货号：AB5411

品牌：Merck Millipore

规格：100Ug

目录价：￥5820.00

市场价格：￥4947.00

会员价格：￥4656.00

金山科研平台，产品价格货期咨询微信：jinshanbio Description: Anti-Nitrotyrosine Antibody | AB5411 View All» Replaces: AB5386 View All» Promotional Text: Special Shipping Offer on Antibodies100% Performance Guaranteed View All» Trade Name: Chemicon (Millipore) View All» Specificity: Recognizes nitrated tyrosine residues. View All» Immunogen: Nitrated KLH. View All» Background Information: The cellular production of highly reactive nitrogen species derived from nitric oxide, such as peroxynitrite, nitrogen dioxide and nitryl chloride, leads to the nitration of tyrosine resides in tissue proteins. The extent of protein nitrotyrosine formation provides an index of the production of reactive nitrogen species and potential cell damage over a period of time. Nitrotyrosine can be measured by amino-acid analysis of protein hydrolysates and detected, estimated semi-quantitatively and located in cells and tissues by immunocytochemical techniques using antibodies directed against the nitrotyrosine hapten. View All» Species Reactivity: All View All» Species Reactivity Note: Human and mouse. Reactivity with other species has not been determined. View All» Application Notes: Western Blot: 1:100-1:1,000 dilution of a previous lot was used sucessfully in western blot when tested against nitrated BSA and nitrated ovalbumin.Immunohistochemistry: 1:50-1:100 on paraffin embedded, 10% neutral buffer formalin fixed mouse liver (animal treated with Zymosan) and normal human cerebellum tissues. The tissues must be pretreated with heat induced epitope retrieval (HIER). Incubation with the AB5411 was for 10 minutes and the reaction was developed with the Chemicon IHCSelect, HRP-DAB kit (Catalog number DAB150).Optimal working dilutions must be determined by end user. View All» Control: POSITIVE CONTROL: Tyrosine residues can be nitrated directly in situ on tissue sections using peroxynitrite (catalog # 20-107) to create a positive control. After the tissue section is deparaffinized, the slide is covered with a drop of PBS, pH 7.4 into which 10 microliters of peroxynitrite solution are mixed. Care should be taken that the solution maintains a pH of 7.4. View All» Quality Assurance: Immunohistochemistry(paraffin) Analysis:Nitrotyrosine (AB5411) staining of Mouse Brain (Zymosan), tissue pretreated with citrate buffer, pH 6.0. Polyclonal antibody was diluted to 1:500, using IHC-Select® Detection with HRP-DAB. Immunoreactivity is seen in here in hippocampus.Optimal Staining of Nitrotyrosine Monoclonal: Mouse Brain Treated With Zymosan. View All» Purification Method: Protein A purfied View All» Presentation: Purified rabbit polyclonal in buffer containing 0.1% sodium azide. View All» Storage Conditions: Stable for 1 year at 2-8ºC from date of receipt. View All» Usage Statement: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. View All» Key Applications:

• Immunohistochemistry (Paraffin)

• Western Blotting
  View All» Brand Family: Chemicon View All» Product Name: Anti-Nitrotyrosine Antibody | AB5411 View All» Concentration: 1.0 mg/mL View All» Antibody Type: Polyclonal Antibody View All» Qty/Pk: 100 μg View All» Format: Purified View All» Host: Rabbit View All»
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