货号：AB3848

品牌：Merck Millipore

规格：

目录价：￥3978.00

市场价格：￥3381.30

会员价格：￥3182.40

金山科研平台，产品价格货期咨询微信：jinshanbio Description: Anti-phosphoSmad1/Smad5/Smad8 Antibody, phospho-specific (Ser463/465) | AB3848 View All» Promotional Text: Special Shipping Offer on Antibodies100% Performance Guaranteed View All» Trade Name: Chemicon (Millipore) View All» Specificity: Recognizes Smad1 only when dually phosphorylated at Ser463 and Ser465, as well as phosphorylated Smad5 and Smad8 at the equivalent sites. This antibody does not crossreact with other Smad-related proteins. View All» Molecular Weight: 53 kDa View All» Epitope: phospho-specific (Ser463/465) View All» Immunogen: KLH-conjugated, synthetic phospho-peptide corresponding to residues surrounding Ser463/465 of human Smad5. View All» Modifications: Phosphorylation View All» Background Information: Transcriptional modulator activated by BMP (bone morphogenetic proteins) type 1 receptor kinase. SMAD1 is a receptor-regulated SMAD (R-SMAD). View All» Species Reactivity:

• Human

• Mouse

• Rat

• Mink

• Xenopus
  View All» Species Reactivity Note: Human and mouse Expected to cross-react with rat, mink, and Xenopus. Reactivity with other species has not been determined. View All» Application Notes: Immunohistochemistry (Paraffin): 1:100 dilutoin of a previous lot was used; a high heat antigen retrieval technique is suggested using a citrate/TBST buffer.Immunocytochemistry (IF): 1:100 dilution of a previous lot was used.Flow Cytometry: 1:50 dilution of a previous lot was usedWestern blotting: 1:1,000. For best results, incubate membrane with diluted antibody in 5% BSA, 1X TBS, 0.1% Tween-20 at 4°C with gentle shaking, overnight. Optimal working dilutions must be determined by the end user. View All» Control: BMP-4-treated HeLa or NIH/3T3 cells Embryo. View All» Quality Assurance: Routinely evaluated by Western Blot on untreated and BMP2 treated HEK293 lysates.Western Blot Analysis: 1:500 dilution of this lot detected pSmad1/5/8 on 10 μg of BMP2 treated HEK293 lysates. View All» Purification Method: Immunoaffinity purified View All» Presentation: Affinity purified rabbit polyclonal in buffer containing 10 mM sodium HEPES, pH 7.5, 150 mM NaCl, 100 μg/mL BSA, with 50% glycerol. View All» Storage Conditions: Stable for 1 year at -20°C from date of receipt. Do not aliquot antibody.Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variabillity in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage. View All» UniProt Number: Q15797 View All» Entrez Gene Number: NM_001003688.1 View All» Gene Symbol:
  • SMAD1

  • MADR1

  • JV41

  • JV4-1

  • MADH1

  • BSP-1

  • BSP1

  • GARS

  • CMT2D

  • hSMAD1

  • HMN5
    View All» Alternate Names: Mothers against decapentaplegic homolog View All» Usage Statement: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. View All» Key Applications:
    • Flow Cytometry

    • Immunocytochemistry

    • Immunohistochemistry (Paraffin)

    • Western Blotting
      View All» Entrez Gene Summary: The protein encoded by this gene belongs to the SMAD, a family of proteins similar to the gene products of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. This protein mediates the signals of the bone morphogenetic proteins (BMPs), which are involved in a range of biological activities including cell growth, apoptosis, morphogenesis, development and immune responses. In response to BMP ligands, this protein can be phosphorylated and activated by the BMP receptor kinase. The phosphorylated form of this protein forms a complex with SMAD4, which is important for its function in the transcription regulation. This protein is a target for SMAD-specific E3 ubiquitin ligases, such as SMURF1 and SMURF2, and undergoes ubiquitination and proteasome-mediated degradation. Alternatively spliced transcript variants encoding the same protein have been observed View All» UniProt Summary: FUNCTION: SwissProt: Q99717 # Transcriptional modulator activated by BMP (bone morphogenetic proteins) type 1 receptor kinase. SMAD5 is a receptor-regulated SMAD (R-SMAD).SIZE: 465 amino acids; 52258 Da SUBUNIT: May form trimers with the co-SMAD SMAD4. Interacts with PEBP2-alpha subunit and SMURF1. Interacts with SUV39H1 and SUV39H2.SUBCELLULAR LOCATION: Cytoplasm. Nucleus. Note=Cytoplasmic in the absence of ligand. Migrates to the nucleus when complexed with SMAD4.TISSUE SPECIFICITY: Ubiquitous.PTM: Phosphorylated on serine by BMP (bone morphogenetic proteins) type 1 receptor kinase. & Ubiquitin-mediated proteolysis by SMAD-specific E3 ubiquitin ligase SMURF1.SIMILARITY: SwissProt: Q99717 ## Belongs to the dwarfin/SMAD family. & Contains 1 MH1 (MAD homology 1) domain. & Contains 1 MH2 (MAD homology 2) domain View All» Brand Family: Chemicon View All» Product Name: Anti-phosphoSmad1/Smad5/Smad8 Antibody, phospho-specific (Ser463/465) | AB3848 View All» Concentration: Variable View All» Antibody Type: Polyclonal Antibody View All» Qty/Pk: 100 µL View All» Format: Affinity Purified View All» Host: Rabbit View All»
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