金山科研平台，产品价格货期咨询微信：jinshanbio Source:E. coli-derived, Contains an N-terminal Met and 7-His tag Accession #:O00762 Purity:>95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain. Predicted Molecular Mass:21 kDa Activity:Recombinant Human His6-UbcH10/UBE2C is a member of the Ubiquitin-conjugating (E2) enzyme family that receives Ubiquitin from a Ubiquitin-activating (E1) enzyme and subsequently interacts with a Ubiquitin ligase (E3) to conjugate Ubiquitin to substrate proteins. Reaction conditions will need to be optimized for each specific application. We recommend an initial Recombinant Human His6-UbcH10/UBE2C concentration of 0.1-1 µM. Formulation:Supplied as a solution in HEPES, NaCl, DTT and Glycerol.See Certificate of Analysis for details. Molecule Information: UbcH10/UBE2C Aliases: UBE2C Entrez Gene IDs: 11065 (Human); 68612 (Mouse); 296368 (Rat) Background: UbcH10/UBE2C Ubiquitin-conjugating Enzyme H10 (UbcH10), also known as Ubiquitin-conjugating Enzyme E2C (UBE2C), is a 179 amino acid (aa) member of the yeast Ubc4/5 family of Ubiquitin-conjugating (E2) enzymes and has a predicted molecular weight of 20 kDa. Human UbcH10/UBE2C shares 96% aa sequence identity with mouse and rat UBE2C. UbcH10/UBE2C is an essential mediator of mitotic destruction events and cell cycle progression. UbcH10/UBE2C recognizes TEK sequences in target proteins such as Cyclins A and B, mediates Lys11-linked ubiquitination, and promotes target protein degradation in conjunction with APC/C, a Ubiquitin ligase (E3). The catalytic activity of UbcH10/UBE2C is regulated by a conserved N-terminal extension, which mediates E2-E3 interaction. UbcH10/UBE2C is overexpressed in a variety of human cancers, and alternate splice isoforms may contribute to uncontrolled cell proliferation and tumor progression.