货号：AB1632

品牌：Merck Millipore

规格：100Ug

目录价：￥6588.00

市场价格：￥5599.80

会员价格：￥5270.40

金山科研平台，产品价格货期咨询微信：jinshanbio Description: Anti-Nitric Oxide Synthase I Antibody | AB1632 View All» Promotional Text: Special Shipping Offer on Antibodies100% Performance Guaranteed View All» Trade Name: Chemicon (Millipore) View All» Specificity: Reactive with human brain NOS-I (nNOS). Antibody binding is neutralized in Western blot by pre-adsorption with the immunogen peptide. This sequence is highly conserved in human, rat and mouse. View All» Immunogen: Synthetic peptide corresponding to amino acids 724-739 of human brain NOS, coupled to KLH. View All» Species Reactivity:

• Bovine

• Mouse

• Rat
  View All» Application Notes: Immunohistochemistry: Use at 5 - 10 μg/mL on rat neuronal sections fixed with 3.5% paraformaldehyde. Incubation overnight at 4°C with a 1:100 - 1:500 dilution. ABC (peroxidase) detection. Positive staining of Purkinje cells and Layer 5 primary neurons. Western blot: 1 - 10 μg/mL with chemiluminescent detection. Higher concentrations of the antibody may be necessary if colorimetric detection is used. Antibody dilution will also vary with the concentration of antigen. Then antibody should recognize a 155 kDa protein. ELISA: Use at 1.0 μg/mL or lower on plates coated with 50 - 100 ng of peptide antigen. Optimal working dilutions must be determined by the end user. View All» Presentation: Peptide affinity-purified immunoglobulin. Supplied at a 1mg/mL solution in PBS, pH 7.4, containing 0.1% BSA. View All» Storage Conditions: Maintain at -20°C in undiluted aliquots for up to 6 months. Avoid repeated thawing and refreezing. View All» UniProt Number: P29475 View All» Entrez Gene Number: NM_000620.1 View All» Gene Symbol:
  • NOS1

  • nNOS

  • NC-NOS

  • N-NOS

  • IHPS1

  • bNOS

  • NOS

  • EC 1.14.13.39
    View All» Alternate Names: NOS I; bNOS; nNOS View All» Usage Statement: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. View All» Key Applications:
    • ELISA

    • Western Blotting

    • Immunohistochemistry
      View All» UniProt Summary: FUNCTION: SwissProt: P29475 # Produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body. In the brain and peripheral nervous system, NO displays many properties of a neurotransmitter.COFACTOR: Heme group. & Binds 1 FAD. & Binds 1 FMN. & Metrahydrobiopterin (BH4). May stabilize the dimeric form of the enzyme.SIZE: 1434 amino acids; 160970 Da SUBUNIT: Homodimer. Interacts with DLG4; the interaction possibly being prevented by the association between NOS1 and CAPON. Forms a ternary complex with CAPON and RASD1. Forms a ternary complex with CAPON and SYN1. Interacts with ZDHHC23. Interacts with NOSIP; which may impair its synaptic location (By similarity).SUBCELLULAR LOCATION: Cell membrane, sarcolemma; Peripheral membrane protein. Cell projection, dendritic spine (By similarity). Note=In skeletal muscle, it is localized beneath the sarcolemma of fast-twitch muscle fiber by associating with the dystrophin glycoprotein complex. In neurons, enriched in dendritic spines (By similarity).TISSUE SPECIFICITY: Isoform 1 is ubiquitously expressed: detected in skeletal muscle and brain, also in testis, lung and kidney, and at low levels in heart, adrenal gland and retina. Not detected in the platelets. Isoform 3 is expressed only in testis. Isoform 4 is detected in testis, skeletal muscle, lung, and kidney, at low levels in the brain, but not in the heart and adrenal gland.DOMAIN: SwissProt: P29475 The PDZ domain in the N-terminal part of the neuronal isoform participates in protein-protein interaction, and is responsible for targeting nNos to synaptic membranes in muscles (By similarity).DISEASE: SwissProt: P29475 # Genetic variations in NOS1 gene are associated with susceptibility to infantile hypertrophic pyloric stenosis type 1 (IHPS1) [MIM:179010]. IHPS has an incidence of 1-5 per 1'000 live births in whites and a marked preponderance of males to females (4:1). IHPS is the most frequent disorder requiring surgery in the first year of life. The disorder is characterized by hypertrophy and hyperplasia of the circular muscle layer of the pylorus, leading to persistent vomiting 2-12 weeks after birth. Defective pyloric relaxation and increased pyloric smooth muscle mass have been suggested to be responsible for gastric-outlet obstruction.SIMILARITY: Belongs to the NOS family. & Contains 1 flavodoxin-like domain. & Contains 1 PDZ (DHR) domain. View All» Brand Family: Chemicon View All» Product Name: Anti-Nitric Oxide Synthase I Antibody | AB1632 View All» Antibody Type: Polyclonal Antibody View All» Qty/Pk: 100 µg View All» Format: Affinity Purified View All» Host: Rabbit View All»
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