货号：AB16951

品牌：Merck Millipore

规格：100Ug

目录价：￥5053.00

市场价格：￥4295.05

会员价格：￥4042.40

金山科研平台，产品价格货期咨询微信：jinshanbio Description: Anti-STAT1-α Antibody | AB16951 View All» Promotional Text: Special Shipping Offer on Antibodies100% Performance Guaranteed View All» Trade Name: Chemicon (Millipore) View All» Specificity: STAT's (signal transducers and activators of transcripition) are a family of cytoplasmic latent transription factors that are activated to regulate gene expression in response to a large number of extracellular signaling polypeptides including cytokines, interferons, and growth factors. After phosphorylation by JAK tyrosine kinases, STATs enter the nucleus to regulate transcription of many different genes. Among the seven STATs (Stat1, Stat2, Stat3, Stat4, Stat5, Stat5beta, and Stat6), Stat1, Stat3, Stat5alpha, and Stat5beta have a wide activation profile (Leonard et al. 1998; Schindler et al. 1995). STAT1 is activated by many different ligands including IFN family (IFN-alpha, IFN-beta, IFN-gamma, and IL-10), gp130 family (IL-6, IL-11, LIF, CNTF and G-CSF), and receptor tyrosine kinases (EGF, PDGF, CSF-1) (Darnell 1997). STAT1 has two forms, the 91 kDa STAT1alpha and the 84 kDa STAT1beta, which are encoded by the same gene with splicing variant (Schindler et al. 1992). View All» Immunogen: Polyclonal antibody raised against a peptide corresponding to amino acids 712-750 of human STAT1alpha (Schindler et al. 1992). The corresponding sequences of mouse differ from those of the immunogenic peptide by four amino acids. View All» Species Reactivity:

• Human

• Mouse
  View All» Application Notes: Western blot: 1:1000-1:2000 Immunoprecipitation: 2 to 4 μg Whole cell lysate from HeLa , Jurkat, or A431 cells can be used as a positive control for Western Blot and a 91 kDa band can be detected. No reaction to the 84 kDa STAT1beta. Optimal working dilutions must be determined by end user. View All» Presentation: Affinity purified immunoglobulin. Liquid in PBS containing 0.02% sodium azide. View All» Storage Conditions: Maintain refrigerated at 2-8°C in undiluted aliquots for up to 12 months. View All» UniProt Number: P42224 View All» Entrez Gene Number:
  • NM_007315.2

  • NM_139266.1
    View All» Gene Symbol:
    • STAT1

    • ISGF-3

    • STAT91

    • DKFZp686B04100
      View All» Usage Statement: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. View All» Key Applications:
      • Immunoprecipitation

      • Western Blotting
        View All» Entrez Gene Summary: The protein encoded by this gene is a member of the STAT protein family. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein can be activated by various ligands including interferon-alpha, interferon-gamma, EGF, PDGF and IL6. This protein mediates the expression of a variety of genes, which is thought to be important for cell viability in response to different cell stimuli and pathogens. Two alternatively spliced transcript variants encoding distinct isoforms have been described. View All» UniProt Summary: FUNCTION: SwissProt: P42224 # Signal transducer and activator of transcription that mediates signaling by interferons (IFNs). Following type I IFN (IFN-alpha and IFN-beta) binding to cell surface receptors, Jak kinases (TYK2 and JAK1) are activated, leading to tyrosine phosphorylation of STAT1 and STAT2. The phosphorylated STATs dimerize, associate with ISGF3G/IRF-9 to form a complex termed ISGF3 transcription factor, that enters the nucleus. ISGF3 binds to the IFN stimulated response element (ISRE) to activate the transcription of interferon stimulated genes, which drive the cell in an antiviral state. In response to type II IFN (IFN-gamma), STAT1 is tyrosine- and serine-phosphorylated. It then forms a homodimer termed IFN-gamma-activated factor (GAF), migrates into the nucleus and binds to the IFN gamma activated sequence (GAS) to drive the expression of the target genes, inducing a cellular antiviral state.SIZE: 750 amino acids; 87335 Da SUBUNIT: Isoform alpha homodimerizes upon IFN-gamma induced phosphorylation. Heterodimer with STAT2 upon IFN-alpha/beta induced phosphorylation. Interacts with NMI. Interacts with Sendai virus C', C, Y1 and Y2 proteins, Nipah virus P, V and W proteins, and rabies virus phosphoprotein preventing activation of ISRE and GAS promoter (By similarity). Interaction with HCV core protein results in STAT1 degradation.SUBCELLULAR LOCATION: Cytoplasm. Nucleus. Note=Translocated into the nucleus upon activation by IFN-alpha/beta.PTM: Phosphorylated on tyrosine residues in response to IFN-alpha, IFN-gamma, PDGF and EGF. Serine phosphorylation is also required for maximal transcriptional activity in IFN-gamma transduction (lacking in beta form).DISEASE: SwissProt: P42224 # Defects in STAT1 are the cause of STAT1 deficiency [MIM:600555]. Patients generally suffer from mycobacterial or viral diseases. In the case of complete deficiency, patients can die of viral disease. & Defects in STAT1 are a cause of mendelian susceptibility to mycobacterial disease (MSMD) [MIM:209950]; also known as familial disseminated atypical mycobacterial infection. This rare condition confers predisposition to illness caused by moderately virulent mycobacterial species, such as Bacillus Calmette-Guerin (BCG) vaccine and environmental non-tuberculous mycobacteria, and by the more virulent Mycobacterium tuberculosis. Other microorganisms rarely cause severe clinical disease in individuals with susceptibility to mycobacterial infections, with the exception of Salmonella which infects less than 50% of these individuals. The pathogenic mechanism underlying MSMD is the impairment of interferon-gamma mediated immunity whose severity determines the clinical outcome. Some patients die of overwhelming mycobacterial disease with lepromatous-like lesions in early childhood, whereas others develop, later in life, disseminated but curable infections with tuberculoid granulomas. MSMD is a genetically heterogeneous disease with autosomal recessive, autosomal dominant or X-linked inheritance.SIMILARITY: SwissProt: P42224 ## Belongs to the transcription factor STAT family. & Contains 1 SH2 domain. View All» Brand Family: Chemicon View All» Product Name: Anti-STAT1-α Antibody | AB16951 View All» Concentration: 1mg/mL View All» Antibody Type: Polyclonal Antibody View All» Qty/Pk: 100 µg View All» Format: Purified View All» Host: Rabbit View All»
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