货号：AB19166

品牌：Merck Millipore

规格：

目录价：￥3978.00

市场价格：￥3381.30

会员价格：￥3182.40

金山科研平台，产品价格货期咨询微信：jinshanbio Description: Anti-ADAMTS-4 Antibody, C-terminus | AB19166 View All» Promotional Text: Special Shipping Offer on Antibodies100% Performance Guaranteed View All» Trade Name: Chemicon (Millipore) View All» Specificity: In lysates of primary human chondrocytes differentiated in alginate beads in the presence of TGF-beta, the Rabbit Anti-ADAMTS-4 Polyclonal Antibody recognizes a band of 110 kD, consistent with full-length ADAMTS-4. A minor band at 40 kD is also recognized. View All» Epitope: C-terminus View All» Immunogen: A synthetic peptide derived from the C-terminal region of human ADAMTS-4 View All» Background Information: The ADAMTS (A Disintegrin and Metalloprotease with Thrombospondin motifs) family of secreted proteases comprises at least 20 members in mammals, and several members in invertebrates. ADAMTS members resemble the membrane-bound ADAM family of proteases in the presence of a prodomain, a catalytic domain and a disintegrin-like domain. However, ADAMTS members lack a transmembrane domain, and instead contain varying numbers of thrombospondin type I modules (Tang, 2001). ADAMTS-4 was first purified by its ability to cleave aggrecan (Tortorella et al., 1999), a major cartilage proteoglycan that is degraded in patients with osteoarthritis and rheumatoid arthritis. ADAMTS-4 cleaves aggrecan between Glu373 and Ala374, a physiological cleavage site in arthritic cartilage. In addition, expression of ADAMTS-4 is elevated in chondrocytes treated with inflammatory cytokines interleukin-1, tumor necrosis factor and oncostatin M (Koshy et al., 2002; Tortorella et al., 2001). Activation of the aggrecanase activity of ADAMTS-4 requires cleavage of the prodomain by a furin-like protease and the C-terminal domain by an as-yet-unidentified matrix metalloproteinase (Gao et al., 2002). Inclusion of protease inhibitors, including EDTA or other metalloprotease inhibitor, in the lysis buffer is recommended to inhibit proteolysis during lysate preparation. View All» Species Reactivity: Human View All» Application Notes: Western blot: 1:200-500 Optimal working dilutions must be determined by the end user. View All» Presentation: Affinity purified immunoglobulin. 1 mg/ml in PBS containing 50% glycerol and 0.05% sodium azide. View All» Storage Conditions: Maintain frozen at -20ºC for up to 12 months. During shipment, small volumes of product will occasionally become entrapped in the seal of the product vial. For products with volumes of 200 μL or less, we recommend gently tapping the vial on a hard surface or briefly centrifuging the vial in a tabletop centrifuge to dislodge any liquid in the container's cap. View All» UniProt Number: O75173 View All» Entrez Gene Number: NM_005099.4 View All» Gene Symbol:

• ADAMTS4

• KIAA0688

• ADAM-TS4

• Aggrecanase-1

• aggrecanase-1

• ADAMTS-2

• ADAMTS-4

• ADMP-1

• EC 3.4.24.82
  View All» Alternate Names: Aggrecanase 1 View All» Usage Statement: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. View All» Key Applications: Western Blotting View All» Entrez Gene Summary: This gene encodes a member of the ADAMTS (a disintegrin and metalloproteinase with thrombospondin motifs) protein family. Members of the family share several distinct protein modules, including a propeptide region, a metalloproteinase domain, a disintegrin-like domain, and a thrombospondin type 1 (TS) motif. Individual members of this family differ in the number of C-terminal TS motifs, and some have unique C-terminal domains. The enzyme encoded by this gene lacks a C-terminal TS motif. It is responsible for the degradation of aggrecan, a major proteoglycan of cartilage, and brevican, a brain-specific extracellular matrix protein. The cleavage of aggrecan and brevican suggests key roles of this enzyme in arthritic disease and in the central nervous system, potentially, in the progression of glioma. View All» UniProt Summary: FUNCTION: SwissProt: O75173 # Cleaves aggrecan, a cartilage proteoglycan, and may be involved in its turnover. May play an important role in the destruction of aggrecan in arthritic diseases. Could also be a critical factor in the exacerbation of neurodegeneration in Alzheimer disease. Cleaves aggrecan at the '392-Glu- -Ala-393' site.COFACTOR: Binds 1 zinc ion per subunit (By similarity).SIZE: 837 amino acids; 90225 Da SUBCELLULAR LOCATION: Secreted, extracellular space, extracellular matrix (By similarity).TISSUE SPECIFICITY: Expressed in brain, lung and heart. Expressed at very low level in placenta and skeletal muscles.DOMAIN: SwissProt: O75173 The spacer domain and the TSP type-1 domains are important for a tight interaction with the extracellular matrix. & The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.PTM: The precursor is cleaved by a furin endopeptidase.SIMILARITY: Contains 1 disintegrin domain. & Contains 1 peptidase M12B domain. & Contains 1 TSP type-1 domain. View All» Brand Family: Chemicon View All» Product Name: Anti-ADAMTS-4 Antibody, C-terminus | AB19166 View All» Concentration: 1 mg/mL View All» Antibody Type: Polyclonal Antibody View All» Qty/Pk: 100 µg View All» Format: Affinity Purified View All» Host: Rabbit View All»
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