货号：AB1761

品牌：Merck Millipore

规格：

目录价：￥5468.00

市场价格：￥4647.80

会员价格：￥4374.40

金山科研平台，产品价格货期咨询微信：jinshanbio Description: Anti-Protein Gene Product 9.5 Antibody | AB1761 View All» Promotional Text: Special Shipping Offer on Antibodies100% Performance Guaranteed View All» Trade Name: Chemicon (Millipore) View All» Specificity: Protein Gene Product 9.5 (PGP9.5, Ubiquitin C-terminal Hydrolase, UCH-L1) is an abundant protein in many tissues, but especially so in neurons where it has been effectively used as a phenotypic marker. PGP9.5 is known to be a member of the ubiquitin C-terminal hydrolase family and immunohistochemical studies have shown that the protein is enriched in several ubiquinated inclusion bodies, suggesting that such structures may be metabolically dynamic regions of the cell. View All» Molecular Weight: 24-27 kDa View All» Immunogen: Recombinant human UCH-L1. View All» Background Information: Protein Gene Product 9.5 (PGP9.5) is an abundant protein in many tissues, but especially so in neurons where it has been effectively used as a phenotypic marker and is immunologically distinct from neuron specific enolase. The 27 kDa protein was first identified by high resolution two dimensional PAGE. Standard immunohistochemical techniques have demonstrated the presence of PGP9.5 in neurons and nerve fibers at all levels of the central and peripheral nervous system, in many neuroendocrine cells, in segments of the renal tubules, in spermatogonia and Leydig cells of the testis, in ova and in some cells of both the pregnant and non-pregnant corpus luteum. PGP9.5 is known to be a member of the ubiquitin C-terminal hydrolase family and immunohistochemical studies have shown that the protein is enriched in several ubiquinated inclusion bodies, suggesting that such structures may be metabolically dynamic regions of the cell. View All» Species Reactivity:

• Human

• Mouse

• Rat
  View All» Species Reactivity Note: Human, rat and mouse. Other species have not been tested. View All» Application Notes: Western Blot: 1:1,000. Recognizes a 24-27 kDa protein. Immunohistochemistry (paraffin): Citrate pH 6.0 antigen retrieval followed by incubation with the antibody at a dilution of 1:50 - 1:500.Optimal working dilutions must be determined by the end user. View All» Control: Brain tissue. View All» Quality Assurance: Routinely evaluated by Western Blot on Mouse Brain lysates.Western Blot Analysis: 1:1000 dilution of this lot detected PROTEIN GENE PRODUCT 9.5 on 10 μg of Mouse Brain lysates. View All» Purification Method: Unpurified View All» Presentation: Rabbit polyclonal serum in buffer containing 0.01 M sodium azide. View All» Storage Conditions: Stable for 1 year at -20°C in undiluted aliquots (not less than 10 μL) from date of receipt. The use of high quality “antiserum-grade” plastic or glass vials is recommended.Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. View All» UniProt Number: P09936 View All» Entrez Gene Number: NP_004172 View All» Gene Symbol:
  • UCHL1

  • PARK5

  • UCH-L1

  • Uch-L1

  • PGP9.5

  • EC 3.4.19.12

  • EC 6.-.-.-
    View All» Alternate Names:
    • Ubiquitin C-terminal Hydrolase

    • PGP 9.5

    • UCH-L1
      View All» Usage Statement: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. View All» Key Applications:
      • Immunohistochemistry

      • Immunohistochemistry (Paraffin)

      • Western Blotting
        View All» Entrez Gene Summary: UCHL1 is a member of a gene family whose products hydrolyze small C-terminal adducts of ubiquitin to generate the ubiquitin monomer. Expression of UCHL1 is highly specific to neurons and to cells of the diffuse neuroendocrine system and their tumors. It is present in all neurons (Doran et al., 1983 [PubMed 6343558]).[supplied by OMIM] View All» UniProt Summary: FUNCTION: SwissProt: P09936 # Ubiquitin-protein hydrolase involved both in the processing of ubiquitin precursors and of ubiquitinated proteins. This enzyme is a thiol protease that recognizes and hydrolyzes a peptide bond at the C-terminal glycine of ubiquitin. Also binds to free monoubiquitin and may prevent its degradation in lysosomes. The homodimer may have ATP-independent ubiquitin ligase activity.SIZE: 223 amino acids; 24824 Da SUBUNIT: Homodimer. Interacts with SNCA (By similarity). Interacts with COPS5.SUBCELLULAR LOCATION: Cytoplasm.TISSUE SPECIFICITY: Found in neuronal cell bodies and processes throughout the neocortex (at protein level). Expressed in neurons and cells of the diffuse neuroendocrine system and their tumors. Weakly expressed in ovary.PTM: O-glycosylated (By similarity).DISEASE: SwissProt: P09936 # Oxidation of Met-1, Met-6, Met-12, Met-124 and Met-179 to methionine sulfoxide, and oxidation of Cys-220 to cysteine sulfonic acid have been observed in brains from Alzheimer disease (AD) and Parkinson disease (PD) patients. In AD, UCHL1 was found to be associated with neurofibrillary tangles.SIMILARITY: SwissProt: P09936 ## Belongs to the peptidase C12 family.MISCELLANEOUS: In contrast to UCHL3, does not hydrolyze a peptide bond at the C-terminal glycine of NEDD8. View All» Brand Family: Chemicon View All» Product Name: Anti-Protein Gene Product 9.5 Antibody | AB1761 View All» Concentration: Variable View All» Antibody Type: Polyclonal Antibody View All» Qty/Pk: 50 µL View All» Format: Serum View All» Host: Rabbit View All»
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