货号：AB3150

品牌：Merck Millipore

规格：100UL

目录价：￥5666.00

市场价格：￥4816.10

会员价格：￥4532.80

金山科研平台，产品价格货期咨询微信：jinshanbio Description: Anti-Fractin Antibody, C-terminus | AB3150 View All» Promotional Text: Special Shipping Offer on Antibodies100% Performance Guaranteed View All» Trade Name: Chemicon (Millipore) View All» Specificity: By immunohistochemistry and Western blot, this antibody only recognizes the 32 kDa Fractin fragment but not full-length beta actin. View All» Epitope: C-terminus View All» Immunogen: K-YELPD peptide representing the last five amino acids of the C-terminus of the 32 kDa Fractin fragment of beta-Actin. This sequence is absolutely conserved in Fractin fragments from human, mouse, rat, feline, porcine, ovine, bovine, canine, rabbit and guinea pig origin. View All» Background Information: During apoptosis, beta actin is cleaved by activated caspase 3 between Asp244 and Gly245. This cleavage results in the release of an approximately 130 amino acid (15 kDa) fragment from the C-terminus of beta actin and a 32 kDa (244 amino acid) N-terminal fragment of actin, known as Fractin. The C-terminus of the Fractin fragment represents an apoptosis-specific neoepitope that is not antigenic in native full-length beta actin and therefore presents a novel and specific marker for distinguishing apoptotic from necrotic cells. View All» Species Reactivity:

• Human

• Mouse

• Rat
  View All» Application Notes: Immunohistochemistry/Immunocytochemistry: 1:400-1:1000. 1 hr at 37°C or 4°C overnight. Note: If studying paraffin-embedded tissue, the use of antigen retrieval methods is recommended. Western Blot: 1:1000-1:4000 for incubation overnight at 4°C. Recommended antibody dilution buffer: TBS with 3% BSA and 0.05% Tween 20, pH 7.4 Optimal working dilutions must be determined by end user. View All» Control: POSITIVE CONTROL: Staurosporin treated neuronal cultures. View All» Presentation: Liquid whole rabbit serum containing no preservatives. View All» Storage Conditions: Aliquot and store at -20°C for up to 12 months from date of receipt. Avoid repeated freeze-thaw cycles. View All» UniProt Number: P60709 View All» Entrez Gene Number: NM_001101.2 View All» Gene Symbol:
  • ACTB

  • PS1TP5BP1

  • beta-actin

  • Beta-actin
    View All» Alternate Names: 32 kDa Fragment of beta-Actin View All» Usage Statement: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. View All» Key Applications:
    • Western Blotting

    • Immunocytochemistry

    • Immunohistochemistry (Paraffin)
      View All» Entrez Gene Summary: This gene encodes one of six different actin proteins. Actins are highly conserved proteins that are involved in cell motility, structure, and integrity. This actin is a major constituent of the contractile apparatus and one of the two nonmuscle cytoskeletal actins. View All» UniProt Summary: FUNCTION: SwissProt: P60709 # Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.SIZE: 375 amino acids; 41737 Da SUBUNIT: Polymerization of globular actin (G-actin) leads to a structural filament (F-actin) in the form of a two-stranded helix. Each actin can bind to 4 others. Found in a complex with XPO6, Ran, ACTB and PFN1. Component of a complex composed at least of ACTB, AP2M1, AP2A1, AP2A2, MEGF10 and VIM. Interacts with XPO6.SUBCELLULAR LOCATION: Cytoplasm, cytoskeleton.DISEASE: SwissProt: P60709 # Defects in ACTB are a cause of juvenile-onset dystonia [MIM:607371]. Dystonia is a movement disorder with a neurological basis, due to disordered tonicity of muscle. It is characterized by sustained involuntary muscle contractions that cause abnormal postures and repetitive movements. It may affect muscles throughout the body (generalized), in certain parts of the body (segmental), or may be confined to particular muscles or muscle groups (focal). Individuals with juvenile-onset dystonia due to ACTB mutations present a complicated phenotype that includes progressive generalized dopa-unresponsive dystonia, developmental malformations and sensory hearing loss.SIMILARITY: SwissProt: P60709 ## Belongs to the actin family.MISCELLANEOUS: In vertebrates 3 main groups of actin isoforms, alpha, beta and gamma have been identified. The alpha actins are found in muscle tissues and are a major constituent of the contractile apparatus. The beta and gamma actins coexist in most cell types as components of the cytoskeleton and as mediators of internal cell motility. View All» Brand Family: Chemicon View All» Product Name: Anti-Fractin Antibody, C-terminus | AB3150 View All» Antibody Type: Polyclonal Antibody View All» Qty/Pk: 100 µL View All» Format: Serum View All» Host: Rabbit View All»
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