金山科研平台，产品价格货期咨询微信：jinshanbio Source:E. coli-derived, Contains an N-terminal Gly-Ser-Ser and 6-His tag Accession #:P51965 Purity:>95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain. Predicted Molecular Mass:23 kDa Activity:Recombinant Human His6-UbcH6/UBE2E1 is a member of the Ubiquitin-conjugating (E2) enzyme family that receives Ubiquitin from a Ubiquitin-activating (E1) enzyme and subsequently interacts with a Ubiquitin ligase (E3) to conjugate Ubiquitin to substrate proteins. Reaction conditions will need to be optimized for each specific application. We recommend an initial Recombinant Human His6-UbcH6/UBE2E1 concentration of 0.1-1 µM. Formulation:Supplied as a solution in HEPES, NaCl, DTT and Glycerol.See Certificate of Analysis for details. Molecule Information: UbcH6/UBE2E1 Aliases: UBE2E1 Entrez Gene IDs: 7324 (Human); 22194 (Mouse) Background: UbcH6/UBE2E1 Ubiquitin-conjugating Enzyme H6 (UbcH6), also known as Ubiquitin-conjugating Enzyme E2E 1 (UBE2E1), is a ubiquitously expressed protein with a predicted molecular weight of 21 kDa. Human UbcH6/UBE2E1 shares 99% and 100% amino acid sequence identity with mouse and rat UBE2E1, respectively. UbcH6/UBE2E1 mediates the selective degradation of short-lived and abnormal proteins. UbcH6/UBE2E1 catalytic activity is modulated following the addition of the Ubiquitin-like protein ISG15 to Lys136. In the nucleus, the N-terminal fragment of UbcH6/UBE2E1 interacts with the Ubiquitin ligase (E3) TRIM21 to mediate substrate ubiquitination. UbcH6/UBE2E1 also interacts with the viral E3, ICP0, during HSV-1 infection. In addition, UbcH6/UBE2E1-dependent protein ubiquitination is implicated in polyglutamine tract disorders such as ataxia.