货号：AB4132

品牌：Merck Millipore

规格：100Ug

目录价：￥4967.00

市场价格：￥4221.95

会员价格：￥3973.60

金山科研平台，产品价格货期咨询微信：jinshanbio Description: Anti-GATA4 Antibody | AB4132 View All» Promotional Text: Special Shipping Offer on Antibodies100% Performance Guaranteed View All» Trade Name: Chemicon (Millipore) View All» Specificity: Recognizes GATA4. View All» Molecular Weight: 45 kDa View All» Immunogen: Synthetic peptide corresponding to amino acids 313-331 of human GATA4 (RKRKPKNLNKSKTP). View All» Background Information: GATA4 (GATA binding factor-4) is a member of the GATA family of transcription factors. GATA4 is involved in embryogenesis and in myocardial differentiation and function and is associated with heart septal defects. View All» Species Reactivity:

• Human

• Mouse

• Rat
  View All» Application Notes: Western Blot and Immunocytochemistry: 1/200 to 1/1000. Optimal dilutions must be determined by the user. View All» Control: Heart tissue View All» Purification Method: Immunoaffinity purified View All» Presentation: Affinity Purified immunoglobulin precipitated in a solution of 50% saturated ammonium sulfate and PBS containing no preservatives. View All» Storage Conditions: Maintain unopened vial at -20°C for up to 6 months. Avoid repeated freeze/thaw cycles. The rehydrated antibody solutions can be stored undiluted at 2–8°C for 2 months without any significant loss of activity. Note, the solution is not sterile, thus care should be taken if product is stored at 2–8°C. For storage at -20°C, the addition of an equal volume of glycerol can be used, however, it is recommended that ACS grade or higher glycerol be used, as significant loss of activity can occur if the glycerol used is not of high quality. For freezing, it is recommended that the rehydrated antibody solution be further diluted 1:1 with a 2% BSA (fraction V, highest-grade available) solution made with the rehydration buffer. The resulting 1% BSA/antibody solution can be aliquoted and stored frozen at -70°C for up to 6 months. Avoid repeated freeze/thaw cycles. PREPARATION AND USE: To reconstitute the antibody, centrifuge the antibody vial at moderate speed (5,000 rpm) for 5 minutes to pellet the precipitated antibody product. Carefully remove the ammonium sulfate/PBS buffer solution and discard. It is not necessary to remove all of the ammonium sulfate/PBS solution: 10 μL of residual ammonium sulfate solution will not effect the resuspension of the antibody. Do not let the protein pellet dry, as severe loss of antibody reactivity can occur. Resuspend the antibody pellet in any suitable biological buffer, standard PBS or TBS (pH 7.3–7.5) are typical. Volumes required are not critical but it is suggested that the final antibody concentration be between 0.1 mg/mL and 1.0 mg/mL. For example, to achieve a1 mg/mL concentration with 50 μg of precipitated antibody, the amount of buffer needed would be 50 μL. Carefully add the liquid buffer to the pellet. DO NOT VORTEX. Mix by gentle stirring with a wide pipet tip or gentle finger-tapping. Let the precipitated antibody rehydrate for 1 hour at 4–25°C prior to use. Small particles of precipitated antibody that fail to resuspend are normal. Vials are overfilled to compensate for any losses. View All» UniProt Number: P43694 View All» Entrez Gene Number: NM_002052.2 View All» Gene Symbol:
  • GATA4

  • MGC126629
    View All» Usage Statement: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. View All» Key Applications:
    • Immunocytochemistry

    • Western Blotting
      View All» Entrez Gene Summary: This gene encodes a member of the GATA family of zinc-finger transcription factors. Members of this family recognize the GATA motif which is present in the promoters of many genes. This protein is thought to regulate genes involved in embryogenesis and in myocardial differentiation and function. Mutations in this gene have been associated with cardiac septal defects. View All» UniProt Summary: FUNCTION: SwissProt: P43694 # Transcriptional activator. Binds to the consensus sequence 5'-AGATAG-3'. Acts as a transcriptional activator of ANF in cooperation with NKX2-5 (By similarity).SIZE: 442 amino acids; 44627 Da SUBUNIT: Interacts with ZNF260 (By similarity). Interacts with the homeobox domain of NKX2-5 through its C-terminal zinc finger. Also interacts with JARID2 which represses its ability to activate transcription of ANF (By similarity).SUBCELLULAR LOCATION: Nucleus.DISEASE: SwissProt: P43694 # Defects in GATA4 are a cause of atrial septal defect 2 (ASD2) [MIM:607941]. ASD2 is an autosomal dominant condition with atrial septal defect and other congenital heart disease but no conduction defects or non-cardiac abnormalities.SIMILARITY: SwissProt: P43694 ## Contains 2 GATA-type zinc fingers. View All» Brand Family: Chemicon View All» Product Name: Anti-GATA4 Antibody | AB4132 View All» Antibody Type: Polyclonal Antibody View All» Qty/Pk: 100 μg View All» Format: Affinity Purified View All» Host: Rabbit View All»
      © 金山科研平台是专业的授权总代理区域代理经销平台。
      © 如需询价，请加客服QQ：1749072012 、客服微信：jinshanbio，或发送邮件到1749072012@qq.com
      © 平台为生命科学研究相关领域提供一站式耗材试剂仪器解决方案和采购服务，数据资源基于CC协议。
      © 本文地址：https://www.16ao.com/thread-153654.htm
      © Merck Millipore代理AB4132 Anti-GATA4 Antibody;store at -20℃，产品报价联系微信jinshanbio