货号：AB3565

品牌：Merck Millipore

规格：100UL

目录价：￥6686.00

市场价格：￥5683.10

会员价格：￥5348.80

金山科研平台，产品价格货期咨询微信：jinshanbio Description: Anti-PARP (214/215) cleavage site Antibody | AB3565 View All» Promotional Text: Special Shipping Offer on Antibodies100% Performance Guaranteed View All» Trade Name: Chemicon (Millipore) View All» Specificity: Human and mouse (86% conserved) PARP. Hamster and rat (86% conserved) PARP have not been tested, but are expected to react. View All» Immunogen: The antiserum was produced against a chemically synthesized peptide corresponding to the N-terminus of cleavage site (214/215) of human PARP. View All» Background Information: Poly (ADP-Ribose) Polymerase (PARP) is a 116 kDa nuclear protein which is strongly activated by binding to DNA strand breaks. PARP plays a role in DNA repair as well as in other cellular processes, including DNA replication, cell proliferation and differentiation. During apoptosis, ICE family members, such as caspase-3 and -7, cleave PARP to yield an 85 kDa and a 25 kDa fragment. PARP cleavage is considered to be one of the classical characteristics of apoptosis. This antibody specifically recognizes the 85 kDa fragment of cleaved PARP and can be used as a marker for detecting apoptotic cells. View All» Species Reactivity:

• Human

• Mouse
  View All» Species Reactivity Note: Hamster and rat (86% homologous) PARP have not been tested, but are expected to react. View All» Application Notes: For Western blot applications, we recommend using the antibody at a 1:1,000 starting dilution. View All» Control: Jurkat and HeLa cells treated with staurosporine or etoposide (25 µM for 3 hours). View All» Quality Assurance: Purified from rabbit serum by affinity chromatography using a peptide corresponding to the PARP cleavage site. View All» Purification Method: Immunoaffinity purified View All» Presentation: Dulbecco's phosphate buffered saline (without Mg²⁺ and Ca²⁺), pH 7.3 (+/- 0.1), 50% glycerol with 1.0 mg/mL BSA (IgG, protease free) as a carrier. 0.05% sodium azide View All» Storage Conditions: Store at -20°C. We recommend a brief centrifugation before opening to settle vial contents. Then, apportion into working aliquots and store at -20°C. For shipment or short-term storage (up to one week), 2-8°C is sufficient. View All» UniProt Number: P09874 View All» Entrez Gene Number: NM_001618.3 View All» Gene Symbol:
  • PARP1

  • poly(ADP-ribosyl)transferase

  • PARP-1

  • PARP

  • NAD

  • pADPRT-1

  • ADPRT1

  • Poly

  • ADPRT

  • PPOL

  • EC 2.4.2.30
    View All» Alternate Names:
    • Poly [ADP-ribose] polymerase 1

    • PARP-1

    • NAD(+) ADP-ribosyltransferase 1

    • ADPRT 1

    • Poly[ADP-ribose] synthase 1

    • ARTD1
      View All» Usage Statement: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. View All» Key Applications:
      • Western Blotting

      • Immunocytochemistry
        View All» Entrez Gene Summary: This gene encodes a chromatin-associated enzyme, poly(ADP-ribosyl)transferase, which modifies various nuclear proteins by poly(ADP-ribosyl)ation. The modification is dependent on DNA and is involved in the regulation of various important cellular processes such as differentiation, proliferation, and tumor transformation and also in the regulation of the molecular events involved in the recovery of cell from DNA damage. In addition, this enzyme may be the site of mutation in Fanconi anemia, and may participate in the pathophysiology of type I diabetes. View All» UniProt Summary: FUNCTION: SwissProt: P09874 # Involved in the base excision repair (BER) pathway, by catalyzing the poly(ADP-ribosyl)ation of a limited number of acceptor proteins involved in chromatin architecture and in DNA metabolism. This modification follows DNA damages and appears as an obligatory step in a detection/signaling pathway leading to the reparation of DNA strand breaks.SIZE: 1014 amino acids; 113084 Da SUBUNIT: Component of a base excision repair (BER) complex, containing at least XRCC1, PARP2, POLB and LIG3. Homo- and heterodimer with PARP2. Interacts with PARP3, APTX and SRY. The SWAP complex consists of NPM1, NCL, PARP1 and SWAP70. Interacts with ZNF423 (By similarity).SUBCELLULAR LOCATION: Nucleus.PTM: Phosphorylated by PRKDC. Phosphorylated upon DNA damage, probably by ATM or ATR. & Poly-ADP-ribosylated by PARP2.SIMILARITY: SwissProt: P09874 ## Contains 1 BRCT domain. & Contains 1 PARP alpha-helical domain. & Contains 1 PARP catalytic domain. & Contains 2 PARP-type zinc fingers. MISCELLANEOUS: The ADP-D-ribosyl group of NAD(+) is transferred to an acceptor carboxyl group on a histone or the enzyme itself, and further ADP-ribosyl groups are transferred to the 2'-position of the terminal adenosine moiety, building up a polymer with an average chain length of 20-30 units. View All» Brand Family: Chemicon View All» Product Name: Anti-PARP (214/215) cleavage site Antibody | AB3565 View All» Antibody Type: Polyclonal Antibody View All» Qty/Pk: 100 µL View All» Format: Affinity Purified View All» Host: Rabbit View All»
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