金山科研平台，产品价格货期咨询微信：jinshanbio Assay Type:ELISA Development Kit for phosphorylated intracellular proteins Assay Length:4 hours 40 minutes (after plate preparation) Sample Type & Volume Required:Cell lysates (100 µL) Sufficient Materials:Kits available for two, five, or fifteen 96-well plates* Range:937.5 - 60,000 pg/mL Specificity:Please see the product datasheet Molecule Information: PRAS40 Long Name: 40 kDa Proline-rich Akt1 Substrate Aliases: AKT1S1; Lobe Entrez Gene IDs: 84335 (Human); 67605 (Mouse); 292887 (Rat) Background: PRAS40 PRAS40 (40 kDa proline-rich Akt1 substrate), also known as AKT1S1, is a widely expressed component of the cytoplasmic mTOR complex (mTORC1). In response to insulin stimulation, Akt1 phosphorylation of PRAS40 relieves the basal PRAS40-mediated inhibition of mTORC1. Activated mTORC1 is a key effector of the cell survival pathway. PRAS40 phosphorylation by Akt3 or Pim1 similarly exerts an anti-apoptotic effect during tumor progression and neuronal ischemia . Alternate splicing generates an isoform that lacks the N-terminal 130 amino acids.