货号：AB1599

品牌：Merck Millipore

规格：

目录价：￥4212.00

市场价格：￥3580.20

会员价格：￥3369.60

金山科研平台，产品价格货期咨询微信：jinshanbio Description: Anti-Phosphotyrosine Antibody | AB1599 View All» Promotional Text: Special Shipping Offer on Antibodies100% Performance Guaranteed View All» Trade Name: Chemicon (Millipore) View All» Specificity: Specific for phosphotyrosine. No reaction with phosphoserine, phosphothreonine, serine, threonine, or tyrosine as confirmed by ELISA and Western blot using phosphorylated and unphosphosphorylated proteins. View All» Immunogen: A random amino acid polymer containing phosphotyrosine which was conjugated to Keyhole Limpet Hemocyanin. View All» Modifications: Phosphorylation View All» Species Reactivity: All View All» Application Notes: Western blot: 1:5,000 - 1:30,000 using colorimetric detection. Detection with more sensitive systems, such as chemiluminescence, may require higher antibody dilution. At a dilution of 1:1,000 the antibody was easily able to detect 10 ng (0.2 pmol) of a phosphorylated peptide using chromogenic (BCIP/NBT) development. Slot blot: 1:10,000. ELISA: 1:30,000 to 1;100,000 for detection of 1 μg/well of phosphotyrosine-containing substrate. Optimal working dilutions must be determined by end user. View All» Control: POSITIVE: EGF-stimulated A431 cells. View All» Presentation: Affinity purified immunoglobulin in 0.05m Tris with 0.15M NaCl, pH=7.2, and 0.02% sodium azide. View All» Storage Conditions: Maintain frozen at -20°C in undiluted aliquots. For working stocks, it is suggested to dilute the stock 20μL with a solution of 1% BSA in TBS (pH 7.4, that has been filtered through a 0.45μm membrane filter) to enhance the full recovery of the antibody from the supplied tube. Avoid repeated freeze/thaw. View All» Usage Statement: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. View All» Key Applications:

• ELISA

• Western Blotting
  View All» Brand Family: Chemicon View All» Product Name: Anti-Phosphotyrosine Antibody | AB1599 View All» Concentration: 5.0 mg/mL View All» Antibody Type: Polyclonal Antibody View All» Qty/Pk: 100 µg View All» Format: Affinity Purified View All» Host: Rabbit View All»
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