货号：AB16901

品牌：Merck Millipore

规格：250Ug

目录价：￥5053.00

市场价格：￥4295.05

会员价格：￥4042.40

金山科研平台，产品价格货期咨询微信：jinshanbio Description: Anti-Green Fluorescent Protein Antibody | AB16901 View All» Promotional Text: Special Shipping Offer on Antibodies100% Performance Guaranteed View All» Trade Name: Chemicon (Millipore) View All» Specificity: AB16901 is made against highly-purified native GFP from Aequorea victoria. It is reactive with GFP from both native and recombinant sources. View All» Molecular Weight: MW for GFP is 27 kDa. Dependent upon the molecular weight of the fusion protein being detected. View All» Immunogen: A recombinant green fluorescent protein (GFP) containing a 6-his tag, was highly purified by affinity chromatography on Nickel column followed by chromatography on a P-100 gel filtration column. The resulting product was present as only a singl View All» Background Information: The Green Fluorescent Protein (GFP) from the jellyfish Aequorea victoria is used as a fluorescent indicator for monitoring gene expression in a variety of cellular systems, including living organisms and fixed tissues. Unlike other bioluminescent reporters, GFP fluoresces in the absence of substrates, cofactors, or other intrinsic or extrinsic proteins. Purified GFP is a 27 kDa monomer consisting of 238 amino acids and emits green light (emission maximum at 509 nm) when excited with blue or UV light. View All» Species Reactivity: Vertebrates View All» Application Notes: Immunoblotting: AB16901 detects a band of the proper molecular weight (30kDa) in lysates from E.coli expressing GFP but not in E.coli that do not express or in lysates prepared from E.coli containing the GFP-expressing plasmid but not induced, detect GFP in lysates from COS or primary neurons or fibroblast cells transfected with plasmids directing expression GFP or a tau-GFP fusion protein. Did not detect any signal when 50μg of protein from a rat brain lysate (negative control) was fractionated on the SDS-PAGE. Titers were 10-20,000 when detected with an anti-chicken horseradish peroxidase-conjugated secondary by ECL or by a colorimetric assay using DAB. Immunocytochemistry: GFP can be detected in cells that are transfected with a plasmid directing the expression of either GFP or a GFP-Fusion protein. Cells that do not express GFP exhibit no detectable staining. AB binding was determined using HRP conjugated-anti chicken secondary Abs and visualized using DAB. The anti-GFP Abs were used at 1-2,500-5,000 fold dilution. Cells were fixed using 4% formaldehyde in PBS (pH7.4). For immunocytochemistry, the cultures were fixed in 4% formaldehyde in PBS pH7.4, for 20 minutes at room temperature. The cells are permeabilized with 0.2% Triton X-100 and 2% serum (corresponding to the aminal in which the secondary antibody was raised) in PBS for 20 minutes at room temperature. Cell cultures are then incubated with primary antisera in PBS containing 2% serum (as above), at 4°C, overnight. Antibody binding is detected using an avidin/biotin/peroxidase detection system. Cultures were washed four times (10 minutes each) between steps, and reaction product is developed with a 0.05% diaminobenzidine / 0.02% hydrogen peroxide solution on ice for 8-10 minutes. AB16901 has been used to detect GFP in primary neurons and zebrafish fibroblasts. Immunoprecipitation: 5μg antibody per no more than 500μl of cell lysate. It is important to use a rabbit anti-chicken secondary for capture or anti-chicken beads as Chicken IgG will not react with protein A or protein G. Optimal working dilutions must be determined by the end user. View All» Control: GFP fusion protein expressed in cells View All» Purification Method: Ammonium sulfate precipitation View All» Presentation: Purified chicken immunoglobulin in TBS, pH 7.5 with 0.02% sodium azide. View All» Storage Conditions: The undiluted antibody solution is stable for approximately 6 months stored between 2 and 8°C. During shipment, small volumes of product will occasionally become entrapped in the seal of the product vial. For products with volumes of 200μL or less, we recommend gently tapping the vial on a hard surface or briefly centrifuging the vial in a tabletop centrifuge to dislodge any liquid in the container's cap. View All» UniProt Number: P42212 View All» Alternate Names: GFP; eGFP View All» Usage Statement: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. View All» Key Applications:

• Immunocytochemistry

• Immunoprecipitation

• Western Blotting
  View All» Brand Family: Chemicon View All» Product Name: Anti-Green Fluorescent Protein Antibody | AB16901 View All» Concentration: 1 mg/mL View All» Antibody Type: Polyclonal Antibody View All» Qty/Pk: 250 μg View All» Format: Purified View All» Host: Chicken View All»
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