货号：AB5158-50UL

品牌：Merck Millipore

规格：50UL

目录价：￥4452.00

市场价格：￥3784.20

会员价格：￥3561.60

金山科研平台，产品价格货期咨询微信：jinshanbio Description: Anti-Calcium Channel Antibody, Voltage Gated α 1D | AB5158-50UL View All» Promotional Text: Special Shipping Offer on Antibodies100% Performance Guaranteed View All» Trade Name: Chemicon (Millipore) View All» Specificity: Recognizes all forms of a Cav1.3 (alpha 1D subunit) from the voltage-gated calcium channel. Does not cross react with any other calcium channel antigens tested so far. Sequence conservation: hamster and mouse (16/17); human (15/17); chicken (12/17) residues identical. View All» Immunogen: Purified peptide CND1 from 859-875 of alpha 1D subunit of rat brain voltage-gated calcium channel (Accession P27732). View All» Background Information: Voltage-sensitive calcium channels (vscc) mediate the entry of calcium ions into excitable cells and are also involved in a variety of calcium-dependent processes, including muscle contraction, hormone or neurotransmitter release, gene expression, cell motility, cell division and cell death. The isoform alpha-1c gives rise to l-type calcium currents. Long-lasting (l-type) calcium channels belong to the "high-voltage activated" (hva) group. They are blocked by dihydropyridines (dhp), phenylalkylamines, benzothiazepines, and by omega-agatoxin-IIIA (omega-aga-IIIA). They are however insensitive to omega-conotoxin- gvia (omega-ctx-gvia) and omega-agatoxin-IVA (omega-aga-IVA) (Swiss Prot). View All» Species Reactivity:

• Mouse

• Rat
  View All» Application Notes: In preparing full-length protein samples for Western blot or immunoprecipitation, the procedures should be performed at 4°C, with the following protease inhibitor mixture: pepstatin A (1 μg/mL), leupeptin (1 μg/mL), aprotinin (1 μg/mL), Pefabloc SC (0.2 mM), benzamidine (0.1 mg/mL), and calpain inhibitors I and II (8 μg/mL each). Western blot: 1:200 using ECL on rat membrane preperations. LMW form shows a 190kDa band while the HMW form shows a 210kDa band. Immunohistochemistry on rat brain sections. Dilutions should be made using a carrier protein such as BSA (1-3%). Optimal working dilutions and protocols must be determined by the end user. View All» Control: CONTROL ANTIGEN: Included free of charge with the antibody is 20 μg of control antigen (lyophilized powder in phosphate buffered saline, pH 7.4, containing 5% sucrose and 0.025% sodium azide). The stock solution of the antigen can be made up using 100 μL of sterile deionized water. For negative control, preincubate 0.5-1 μg of peptide with 1 μg of antibody for one hour at room temperature. Optimal concentrations must be determined by the end user. View All» Presentation: Affinity purified immunoglobulin. Lyophilized from phosphate buffered saline, pH 7.4, containing 1% BSA, 5% sucrose as a stabilizer and 0.05% sodium azide as a preservative. Reconstitute with 50 μL of sterile deionized water. Centrifuge antibody preparation before use (10,000 xg for 5 min). View All» Storage Conditions: Maintain lyophilized material at -20°C for up to 12 months after date of receipt. After reconstitution maintain at -20°C in undiluted aliquots for up to 6 months. Avoid repeated freeze/thaw cycles. View All» UniProt Number: Q01668 View All» Entrez Gene Number: NM_000720.1 View All» Gene Symbol:
  • CACNA1D

  • CCHL1A2

  • CACH3

  • Cav1.3

  • CACN4

  • CACNL1A2
    View All» Alternate Names: L-type View All» Usage Statement: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. View All» Key Applications:
    • Immunohistochemistry

    • Immunoprecipitation

    • Western Blotting
      View All» UniProt Summary: FUNCTION: SwissProt: Q01668 # Voltage-sensitive calcium channels (VSCC) mediate the entry of calcium ions into excitable cells and are also involved in a variety of calcium-dependent processes, including muscle contraction, hormone or neurotransmitter release, gene expression, cell motility, cell division and cell death. The isoform alpha-1D gives rise to L-type calcium currents. Long-lasting (L-type) calcium channels belong to the high-voltage activated (HVA) group. They are blocked by dihydropyridines (DHP), phenylalkylamines, benzothiazepines, and by omega-agatoxin-IIIA (omega-Aga-IIIA). They are however insensitive to omega-conotoxin- GVIA (omega-CTx-GVIA) and omega-agatoxin-IVA (omega-Aga-IVA).SIZE: 2161 amino acids; 245141 Da SUBUNIT: Voltage-dependent calcium channels are multisubunit complexes, consisting of alpha-1, alpha-2, beta and delta subunits in a 1:1:1:1 ratio. The channel activity is directed by the pore- forming and voltage-sensitive alpha-1 subunit. In many cases, this subunit is sufficient to generate voltage-sensitive calcium channel activity. The auxiliary subunits beta and alpha-2/delta linked by a disulfide bridge regulate the channel activity. Interacts with RIMBP2 (By similarity).SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein.TISSUE SPECIFICITY: Expressed in pancreatic islets and in brain, where it has been seen in hippocampus, basal ganglia, habenula and thalamus. No expression in skeletal muscle.DOMAIN: SwissProt: Q01668 Each of the four internal repeats contains five hydrophobic transmembrane segments (S1, S2, S3, S5, S6) and one positively charged transmembrane segment (S4). S4 segments probably represent the voltage-sensor and are characterized by a series of positively charged amino acids at every third position.SIMILARITY: Belongs to the calcium channel alpha-1 subunit family. View All» Brand Family: Chemicon View All» Product Name: Anti-Calcium Channel Antibody, Voltage Gated α 1D | AB5158-50UL View All» Concentration: 0.7 mg/mL (after reconstitution) View All» Antibody Type: Polyclonal Antibody View All» Qty/Pk: 50 µL View All» Format: Affinity Purified View All» Host: Rabbit View All»
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