金山科研平台，产品价格货期咨询微信：jinshanbio Source:E. coli-derived, Accession #:P61077 Purity:>95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain. Predicted Molecular Mass:17 kDa Activity:Recombinant Human UbcH5c/UBE2D3 is a member of the Ubiquitin-conjugating (E2) enzyme family that receives Ubiquitin from a Ubiquitin-activating (E1) enzyme and subsequently interacts with a Ubiquitin ligase (E3) to conjugate Ubiquitin to substrate proteins. Reaction conditions will need to be optimized for each specific application. We recommend an initial Recombinant Human UbcH5c/UBE2D3 concentration of 0.1-1 µM. Formulation:Supplied as a solution in HEPES, NaCl, DTT and Glycerol.See Certificate of Analysis for details. Molecule Information: UbcH5c/UBE2D3 Aliases: UBC5C; UBE2D3 Entrez Gene IDs: 7323 (Human); 66105 (Mouse); 81920 (Rat) Background: Ubch5c Ubiquitin-conjugating Enzyme H5c (UbcH5c), also known as Ubiquitin-conjugating Enzyme E2D 3 (UBE2D3), is a member of the yeast Ubc4/5 family of Ubiquitin-conjugating (E2) enzymes. Human UbcH5c/UBE2D3 has a predicted molecular weight of 17 kDa and shares 88% and 89% amino acid sequence identity with the related family members, UbcH5a and UbcH5b, respectively. In combination with Ubiquitin ligases (E3s) such as CHIP, UbcH5c/UBE2D3 mediates the ubiquitination and subsequent degradation of several regulatory proteins. For instance, UbcH5c/UBE2D3 is involved in the poly-ubiquitination and proteasome-mediated degradation of the Nuclear Factor kappaB (NF-kappaB) inhibitor, IkappaB-alpha, and is implicated in NF-kappaB-dependent inflammation. UbcH5c/UBE2D3 also mediates the ubiquitination of Histone H2A and PCNA, suggesting that it functions during transcriptional regulation, DNA replication, and DNA damage responses.