货号：AB5382

品牌：Merck Millipore

规格：50UL

目录价：￥5053.00

市场价格：￥4295.05

会员价格：￥4042.40

金山科研平台，产品价格货期咨询微信：jinshanbio Description: Anti-Nitric Oxide Synthase II Antibody | AB5382 View All» Promotional Text: Special Shipping Offer on Antibodies100% Performance Guaranteed View All» Trade Name: Chemicon (Millipore) View All» Specificity: Inducible nitric oxide synthase (iNOS, NOS-II). No cross-reaction with rat nNOS and eNOS by Western blot and immunohistochemistry. View All» Molecular Weight: 131 kDa View All» Epitope: C-terminus View All» Immunogen: C-terminal 14 amino acid peptide from mouse macrophage nitric oxide synthase. View All» Background Information: Nitric Oxide Synthases are the only enzymes know to simultaneously require five bound cofactors, FAD, FMN, haem, tetrahydrobiopterin (BH4) and Ca++-Calmodulin (CAM). In mammals, there are three distinct genes for NOS, NOS I is neuronal, NOS II is cytokine inducible, and NOS III is endothelial. The enzymes exist as homodimers, each monomer consisting of two major domains: N­terminal oxygenase domain, which belongs to the class of haem-thiolate proteins, and C­terminal reductase domain, which is homologous to NADPH:P450 reductase. The interdomain linker between the oxygenase and reductase domains contains a CaM­binding sequence. Functionally NOS is an enzyme that catalyzes the stepwise conversion of the amino acid L-arginine to nitric oxide and L-citrulline. NOS activity stimulates cyclic GMP, inducing smooth muscle relaxation by increasing intracellular cGMP, which in turn inhibits calcium entry into the cell, and decreases intracellular calcium concentrations. This also stimulates a cGMP-dependent protein kinase that activates myosin light chain phosphatase, the enzyme that dephosphorylates myosin light chains, which leads to smooth muscle relaxation. View All» Species Reactivity:

• Mouse

• Rat
  View All» Species Reactivity Note: Mouse and rat. Reactivity to human iNOS is approximately 1%. View All» Application Notes: Immunohistochemistry: 1:5000 (ABC) dilution of a previous lot was used on tissue sections (rat liver).Immunocytochemistry: 1:50,000 dilution of a previous lot was used on cultured cells.Western blot: 1:5,000 (ECL). The antibody has been used successfully on rat liver. It is not recommended for use on rat brain extracts since unidentified bands may be present. Optimal working dilutions must be determined by end user. View All» Control: Macrophages, liver lysates View All» Quality Assurance: Evaluated by Western Blot on Mouse liver lysates.Western Blot Analysis: 1:1000 dilution of this antibody detected INOS on 10 µg of Mouse liver lysates. View All» Purification Method: Unpurified View All» Presentation: Rabbit polyclonal serum in buffer containing liquid 0.05% sodium azide. View All» Storage Conditions: Stable for 6 months at -20°C in undiluted aliquots from date of receipt. Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. View All» UniProt Number: P35228 View All» Entrez Gene Number: NM_153292.1 View All» Gene Symbol:
  • NOS2A

  • iNOS

  • HEP-NOS

  • INOS

  • NOS

  • NOS2

  • EC 1.14.13.39
    View All» Alternate Names:
    • NOS II

    • iNOS
      View All» Usage Statement: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. View All» Key Applications:
      • Immunocytochemistry

      • Immunohistochemistry

      • Western Blotting
        View All» Entrez Gene Summary: Nitric oxide is a reactive free radical which acts as a biologic mediator in several processes, including neurotransmission and antimicrobial and antitumoral activities. This gene encodes a nitric oxide synthase which is expressed in liver and is inducible by a combination of lipopolysaccharide and certain cytokines. Three related pseudogenes are located within the Smith-Magenis syndrome region on chromosome 17. View All» UniProt Summary: FUNCTION: SwissProt: P35228 # Produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body. In macrophages, NO mediates tumoricidal and bactericidal actions.COFACTOR: Heme group (By similarity). & Binds 1 FAD (By similarity). & Binds 1 FMN (By similarity). & Metrahydrobiopterin (BH4). May stabilize the dimeric form of the enzyme (By similarity).SIZE: 1153 amino acids; 131117 Da SUBUNIT: Homodimer. Binds SLC9A3R1.TISSUE SPECIFICITY: Expressed in the liver, retina, bone cells and airway epithelial cells of the lung. Not expressed in the platelets.SIMILARITY: SwissProt: P35228 ## Belongs to the NOS family. & Contains 1 flavodoxin-like domain. View All» Brand Family: Chemicon View All» Product Name: Anti-Nitric Oxide Synthase II Antibody | AB5382 View All» Concentration: Variable View All» Antibody Type: Polyclonal Antibody View All» Qty/Pk: 50 µL View All» Format: Serum View All» Host: Rabbit View All»
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