货号：AB3080P

品牌：Merck Millipore

规格：50Ug

目录价：￥6127.00

市场价格：￥5207.95

会员价格：￥4901.60

金山科研平台，产品价格货期咨询微信：jinshanbio Description: Anti-Green Fluorescent Protein Antibody | AB3080P View All» Promotional Text: Special Shipping Offer on Antibodies100% Performance Guaranteed View All» Trade Name: Chemicon (Millipore) View All» Specificity: AB3080P is made against highly-purified native GFP from Aequorea victoria. It is reactive with GFP from both native and recombinant sources. While other commercial antibodies to GFP typically demonstrate strong reactivity with E. coli, the AB3080P GFP antibody has been affinity purified, followed by extensive adsorption against whole E. coli BL21 strain cells, giving them minimal cross-reactivity with E. coli. View All» Molecular Weight: Varies (GFP is 27 kDa) View All» Immunogen: Highly purified native GFP from Aequorea victoria. View All» Background Information: The Green Fluorescent Protein (GFP) from the jellyfish Aequorea victoria is used as a fluorescent indicator for monitoring gene expression in a variety of cellular systems, including living organisms and fixed tissues. Unlike other bioluminescent reporters, GFP fluoresces in the absence of substrates, cofactors, or other intrinsic or extrinsic proteins. Purified GFP is a 27 kDa monomer consisting of 238 amino acids and emits green light (emission maximum at 509 nm) when excited with blue or UV light. View All» Species Reactivity:

• Vertebrates

• Rat
  View All» Species Reactivity Note: Most common vertebrate species tested. Reactivity with other species has not been confirmed. Expected to react with rat based on sequence homology. View All» Application Notes: Western Blot: A 1:500-1:1000 dilution of a previous lot was used using colorimetric detection (Peroxidase/TMB), with higher dilution possible using more sensitive detection methods. ELISA: A 1:800-1:1,000 dilution of a previous lot was used in ELISA.Note: While AB3080 has been adsorbed against E. coli proteins, most secondary antibodies have not. To reduce the likelihood of E. coli reactivity from a secondary antibody, we suggest the use of Millipore's highly purified secondary antibodies in indirect detection systems.Immunocytochemistry:Optimal working dilutions and protocols must be determined by end user. View All» Control:
  • Cells transfected with GFP-tagged fusion vector

  • RENCX cells
    View All» Quality Assurance: Routinely evaluated by immunocytochemistry on RENCX cells.Immunocytochemistry Analysis: Confocal fluorescent analysis of RENCX constitutively expressed GFP using this lot's anti-GFP Rabbit pAB (Red). Green indicates the expressed GFP. View All» Purification Method: Affinity purified rabbit immunoglobulin View All» Presentation: Affinity purified rabbit polyclonal IgG in 10 mM Tris buffer. View All» Storage Conditions: Stable for 1 year at -20°C in undiluted aliquots from date of receipt.Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. View All» UniProt Number: P42212 View All» Gene Symbol:
    • GFP

    • eGFP
      View All» Alternate Names: GFP; eGFP View All» Usage Statement: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. View All» Key Applications:
      • ELISA

      • Immunocytochemistry

      • Western Blotting
        View All» Brand Family: Chemicon View All» Product Name: Anti-Green Fluorescent Protein Antibody | AB3080P View All» Concentration: 1.0 mg/mL View All» Antibody Type: Polyclonal Antibody View All» Qty/Pk: 50 µg View All» Format: Affinity Purified View All» Host: Rabbit View All»
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