货号：AB143

品牌：Merck Millipore

规格：50UL

目录价：￥5820.00

市场价格：￥4947.00

会员价格：￥4656.00

金山科研平台，产品价格货期咨询微信：jinshanbio Description: Anti-Choline Acetyltransferase (ChAT) Antibody | AB143 View All» Promotional Text: Special Shipping Offer on Antibodies100% Performance Guaranteed View All» Trade Name: Chemicon (Millipore) View All» Specificity: Cholinergic neurons in brain and central nervous systems. View All» Immunogen: Human placental enzyme. View All» Background Information: Acetylcholine (ACh) is a common neurotransmitter for motoneurons, preganglionic autonomic neurons, postganglionic parasympathetic neurons, a variety of brain regions and some emerging neuron-like stem cells. The metabolism of Ach is relatively simple, involving only two enzymes: choline acetyltransferase (ChAT) for synthesis and acetylcholinesterase (AChE) for degradation. Further, acetylcholine has little function in neurons other than neurotransmission and seems to be neuron specific. It seems that only cholinergic neurons have significant amounts of ChAT making anti-cholinacetyltransferase a useful specific marker. View All» Species Reactivity:

• Human

• Rat

• Mouse

• Bat

• Feline

• Monkey
  View All» Species Reactivity Note: Human, Mouse, Rat, Monkey, Bat, Feline. Reactivity with other species has not been confirmed with IHC. View All» Application Notes: Immunoprecipitation: 1:200 dilution of a previous lot immunoprecipitated 68 kDa active ChAT from brain and placenta. Also precipitates a 27 kDa protein that is co-expressed with ChAT (but lacking ChAT activity) in some cholinergic neurons of the central nervous system of Humans and possibly other mammals.Optimal working dilutions and protocls must be determined by end user. View All» Control: Cerebral cortex tissue. View All» Quality Assurance: Routinely evaluated by immunohistochemistry (paraffin) on normal cerebral cortex tissue.Immunohistochemistry (Paraffin) Analysis:ChAT (cat. # AB143) staining on normal cerebral cortex. Tissue treated with no antigen retrieval. The antibody was diluted to 1:100, using IHC-Select® Detection with HRP-DAB. Immunoreactivity is seeing as a staining pattern on the cell body, dendrites and axons of neurons (brown). View All» Purification Method: Unpurified View All» Presentation: Rabbit polyclonal antisera, liquid, no preservatives. View All» Storage Conditions: Stable for 1 year at -20°C in undiluted aliquots from date of receipt. Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. View All» UniProt Number: P28329 View All» Entrez Gene Number: NP_065574 View All» Gene Symbol:
  • CHAT

  • ChAT

  • CHOACTase

  • CMS1A

  • CMS1A2
    View All» Alternate Names:
    • Choline acetylase

    • acetyl CoA:choline O-acetyltransferase

    • choline acetyltransferase
      View All» Usage Statement: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. View All» Key Applications:
      • Immunohistochemistry

      • Immunohistochemistry (Paraffin)

      • Immunoprecipitation

      • Western Blotting
        View All» Entrez Gene Summary: This gene encodes an enzyme which catalyzes the biosynthesis of the neurotransmitter acetylcholine. This gene product is a characteristic feature of cholinergic neurons, and changes in these neurons may explain some of the symptoms of Alzheimer disease. Mutations in this gene are associated with congenital myasthenic syndrome associated with episodic apnea. Multiple transcript variants encoding different isoforms have been found for this gene, and some of these variants have been shown to encode more than one isoform. [provided by RefSeq]. Transcript Variant: This variant (M) contains alternate 5' exon M, uses an upstream start codon, and encodes isoform 2. View All» UniProt Summary: FUNCTION: SwissProt: P28329 # Catalyzes the reversible synthesis of acetylcholine (ACh) from acetyl CoA and choline at cholinergic synapses.SIZE: 748 amino acids; 82568 Da DISEASE: SwissProt: P28329 # Defects in CHAT are the cause of familial infantile myasthenia gravis 2 (FIMG2) [MIM:254210, 254200]; also known as CMS-EA. FIMG2 patients have myasthenic symptoms since birth or early infancy, negative tests for anti-AChR antibodies, and abrupt episodic crises with increased weakness, bulbar paralysis, and apnea precipitated by undue exertion, fever, or excitement. Inheritance is autosomal recessive.SIMILARITY: SwissProt: P28329 ## Belongs to the carnitine/choline acetyltransferase family. View All» Brand Family: Chemicon View All» Product Name: Anti-Choline Acetyltransferase (ChAT) Antibody | AB143 View All» Concentration: Variable View All» Antibody Type: Polyclonal Antibody View All» Qty/Pk: 50 µL View All» Target / Localization: Cytoplasmic View All» Format: Serum View All» Host: Rabbit View All»
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