金山科研平台，产品价格货期咨询微信：jinshanbio Assay Type:ELISA Development Kit for phosphorylated intracellular proteins Assay Length:4 hours 20 minutes (after plate preparation) Sample Type & Volume Required:Cell lysates (100 µL) Sufficient Materials:Kits available for two, five, or fifteen 96-well plates* Specificity:Please see the product datasheet Molecule Information: DDR1 Long Name: Discoidin Domain Receptor 1 Aliases: CAK; CD167a; RTK6; TrkE Entrez Gene IDs: 780 (Human) Background: Discoidin Domain Receptor 1 DDR1, also known as CAK, CD167a, RTK6, and TrkE, is a 120-140 kDa type I transmembrane glycoprotein that belongs to the discoidin-like domain containing subfamily of receptor tyrosine kinases and serve as receptors for collagen. DDR1 is expressed on epithelial tissues, activated monocytes and neutrophils, and in several cancers. Compared to isoform DDR1b, DDR1a lacks 37 aa’s that include a Shc-interacting NPxY motif in the cytoplasmic juxtamembrane region. Two additional kinase deficient splice forms are expressed in colon cancer. The discoidin-like domain mediates binding to collagens I-V. DDR1 selectively recognizes the triple helical structure of collagen. It is expressed on the cell surface as a dimer which can include different isoforms. DDR1 oligomerization enhances collagen binding and also modulates collagen fibrillogenesis. The transmembrane segment contains a leucine zipper and GxxxG motif, but neither is exclusively required for dimerization. Collagen binding induces prolonged autophosphorylation, including the NPxY motif. Collagen binding also results in the proteolytic cleavage of a tyrosine phosphorylated 60 kDa C-terminal fragment (CTF), and a 60 kDa ECD fragment. TIMP-3 and TAPI-1 inhibit shedding of the ECD fragment but not the CTF. Overexpression of DDR1a promotes MMP-2 activation and results in an increased invasiveness of a glioblastoma cell line; DDR1b does not.