货号：AB5188-50UL

品牌：Merck Millipore

规格：50UL

目录价：￥4754.67

市场价格：￥4041.47

会员价格：￥3803.74

金山科研平台，产品价格货期咨询微信：jinshanbio Description: Anti-Potassium Channel Kv3.1b Antibody | AB5188-50UL View All» Promotional Text: Special Shipping Offer on Antibodies100% Performance Guaranteed View All» Trade Name: Chemicon (Millipore) View All» Specificity: Recognizes a full length Kv3.1b protein. Does not cross react with any other potassium channel antigens tested so far. View All» Immunogen: Purified peptide from rat Kv3.1b (amino acids 567-585) (Accession P251222), conjugated to KLH. View All» Species Reactivity:

• Mouse

• Rat
  View All» Application Notes: Immunohistochemistry on rat brain sections (Hartig et al. 1999; Atkinson & Deuchars 2000) Western blot: 1:200 using ECL Immunoprecipitation (Sobko et al. 1998a,b) Dilutions should be made using a carrier protein such as BSA (1-3%) Optimal working dilutions must be determined by the end user. SUGGESTED PROTOCOL FOR IMMUNOHISTOCHEMISTRY Sacrifice and tissue processing: Rats are deeply anesthetized with pentobarbital sodium (Pental). Brain is fixed by transcardial perfusion, first with 50 mL of phosphate buffered saline (0.02M PBS, pH 7.4) containing heparin (5U/ml), then with 220 mL of ice-cold 4% paraformaldehyde in 0.1M PBS, pH 7.5 containing sucrose 4%. Brain is cut in coronal blocks and further fixed by immersion in the same fixative, refrigerated, for 1-2 hours. Brain blocks are transferred to 10% sucrose in 0.1M PBS and sectioned in a cryostat within 3 days. Brain sections, 30 μm thick, are floated in 0.1M PBS and then preserved in a cryopreservation buffer at -20°C. The cryopreservation buffer contains 40% ethylene glycol and 1% polyvinylpyrrolidone in 0.1M potassium acetate buffer, pH 6.5. Staining procedure: 1. Floating sections are rinsed in 0.02M PBS, 2x 5 minutes. 2. Endogenous peroxidase activity is quenched by incubation with 0.2 % hydrogen peroxide in 0.1M phosphate buffer pH 7.3 containing 0.2% Triton X-100 for 25 minutes at room temperature. 3. Sections are rinsed in 0.02M PBS, 2x 5 minutes. 4. Sections are incubated with the primary antiserum in a medium containing 0.3% Triton X-100, 0.05% Tween 20, 4% normal donkey serum (NDS), for 1 hour at room temperature and then overnight refrigerated. 5. Sections are rinsed in 0.02M PBS, containing 4% NDS, 2x 5 minutes. Sections are incubated with biotinylated donkey anti-rabbit (from Chemicon USA, catalog number AP182B) diluted 1:400 in 0.02M PBS, containing 0.3% Triton X-100, 0.05% Tween 20, and 4% NDS, for 1 hour at room temperature and then overnight refrigerated. 1. Sections are rinsed in 0.02M PBS containing 4% NDS, 2x 5 min. 2. Sections are incubated with extravidin-peroxidase (Sigma Catalog number E2886) diluted 1:100 in 0.02M PBS, for 45 minutes at room temperature. 3. Sections are rinsed in 0.02M PBS, 3x 5 minutes. Color development: 1. Sections are incubated with a solution of diaminobenzidine at the concentration of 0.0125% and containing 0.05% nickel ammonium sulfate for 10 minutes at room temperature. 2. Sections are transferred to the same DAB solution but with added hydrogen peroxide at a final concentration of 0.0015%. Duration of incubation should be adjusted by the end user. 3. Sections are rinsed in 0.02M PBS, 4x 10 minutes. 4. Sections are mounted on glass slides (gelatinized or coated by any other type of adhesive material) and allowed to dry. 5. Sections are dehydrated in ascending series of ethanol concentrations (70%, 90%, 100%, 5 minutes in each), delipidated in xylene (10 minutes) and coverslipped in Permount (or any other xylene diluted adhesive). View All» Control: CONTROL ANTIGEN: Included free of charge with the antibody is 20 μg of control antigen (lyophilized powder in phosphate buffered saline, pH 7.4, containing 5% sucrose and 0.025% sodium azide). The stock solution of the antigen can be made up using 200 μL of sterile deionized water. For negative control, preincubate 1 μg of peptide with 1 μg of antibody for one hour at room temperature. Optimal concentrations must be determined by the end user. View All» Presentation: Affinity purified immunoglobulin. Lyophilized from phosphate buffered saline, pH 7.4, containing 1% BSA, 5% sucrose as a stabilizer and 0.025% sodium azide as a preservative. Reconstitute with 50 μL of sterile deionized water. Centrifuge antibody preparation before use (10,000 xg for 5 min). View All» Storage Conditions: Maintain lyophilized material at -20°C for up to 12 months. After reconstitution maintain at -20°C in undiluted aliquots for up to 6 months. Avoid repeated freeze/thaw cycles. View All» UniProt Number: P48547 View All» Entrez Gene Number: NM_004976.3 View All» Gene Symbol:
  • KCNC1

  • KV4

  • Kv3.1

  • NGK2

  • Kv4

  • KV3.1

  • MGC129855
    View All» Usage Statement: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. View All» Key Applications:
    • Immunohistochemistry

    • Immunoprecipitation

    • Western Blotting
      View All» Entrez Gene Summary: The Shaker gene family of Drosophila encodes components of voltage-gated potassium channels and is comprised of four subfamilies. Based on sequence similarity, this gene is similar to one of these subfamilies, namely the Shaw subfamily. The protein encoded by this gene belongs to the delayed rectifier class of channel proteins and is an integral membrane protein that mediates the voltage-dependent potassium ion permeability of excitable membranes. Multiple transcript variants encoding different isoforms have been inferred for this gene based on orthologous loci. View All» UniProt Summary: FUNCTION: SwissProt: P48547 # Mediates the voltage-dependent potassium ion permeability of excitable membranes. Assuming opened or closed conformations in response to the voltage difference across the membrane, the protein forms a potassium-selective channel through which potassium ions may pass in accordance with their electrochemical gradient.SIZE: 511 amino acids; 57942 Da SUBUNIT: Heteromultimer with KCNG3, KCNG4 and KCNV2.SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein.DOMAIN: SwissProt: P48547 The segment S4 is probably the voltage-sensor and is characterized by a series of positively charged amino acids at every third position. & The tail may be important in modulation of channel activity and/or targeting of the channel to specific subcellular compartments.SIMILARITY: Belongs to the potassium channel family. C (Shaw) subfamily. View All» Brand Family: Chemicon View All» Product Name: Anti-Potassium Channel Kv3.1b Antibody | AB5188-50UL View All» Concentration: 15 μg/50 μL (after reconstitution) View All» Antibody Type: Polyclonal Antibody View All» Qty/Pk: 50 µL View All» Format: Affinity Purified View All» Host: Rabbit View All»
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