货号：AB3505

品牌：Merck Millipore

规格：

目录价：￥3522.00

市场价格：￥2993.70

会员价格：￥2817.60

金山科研平台，产品价格货期咨询微信：jinshanbio Description: Anti-PDX-1 Antibody | AB3505 View All» Replaces: AB3243 View All» Promotional Text: Special Shipping Offer on Antibodies100% Performance Guaranteed View All» Trade Name: Chemicon (Millipore) View All» Specificity: PDX-1 (Pancreas duodenum homeobox-1). The antibody reacts with both natural and recombinant protein. Epitope location is cytoplasmic and nuclear. View All» Immunogen: Synthetic peptide from the c-terminus of mouse PDX-1. View All» Species Reactivity:

• Human

• Mouse
  View All» Application Notes: Western blot: 1:1,000-1:2,000 using ECL. The antibody reacts a protein of 30-40 kDa. Suggested blocking buffer is TBS containing 5% non-fat milk. Suggested gel percentage is 9%. Immunohistochemistry: 1:200-1:500 on frozen and paraffin embedded tissues sections fixed with 4% paraformaldehyde. Suggested permeablization solution is alcohol. Blocking buffer should contain 5% nonfat milk and 5% horse serum. Suggested antigen retrieval solution is citrate buffer. Immunoprecipitation: 5 μL/100 μL. Final reaction volume: 105 μL. Final total protein concentration in reaction mix: 1 mg/mL. Suggested capture agent is Protein A beads. ELISA, indirect: 1:36,000Optimal working dilutions must be determined by the end user. View All» Control: POSITIVE CONTROL: Pancreas. View All» Presentation: Rabbit serum. Liquid. Contains no preservative. View All» Storage Conditions: Maintain at -20°C in undiluted aliquots for up to 12 months after date of receipt. Avoid repeated freeze/thaw cycles. View All» UniProt Number: P52945 View All» Entrez Gene Number: NM_000209.2 View All» Gene Symbol:
  • PDX1

  • IPF1

  • IDX-1

  • GSF

  • IUF1

  • IPF-1

  • STF-1

  • MODY4

  • PDX-1

  • IUF-1
    View All» Alternate Names: Pancreas Duodenum Homeobox-1 View All» Usage Statement: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. View All» Key Applications:
    • ELISA

    • Immunoprecipitation

    • Western Blotting

    • Immunohistochemistry
      View All» Entrez Gene Summary: Insulin promoter factor 1 is an islet-specific protein that activates transcription of the insulin andsomatostatin genes. It is a key regulator of islet peptide hormone expressionn and also plays an essential role in pancreatic development. Mutations in this gene may be involved in several disorders of the pancreas or in diabetes mellitus. View All» UniProt Summary: FUNCTION: SwissProt: P52945 # Activates insulin, somatostatin, glucokinase, islet amyloid polypeptide and glucose transporter type 2 gene transcription. Particularly involved in glucose-dependent regulation of insulin gene transcription. Binds preferentially the DNA motif 5'-[CT]TAAT[TG]-3'. During development, specifies the early pancreatic epithelium, permitting its proliferation, branching and subsequent differentiation. At adult stage, required for maintaining the hormone-producing phenotype of the beta-cell.SIZE: 283 amino acids; 30771 Da SUBUNIT: Interacts with the basic helix-loop-helix domains of TCF3(E47) and NEUROD1 and with HMG-I(Y). Interacts with SPOP (By similarity).SUBCELLULAR LOCATION: Nucleus.TISSUE SPECIFICITY: Duodenum and pancreas (Langerhans islet beta cells and small subsets of endocrine non-beta-cells, at low levels in acinar cells).DOMAIN: SwissProt: P52945 The Antp-type hexapeptide mediates heterodimerization with PBX on a regulatory element of the somatostatin promoter (By similarity). & The homeodomain, which contains the nuclear localization signal, not only mediates DNA-binding, but also acts as a protein- protein interaction domain for TCF3(E47), NEUROD1 and HMG-I(Y) (By similarity).PTM: Phosphorylated by the SAPK2 pathway at high intracellular glucose concentration.DISEASE: SwissProt: P52945 # Defects in PDX1 are a cause of pancreatic agenesis [MIM:260370]. This autosomal recessive disorder is characterized by absence or hypoplasia of pancreas, leading to early-onset insulin-dependent diabetes mellitus. This was found in a frameshift mutation that produces a truncated protein and results in a second initiation that produces a second protein that act as a dominant negative mutant. & Defects in PDX1 are the cause of maturity onset diabetes noninsulin-dependent diabetes mellitus (NIDDM) [MIM:125853]; also known as diabetes mellitus type II. & Defects in PDX1 are the cause of maturity onset diabetes of the young type 4 (MODY4) [MIM:606392]; also symbolized MODY-4. MODY [MIM:606391] is a form of diabetes mellitus characterized by an autosomal dominant mode of inheritance, age of onset of 25 years or younger and a primary defect in insulin secretion.SIMILARITY: Belongs to the Antp homeobox family. IPF1/XlHbox-8 subfamily. & Contains 1 homeobox DNA-binding domain. View All» Brand Family: Chemicon View All» Product Name: Anti-PDX-1 Antibody | AB3505 View All» Antibody Type: Polyclonal Antibody View All» Qty/Pk: 100 µL View All» Format: Serum View All» Host: Rabbit View All»
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