货号：AB1722

品牌：Merck Millipore

规格：100UL

目录价：￥5666.00

市场价格：￥4816.10

会员价格：￥4532.80

金山科研平台，产品价格货期咨询微信：jinshanbio Description: Anti-Bcl-2 Antibody, a.a. 68-86 of mBCL2 | AB1722 View All» Promotional Text: Special Shipping Offer on Antibodies100% Performance Guaranteed View All» Trade Name: Chemicon (Millipore) View All» Specificity: AB1722 recognizes Bcl-2 alpha and beta isoforms in mouse and rat. Cross reactivity with other species is unknown View All» Epitope: a.a. 68-86 of mBCL2 View All» Immunogen: Peptide against mouse Bcl-2 a.a. 68-86 RTSPLRPLVATAGPALSPV View All» Background Information: In C. elegans, ced-3 and ced-4 were identified as genes essential for programmed cell death, while ced-9 was found to be a regulator of cell death by preventing apoptosis. The first mammalian homolog for ced-3 was described in 1988 as Bcl-2 which is involved in B-cell lymphomas (Vaux and Adams, 1988, Nature 335: 440). Bcl-2 transfected B cells were shown to be resistant towards apoptosis normally induced in B cells by IL-3 withdrawal: for the first time it was shown that the pathway toward tumorigenesis depends not only on the ability to escape growth control but also depends on the ability to prevent apoptosis. The bcl-2 gene codes for a 25 kDa protein. The C terminal 21 amino acids encode a stretch of hydrophobic amino acids that are important in membrane docking: Bcl-2 resides on the cytoplasmic face of the mitochondrial outer membrane, the nuclear envelop, and the endoplasmic reticulum. Deletion of the C terminus does not abrogate Bcl-2 survival function! Most Bcl-2 homologs have this hydrophobic C terminal domain, though they not necessarily are located on membranes but are cytosolic (e.g. Bax). {http://www.celldeath.de/encyclo/} View All» Species Reactivity:

• Mouse

• Rat
  View All» Application Notes: Immunoblotting: Recommended antibody dilution 1:1500-3000 Immunoprecipitation: Recommended antibody dilution 1:50 Immunohistochemistry: Recommended antibody dilution 1:600-1500 Optimal antibody dilutions must be determined by end-user. View All» Presentation: Whole serum without preservatives View All» Storage Conditions: Store at -20°C for up to 12 months from date of receipt. Avoid repeated freeze thaws. View All» UniProt Number: P10415 View All» Entrez Gene Number:
  • NM_000633.2

  • NM_000657.2
    View All» Gene Symbol:
    • BCL2

    • Bcl-2
      View All» Usage Statement: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. View All» Key Applications:
      • Western Blotting

      • Immunoprecipitation

      • Immunohistochemistry
        View All» Entrez Gene Summary: This gene encodes an integral outer mitochondrial membrane protein that blocks the apoptotic death of some cells such as lymphocytes. Constitutive expression of BCL2, such as in the case of translocation of BCL2 to Ig heavy chain locus, is thought to be the cause of follicular lymphoma. Two transcript variants, produced by alternate splicing, differ in their C-terminal ends. View All» UniProt Summary: FUNCTION: SwissProt: P10415 # Suppresses apoptosis in a variety of cell systems including factor-dependent lymphohematopoietic and neural cells. Regulates cell death by controlling the mitochondrial membrane permeability. Appears to function in a feedback loop system with caspases. Inhibits caspase activity either by preventing the release of cytochrome c from the mitochondria and/or by binding to the apoptosis-activating factor (APAF-1).SIZE: 239 amino acids; 26266 Da SUBUNIT: Forms homodimers, and heterodimers with BAX, BAD, BAK and Bcl-X(L). Heterodimerization with BAX requires intact BH1 and BH2 motifs, and is necessary for anti-apoptotic activity (By similarity). Also interacts with APAF1, RAF-1, TP53BP2, BBC3, BCL2L1, MRPL41 and BNIPL. Binding to FKBP8 seems to target BCL2 to the mitochondria and probably interferes with the binding of BCL2 to its targets.SUBCELLULAR LOCATION: Mitochondrion outer membrane; Single-pass membrane protein. Nucleus membrane; Single-pass membrane protein. Endoplasmic reticulum membrane; Single-pass membrane protein.TISSUE SPECIFICITY: Expressed in a variety of tissues.DOMAIN: SwissProt: P10415 The BH4 motif is required for anti-apoptotic activity and for interaction with RAF-1.PTM: Phosphorylation/dephosphorylation on Ser-70 regulates anti- apoptotic activity. Growth factor-stimulated phosphorylation on Ser-70 by PKC is required for the anti-apoptosis activity and occurs during the G2/M phase of the cell cycle. In the absence of growth factors, BCL2 appears to be phosphorylated by other protein kinases such as ERKs and stress-activated kinases. Dephosphorylated by protein phosphatase 2A (PP2A) (By similarity). & Proteolytically cleaved by caspases during apoptosis. The cleaved protein, lacking the BH4 motif, has pro-apoptotic activity, causes the release of cytochrome c into the cytosol promoting further caspase activity.DISEASE: SwissProt: P10415 # A chromosomal aberration involving BCL2 may be a cause of follicular lymphoma (FL) [MIM:151430]; also known as type II chronic lymphatic leukemia. Translocation t(14;18)(q32;q21) with immunoglobulin gene regions. BCL2 mutations found in non-Hodgkin lymphomas carrying the chromosomal translocation could be attributed to the Ig somatic hypermutation mechanism resulting in nucleotide transitions.SIMILARITY: Belongs to the Bcl-2 family. View All» Brand Family: Chemicon View All» Product Name: Anti-Bcl-2 Antibody, a.a. 68-86 of mBCL2 | AB1722 View All» Antibody Type: Polyclonal Antibody View All» Qty/Pk: 100 µL View All» Format: Serum View All» Host: Rabbit View All»
        © 金山科研平台是专业的授权总代理区域代理经销平台。
        © 如需询价，请加客服QQ：1749072012 、客服微信：jinshanbio，或发送邮件到1749072012@qq.com
        © 平台为生命科学研究相关领域提供一站式耗材试剂仪器解决方案和采购服务，数据资源基于CC协议。
        © 本文地址：https://www.16ao.com/thread-152614.htm
        © Merck Millipore代理AB1722 Anti-Bcl-2 Antibody, a.a. 68-86 of mBCL2;store at -20℃，产品报价联系微信jinshanbio