKAP13.SUBCELLULAR LOCATION: Nucleus.TISSUE SPECIFICITY: Isoform beta-1 is expressed in testis and ovary, and at a lower level in heart, brain, placenta, liver, skeletal muscle, spleen, thymus, prostate, colon, bone marrow, mammary gland and uterus. Also found in uterine bone, breast, and ovarian tumor cell lines, but not in colon and liver tumors. Isoform beta-2 is expressed in spleen, thymus, testis and ovary and at a lower level in skeletal muscle, prostate, colon, small intestine, leukocytes, bone marrow, mammary gland and uterus. Isoform beta-3 is found in testis. Isoform beta-4 is expressed in testis, and at a lower level in spleen, thymus, ovary, mammary gland and uterus. Isoform beta-5 is expressed in testis, placenta, skeletal muscle, spleen and leukocytes, and at a lower level in heart, lung, liver, kidney, pancreas, thymus, prostate, colon, small intestine, bone marrow, mammary gland and uterus. Not expressed in brain.DOMAIN:SwissProt: Q92731 Composed of three domains: a modulating N-terminal domain, a DNA-binding domain and a C-terminal steroid-binding domain.SIMILARITY: Belongs to the nuclear hormone receptor family. NR3 subfamily. & Contains 1 nuclear receptor DNA-binding domain. View All» Brand Family: Chemicon View All» Product Name: Anti-Estrogen Receptor β Antibody, a.a. 1-12 human ER-β | AB1410 View All» Concentration: 1 mg/mL View All» Antibody Type: Polyclonal Antibody View All» Qty/Pk: 100 μg View All» Format: Purified View All» Host: Rabbit View All»
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