货号：AB3768

品牌：Merck Millipore

规格：100Ug

目录价：￥5053.00

市场价格：￥4295.05

会员价格：￥4042.40

金山科研平台，产品价格货期咨询微信：jinshanbio Description: Anti-Rad54L Antibody | AB3768 View All» Promotional Text: Special Shipping Offer on Antibodies100% Performance Guaranteed View All» Trade Name: Chemicon (Millipore) View All» Specificity: Recognizes RAD54L. The molecular weight of the protein is 84,326 Daltons. View All» Immunogen: Synthetic peptide, amino acids 12-27 of RAD51L. View All» Species Reactivity:

• Human

• Mouse
  View All» Application Notes: Immunohistochemistry: 1:200-1:1,000. Immunocytochemistry: 1:200-1:1,000. Optimal working dilutions must be determined by the end user.SUGGESTED PROTOCOL FOR IMMUNOHISTOCHEMISTRY 1. Dissect tissues and freeze on dry ice 2. Cut on a cryostat - 10um slices 3. Dry slides at room temperature for 30-90 min 4. Fix with cold Acetone/Methanol (50/50) 2 min - then either process further or store at -20°C . Alternative is fixation with 4% PFA and treatment with a trypsin solution. (0.05% trypsin , incubation 10 minutes at 37°C, followed by 3 washes with PBS) 5. Let air dry 6. PBS 5min 7. Incubate in 50mM ammonium chloride 30 min 8. PBS 5min 9. Blocking serum 30-45min 10. Primary antibody 90min - dilution 1:100 - 1:600 11. Wash 3 times for 5 min 12. Secondary fluorescent conjug. antibody 30min 13. Wash 3 times for 5 min and mount View All» Presentation: Precipitated antibody in a solution of 50% saturated ammonium sulfate and PBS containing no preservatives. PREPARATION AND USE: To reconstitute the antibody, centrifuge the antibody vial at moderate speed (5,000 rpm) for 5 minutes to pellet the precipitated antibody product. Carefully remove the ammonium sulfate/PBS buffer solution and discard. It is not necessary to remove all of the ammonium sulfate/PBS solution: 10 μL of residual ammonium sulfate solution will not effect the resuspension of the antibody. Do not let the protein pellet dry, as severe loss of antibody reactivity can occur. Resuspend the antibody pellet in any suitable biological buffer, standard PBS or TBS (pH 7.3-7.5) are typical. Volumes required are not critical but it is suggested that the final antibody concentration be between 0.1 mg/mL and 1.0 mg/mL. For example, to achieve a 1 mg/mL concentration with 50 μg of precipitated antibody, the amount of buffer needed would be 50 μL. Carefully add the liquid buffer to the pellet. DO NOT VORTEX. Mix by gentle stirring with a wide pipet tip or gentle finger-tapping. Let the precipitated antibody rehydrate for 1 hour at 4-25C° prior to use. Small particles of precipitated antibody that fail to resuspend are normal. Vials are overfilled to compensate for any losses. View All» Storage Conditions: Maintain unopened vial at -70°C for up to 6 months. Avoid repeated freeze/thaw cycles.The rehydrated antibody solutions can be stored undiluted at 2-8C° for 2 months without any significant loss of activity. Note, the solution is not sterile, thus care should be taken if product is stored at 2-8C°. For storage at -20C°, the addition of an equal volume of glycerol can be used, however, it is recommended that ACS grade or higher glycerol be used, as significant loss of activity can occur if the glycerol used is not of high quality.For freezing, it is recommended that the rehydrated antibody solution be further diluted 1:1 with a 2% BSA (fraction V, highest-grade available) solution made with the rehydration buffer. The resulting 1% BSA/antibody solution can be aliquoted and stored frozen at -70C° for up to 6 months. Avoid repeated freeze/thaw cycles. View All» UniProt Number: Q92698 View All» Entrez Gene Number: NM_003579.2 View All» Gene Symbol:
  • RAD54L

  • HR54

  • hRAD54

  • RAD54A

  • hHR54

  • HRAD54

  • EC 3.6.1.-
    View All» Usage Statement: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. View All» Key Applications:
    • Immunocytochemistry

    • Immunohistochemistry
      View All» Entrez Gene Summary: The protein encoded by this gene belongs to the DEAD-like helicase superfamily, and shares similarity with Saccharomyces cerevisiae Rad54, a protein known to be involved in the homologous recombination and repair of DNA. This protein has been shown to play a role in homologous recombination related repair of DNA double-strand breaks. The binding of this protein to double-strand DNA induces a DNA topological change, which is thought to facilitate homologous DNA paring, and stimulate DNA recombination. View All» UniProt Summary: FUNCTION: SwissProt: Q92698 # Involved in DNA repair and mitotic recombination. Functions in the recombinational DNA repair (RAD52) pathway. Dissociates RAD51 from nucleoprotein filaments formed on dsDNA. Could be involved in the turnover of RAD51 protein-dsDNA filaments (By similarity). May play also an essential role in telomere length maintenance and telomere capping in mammalian cells.SIZE: 747 amino acids; 84352 Da SUBUNIT: Interacts with RAD51 through the NH2-terminal domain.SUBCELLULAR LOCATION: Nucleus.DISEASE:SwissProt: Q92698 # Defects in RAD54L may be a cause of tumor formation. Although alterations are found in a small fraction of primary tumors, these findings provide new insight into genetic instability underlying carcinogenesis.SIMILARITY: SwissProt: Q92698 ## Belongs to the SNF2/RAD54 helicase family. & Contains 1 helicase ATP-binding domain. & Contains 1 helicase C-terminal domain. View All» Brand Family: Chemicon View All» Product Name: Anti-Rad54L Antibody | AB3768 View All» Antibody Type: Polyclonal Antibody View All» Qty/Pk: 100 µg View All» Format: Affinity Purified View All» Host: Rabbit View All»
      © 金山科研平台是专业的授权总代理区域代理经销平台。
      © 如需询价，请加客服QQ：1749072012 、客服微信：jinshanbio，或发送邮件到1749072012@qq.com
      © 平台为生命科学研究相关领域提供一站式耗材试剂仪器解决方案和采购服务，数据资源基于CC协议。
      © 本文地址：https://www.16ao.com/thread-153527.htm
      © Merck Millipore代理AB3768 Anti-Rad54L Antibody;store at -80℃，产品报价联系微信jinshanbio