货号：AB5194-200UL

品牌：Merck Millipore

规格：

目录价：￥8483.00

市场价格：￥7210.55

会员价格：￥6786.40

金山科研平台，产品价格货期咨询微信：jinshanbio Description: Anti-Potassium Channel Kv4.3 Antibody | AB5194-200UL View All» Promotional Text: Special Shipping Offer on Antibodies100% Performance Guaranteed View All» Trade Name: Chemicon (Millipore) View All» Specificity: Recognizes a full length Kv4.3protein. Does not cross react with any other potassium channel antigens tested so far. SPECIES REACTIVITIES: It is expected that the antibody will react with rabbit (17/17) and mouse (16/17) due to sequence homology. Other species have not been tested. View All» Immunogen: Purified peptide from rat Kv4.3 (amino acids 451-467). View All» Species Reactivity: Rat View All» Application Notes: Immunohistochemistry on rat brain sections. Western blot: 1:200 using ECL Dilutions should be made using a carrier protein such as BSA (1-3%) Optimal working dilutions must be determined by the end user. PROTOCOL FOR IMMUNOHISTOCHEMISTRY Sacrifice and tissue processing: Rats are deeply anesthetized with pentobarbital sodium (Pental). Brain is fixed by transcardial perfusion, first with 50 mL of phosphate buffered saline (0.02M PBS, pH 7.4) containing heparin (5U/ml), then with 220 mL of ice-cold 4% paraformaldehyde in 0.1M PBS, pH 7.5 containing sucrose 4%. Brain is cut in coronal blocks and further fixed by immersion in the same fixative, refrigerated, for 1-2 hours. Brain blocks are transferred to 10% sucrose in 0.1M PBS and sectioned in a cryostat within 3 days. Brain sections, 30 μm thick, are floated in 0.1M PBS and then preserved in a cryopreservation buffer at -20°C. The cryopreservation buffer contains 40% ethylene glycol and 1% polyvinylpyrrolidone in 0.1M potassium acetate buffer, pH 6.5. Staining procedure: 1. Floating sections are rinsed in 0.02M PBS, 2x 5 minutes. 2. Endogenous peroxidase activity is quenched by incubation with 0.2 % hydrogen peroxide in 0.1M phosphate buffer pH 7.3 containing 0.2% Triton X-100 for 25 minutes at room temperature. 3. Sections are rinsed in 0.02M PBS, 2x 5 minutes. 4. Sections are incubated with the primary antiserum in a medium containing 0.3% Triton X-100, 0.05% Tween 20, 4% normal donkey serum (NDS), for 1 hour at room temperature and then overnight refrigerated. 5. Sections are rinsed in 0.02M PBS, containing 4% NDS, 2x 5 minutes. Sections are incubated with biotinylated donkey anti-rabbit (from Chemicon USA, catalog number AP182B) diluted 1:400 in 0.02M PBS, containing 0.3% Triton X-100, 0.05% Tween 20, and 4% NDS, for 1 hour at room temperature and then overnight refrigerated. 1. Sections are rinsed in 0.02M PBS containing 4% NDS, 2x 5 min. 2. Sections are incubated with streptavidin-peroxidase, appropriately diluted in 0.02M PBS, for 45 minutes at room temperature. 3. Sections are rinsed in 0.02M PBS, 3x 5 minutes. Color development: 1. Sections are incubated with a solution of diaminobenzidine at the concentration of 0.0125% and containing 0.05% nickel ammonium sulfate for 10 minutes at room temperature. 2. Sections are transferred to the same DAB solution but with added hydrogen peroxide at a final concentration of 0.0015%. Duration of incubation should be adjusted by the end user. 3. Sections are rinsed in 0.02M PBS, 4x 10 minutes. 4. Sections are mounted on glass slides (gelatinized or coated by any other type of adhesive material) and allowed to dry. 5. Sections are dehydrated in ascending series of ethanol concentrations (70%, 90%, 100%, 5 minutes in each), delipidated in xylene (10 minutes) and coverslipped in Permount (or any other xylene diluted adhesive). View All» Control: CONTROL ANTIGEN: Included free of charge with the antibody is 40 μg of control antigen (lyophilized powder in phosphate buffered saline, pH 7.4, containing 5% sucrose and 0.025% sodium azide). The stock solution of the antigen can be made up using 100 μL of sterile deionized water. For negative control, preincubate 1 μg of peptide with 1 μg of antibody for one hour at room temperature. Optimal concentrations must be determined by the end user. View All» Presentation: Affinity purified immunoglobulin. Lyophilized from phosphate buffered saline, pH 7.4, containing 1% BSA, 5% sucrose as a stabilizer and 0.025% sodium azide as a preservative. Reconstitute with 200 μL of sterile deionized water. Centrifuge antibody preparation before use (10,000 xg for 5 min). View All» Storage Conditions: Maintain lyophilized material at -20°C for up to 12 months. After reconstitution maintain at -20°C in undiluted aliquots for up to 6 months. Avoid repeated freeze/thaw cycles. View All» UniProt Number: Q9UK17 View All» Entrez Gene Number:

• NM_004980.3

• NM_172198.1
  View All» Gene Symbol:
  • KCND3

  • OTTHUMP00000063948

  • KV4.3

  • KCND3S

  • KCND3L

  • KSHIVB

  • Kv4.3

  • MGC142037

  • MGC142035
    View All» Usage Statement: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. View All» Key Applications:
    • Immunohistochemistry

    • Western Blotting
      View All» Entrez Gene Summary: Voltage-gated potassium (Kv) channels represent the most complex class of voltage-gated ion channels from both functional and structural standpoints. Their diverse functions include regulating neurotransmitter release, heart rate, insulin secretion, neuronal excitability, epithelial electrolyte transport, smooth muscle contraction, and cell volume. Four sequence-related potassium channel genes - shaker, shaw, shab, and shal - have been identified in Drosophila, and each has been shown to have human homolog(s). This gene encodes a member of the potassium channel, voltage-gated, shal-related subfamily, members of which form voltage-activated A-type potassium ion channels and are prominent in the repolarization phase of the action potential. This member includes two isoforms with different sizes, which are encoded by alternatively spliced transcript variants of this gene. View All» UniProt Summary: FUNCTION: SwissProt: Q9UK17 # Pore-forming (alpha) subunit of voltage-gated rapidly inactivating A-type potassium channels. May contribute to I(To) current in heart and I(Sa) current in neurons. Channel properties are modulated by interactions with other alpha subunits and with regulatory subunits.SIZE: 655 amino acids; 73451 Da SUBUNIT: Homotetramer or heterotetramer with KCND1 and/or KCND2. Associates with the regulatory subunits KCNIP1, KCNIP2, KCNIP3 and KCNIP4 (By similarity). Interacts with KCNE1, KCNE2, SCN1B and KCNAB1.SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein.TISSUE SPECIFICITY: Highly expressed in heart and brain, in particular in cortex, cerebellum, amygdala and caudate nucleus. Detected at lower levels in liver, skeletal muscle, kidney and pancreas. Isoform 1 predominates in most tissues. Isoform 1 and isoform 2 are detected at similar levels in brain, skeletal muscle and pancreas.DOMAIN:SwissProt: Q9UK17 The segment S4 is probably the voltage-sensor and is characterized by a series of positively charged amino acids at every third position.SIMILARITY: Belongs to the potassium channel family. D (Shal) subfamily. View All» Brand Family: Chemicon View All» Product Name: Anti-Potassium Channel Kv4.3 Antibody | AB5194-200UL View All» Concentration: 0.8 mg/mL (after reconstitution) View All» Antibody Type: Polyclonal Antibody View All» Qty/Pk: 200 µL View All» Format: Affinity Purified View All» Host: Rabbit View All»
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