金山科研平台，产品价格货期咨询微信：jinshanbio Source:E. coli-derived, Accession #:P62837 Purity:>95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain. Predicted Molecular Mass:17 kDa Activity:Recombinant Human UbcH5b/UBE2D2 is a member of the Ubiquitin-conjugating (E2) enzyme family that receives Ubiquitin from a Ubiquitin-activating (E1) enzyme and subsequently interacts with a Ubiquitin ligase (E3) to conjugate Ubiquitin to substrate proteins. Reaction conditions will need to be optimized for each specific application. We recommend an initial Recombinant Human UbcH5b/UBE2D2 concentration of 0.1-1 µM. Formulation:Supplied as a solution in HEPES, NaCl, Glycerol.See Certificate of Analysis for details. Molecule Information: UbcH5b/UBE2D2 Aliases: PUBC1; UBC5B; UBE2D2 Entrez Gene IDs: 7322 (Human); 56550 (Mouse); 641452 (Rat) Background: Ubch5b Ubiquitin-conjugating Enzyme H5b (UbcH5b), also known as Ubiquitin-conjugating Enzyme E2D 2 (UBE2D2), is a widely expressed member of the Ubiquitin-conjugating (E2) enzyme family. The protein has a predicted molecular weight of 16.5 kDa. UbcH5b/UBE2D2 localizes to both the nucleus and the cytoplasm. The human protein shares 100% and 92% amino acid sequence identity with the mouse and rat orthologs, respectively. This enzyme has an E2 catalytic core domain that contains an active site cysteine residue that is required for the formation of a thioester bond with Ubiquitin. UbcH5b/UBE2D2 is capable of mediating the formation of Ubiquitin chains linked through Lys11, Lys48, or Lys63. Working with the SCF(Fbxw2) and MDM2/HDM2 Ubiquitin ligases (E3s), UbcH5b/UBE2D2 mediates the ubiquitination and degradation of the transcription factors GCM1 and p53, respectively. Along with UBE2N/Ubc13, UbcH5b/UBE2D2 may have a role in the endocytosis and endolysosomal degradation of MHC class I molecules. Non-proteolytic ubiquitination of TRIM5-alpha by UbcH5b/UBE2D2 has been reported to block HIV reverse transcription. Pathologically, UBE2D family members may be critical targets of cadmium during cadmium-induced renal toxicity. Additionally, overexpression of UbcH5b/UBE2D2 has been linked to inflammatory bowel disease.