货号：AB16501

品牌：Merck Millipore

规格：100Ug

目录价：￥5359.00

市场价格：￥4555.15

会员价格：￥4287.20

金山科研平台，产品价格货期咨询微信：jinshanbio Description: Anti-AIF Antibody, internal domain | AB16501 View All» Promotional Text: Special Shipping Offer on Antibodies100% Performance Guaranteed View All» Trade Name: Chemicon (Millipore) View All» Specificity: Apoptosis is characterized by several morphological nuclear changes including chromatin condensation and nuclear fragmentation. These changes are triggered by the activation of members of the caspase family, caspase activated DNase, and several novel proteins (Zamzami & Kroemer 1999). A novel gene, the product of which causes chromatin condensation and DNA fragmentation, was recently identified, cloned, and designated apoptosis inducing factor (AIF) (Susin et al. 1999). Like the critical molecules, cytochrome c and caspase 9, in apoptosis, AIF localizes in mitochondria. AIF translocates to the nucleus when apoptosis is induced and induces mitochondria to release the apoptogenic proteins, cytochrome c and caspase-9. AIF induces chromatin condensation and DNA fragmentation, which are the hallmarks of apoptosis, of the isolated nucleus and the nucleus in live cells by microinjection. AIF is highly conserved between human and mouse and widely expressed (Susin et al. 1999). View All» Molecular Weight: 67 kDa View All» Epitope: internal domain View All» Immunogen: Rabbit anti-AIF (Internal region) polyclonal antibody was raised against a peptide corresponding to amino acids 517 to 531 of human AIF (Susin et al. 1999). This sequence is identical to those of mouse and rat AIF (Susin et al. 1999). View All» Background Information: Apoptosis is characterized by several morphological nuclear changes including chromatin condensation and nuclear fragmentation. These changes are triggered by the activation of members of the caspase family, caspase activated DNase, and several novel proteins (Zamzami et al., 1999). A novel gene, the product of which causes chromatin condensation and DNA fragmentation, was recently identified, cloned, and designated apoptosis inducing factor (AIF) (Susin et al., 1999). Like the critical molecules, cytochrome c and caspase 9, in apoptosis, AIF localizes in mitochondria. AIF translocates to the nucleus when apoptosis is induced and induces mitochondria to release the apoptogenic proteins, cytochrome c and caspase-9. AIF induces chromatin condensation and DNA fragmentation, which are the hallmarks of apoptosis, of the isolated nucleus and the nucleus in live cells by microinjection. AIF is highly conserved between human and mouse and widely expressed (Susin et al., 1999). View All» Species Reactivity:

• Human

• Mouse

• Rat
  View All» Application Notes: Western blot: 0.25 to 1 μg/mL. K562 cell lysate can be used as a positive control and a 67 kDa band should be detected. Immunohistochemistry on formalin fixed, paraffin sections: 5-20 μg/ml, with antigen retrieval; see website protocol for details and photographs. Optimal working dilutions must be determined by end user. View All» Control: Widely expressed View All» Purification Method: Ammonium sulfate precipitation and DEAE-cellulose chromatography View All» Presentation: Purified IgG in PBS containing 0.02% sodium azide. View All» Storage Conditions: Maintain for 1 year at 2–8°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. View All» UniProt Number: O95831 View All» Entrez Gene Number:
  • NM_004208.2

  • NM_145812.1

  • NM_145813.1
    View All» Gene Symbol:
    • AIFM1

    • MGC111425

    • PDCD8

    • AIF

    • EC 1.-.-.-
      View All» Alternate Names: Apoptosis Inducing Factor View All» Usage Statement: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. View All» Key Applications:
      • Immunohistochemistry (Paraffin)

      • Western Blotting
        View All» Entrez Gene Summary: This gene encodes a flavoprotein essential for nuclear disassembly in apoptotic cells that is found in the mitochondrial intermembrane space in healthy cells. Induction of apoptosis results in the translocation of this protein to the nucleus where it effects chromosome condensation and fragmentation. In addition, this gene product induces mitochondria to release the apoptogenic proteins cytochrome c and caspase-9. Three alternative transcripts encoding different isoforms have been identified for this gene. View All» UniProt Summary: FUNCTION: SwissProt: O95831 # Probable oxidoreductase that acts as a caspase- independent mitochondrial effector of apoptotic cell death. Extramitochondrial AIF induces nuclear chromatin condensation and large scale DNA fragmentation (in vitro).COFACTOR: FAD.SIZE: 613 amino acids; 66901 Da SUBUNIT: Binds to DNA in a sequence-independent manner.SUBCELLULAR LOCATION: Mitochondrion intermembrane space. Nucleus. Note=Translocated to the nucleus upon induction of apoptosis.SIMILARITY: SwissProt: O95831 ## Belongs to the FAD-dependent oxidoreductase family. View All» Brand Family: Chemicon View All» Product Name: Anti-AIF Antibody, internal domain | AB16501 View All» Concentration: 1 mg/mL View All» Antibody Type: Polyclonal Antibody View All» Qty/Pk: 100 μg View All» Format: Purified View All» Host: Rabbit View All»
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