货号：AB5228-200UL

品牌：Merck Millipore

规格：1EA

目录价：￥8390.71

市场价格：￥7132.10

会员价格：￥6712.57

金山科研平台，产品价格货期咨询微信：jinshanbio Description: Anti-Potassium Channel MaxiK Antibody, calcium activated, aa1097-1196 | AB5228-200UL View All» Promotional Text: Special Shipping Offer on Antibodies100% Performance Guaranteed View All» Trade Name: Chemicon (Millipore) View All» Specificity: Corresponds to the C-terminus of the longer form of BK_Ca channel α subunit and overlaps by ~40 residues with a shorter form and not with other known proteins tested so far. View All» Epitope: C-terminal sequence SHSSHSSQ View All» Immunogen: A purified fusion protein (GST) and a C-terminal part (residues 1097-1196) of mouse K_Ca1.1 channel (Accession Q08460-2). View All» Species Reactivity: Rat View All» Application Notes: Western blot: 1:200 using ECL on rat brain membranes.Note: The addition of 0.1% Tween to the standard milk block is recommended.Immunohistochemistry on rat brain sections.Dilutions should be made using a carrier protein such as BSA (1-3%)Optimal working dilutions must be determined by the end user. View All» Control: CONTROL ANTIGEN: Included free of charge with the antibody is 50 μg of control antigen (lyophilized powder in phosphate buffered saline, pH 7.4, containing 5% sucrose and 0.025% sodium azide). The stock solution of the antigen can be made up using 100 μL of sterile deionized water. For positive control, in Western blotting, use 20 ng protein per lane. For negative control, preincubate 3-5 μg of purified peptide with 1 μg of antibody for one hour at room temperature. Optimal concentrations must be determined by the end user. View All» Presentation: Affinity purified immunoglobulin. Lyophilized from phosphate buffered saline, pH 7.4, containing 1% BSA, 5% sucrose as a stabilizer and 0.025% sodium azide as a preservative. Reconstitute with 200 μL of sterile deionized water. Centrifuge antibody preparation before use (10,000 xg five minutes). View All» Storage Conditions: Maintain lyophilized material at -20°C for up to 12 months. After reconstitution maintain at -20°C in undiluted aliquots for up to 6 months. Avoid repeated freeze/thaw cycles. View All» UniProt Number: Q12791 View All» Entrez Gene Number:

• NM_001014797.1

• NM_002247.2
  View All» Gene Symbol:
  • KCNMA1

  • OTTHUMP00000064161

  • OTTHUMP00000060198

  • KCNMA

  • K

  • OTTHUMP00000064158

  • MaxiK

  • VCAlpha)

  • SLO-ALPHA

  • MGC71881

  • OTTHUMP00000064157

  • SAKCA

  • OTTHUMP00000064156

  • OTTHUMP00000064164

  • Slo1

  • BKTM

  • OTTHUMP00000064165

  • hSlo

  • mSLO1

  • OTTHUMP00000064154

  • Slo-alpha

  • SLO

  • OTTHUMP00000064162

  • KCa1.1

  • OTTHUMP00000064155

  • OTTHUMP00000064160

  • DKFZp686K1437

  • OTTHUMP00000064159
    View All» Alternate Names:
    • BKCa; Slo; KCNMA1

    • KCa1.1
      View All» Usage Statement: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. View All» Key Applications: Western Blotting View All» Entrez Gene Summary: MaxiK channels are large conductance, voltage and calcium-sensitive potassium channels which are fundamental to the control of smooth muscle tone and neuronal excitability. MaxiK channels can be formed by 2 subunits: the pore-forming alpha subunit, which is the product of this gene, and the modulatory beta subunit. Intracellular calcium regulates the physical association between the alpha and beta subunits. Alternatively spliced transcript variants encoding different isoforms have been identified. View All» UniProt Summary: FUNCTION: SwissProt: Q12791 # Potassium channel activated by both membrane depolarization or increase in cytosolic Ca(2+) that mediates export of K(+). It is also activated by the concentration of cytosolic Mg(2+). Its activation dampens the excitatory events that elevate the cytosolic Ca(2+) concentration and/or depolarize the cell membrane. It therefore contributes to repolarization of the membrane potential. Plays a key role in controlling excitability in a number of systems, such as regulation of the contraction of smooth muscle, the tuning of hair cells in the cochlea, regulation of transmitter release, and innate immunity. In smooth muscles, its activation by high level of Ca(2+), caused by ryanodine receptors in the sarcoplasmic reticulum, regulates the membrane potential. In cochlea cells, its number and kinetic properties partly determine the characteristic frequency of each hair cell and thereby helps to establish a tonotopic map. Kinetics of KCNMA1 channels are determined by alternative splicing, phosphorylation status and its combination with modulating beta subunits. Highly sensitive to both iberiotoxin (IbTx) and charybdotoxin (CTX).SIZE: 1236 amino acids; 137560 Da SUBUNIT: Homotetramer; which constitutes the calcium-activated potassium channel. Interacts with beta subunits KCNMB1, KCNMB2, KCNMB3 and KCNMB4. Beta subunits are accessory, and modulate its activity.SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein.TISSUE SPECIFICITY: Widely expressed. Except in myocytes, it is almost ubiquitously expressed.DOMAIN: SwissProt: Q12791 The S0 segment is essential for the modulation by the accessory beta subunits KCNMB1, KCNMB2, KCNMB3 and KCNMB4. & The S4 segment, which is characterized by a series of positively charged amino acids at every third position, is part of the voltage-sensor. & The pore-forming domain (also referred as P region) is imbedded into the membrane, and forms the selectivity filter of the pore. It contains the signature sequence of potassium channels that displays selectivity to potassium. & The RCK N-terminal domain mediates the homotetramerization, thereby promoting the assembly of monomers into functional potassium channel. It includes binding sites for Ca(2+) and Mg(2+) (By similarity). & The calcium bowl constitutes one of the Ca(2+) sensors and probably acts as a Ca(2+)-binding site. There are however other Ca(2+) sensors regions required for activation of the channel.PTM: Phosphorylated (Probable). Phosphorylation by kinases such as PKA and/or PKG. In smooth muscles, phosphorylation affects its activity.DISEASE: SwissProt: Q12791 # Defects in KCNMA1 are the cause of generalized epilepsy and paroxysmal dyskinesia (GEPD) [MIM:609446]. Epilepsy is one of the most common and debilitating neurological disorders, affecting more than 40 million people worldwide. Paroxysmal dyskinesias are another heterogeneous group of neurological disorders characterized by sudden, unpredictable, disabling attacks of involuntary movement often requiring life-long treatment. The coexistence of epilepsy and paroxysmal dyskinesia in the same individual or family is an increasingly recognized phenomenon. The basic pathophysiology underlying the coexistence of these two disorders is caused by mutations in the KCNMA1 gene.SIMILARITY: Belongs to the potassium channel family. Calcium- activated subfamily. & Contains 1 RCK N-terminal domain.MISCELLANEOUS: The protein was initially thought to contain two functionally distinct parts: The core channel (from the N-terminus to the S9 segment) that mediates the channel activity, and the cytoplasmic tail (from the S9 segment to the C-terminus) that mediates the calcium sensing. The situation is however more complex, since the core channel also contains binding sites for Ca(2+) and Mg(2+). View All» Brand Family: Chemicon View All» Product Name: Anti-Potassium Channel MaxiK Antibody, calcium activated, aa1097-1196 | AB5228-200UL View All» Concentration: 60 μg/200 μL (after reconstitution) View All» Antibody Type: Polyclonal Antibody View All» Qty/Pk: 200 µL View All» Format: Affinity Purified View All» Host: Rabbit View All»
      © 金山科研平台是专业的授权总代理区域代理经销平台。
      © 如需询价，请加客服QQ：1749072012 、客服微信：jinshanbio，或发送邮件到1749072012@qq.com
      © 平台为生命科学研究相关领域提供一站式耗材试剂仪器解决方案和采购服务，数据资源基于CC协议。
      © 本文地址：https://www.16ao.com/thread-153903.htm
      © Merck Millipore代理AB5228-200UL Anti-Potassium Channel MaxiK Antibody, calcium activated, aa1097-1196，产品报价联系微信jinshanbio