货号：AB5477-200UL

品牌：Merck Millipore

规格：1SA

目录价：￥8226.00

市场价格：￥6992.10

会员价格：￥6580.80

金山科研平台，产品价格货期咨询微信：jinshanbio Description: Anti-P2X6 Receptor Antibody | AB5477-200UL View All» Promotional Text: Special Shipping Offer on Antibodies100% Performance Guaranteed View All» Trade Name: Chemicon (Millipore) View All» Specificity: Recognizes P2X6 Receptor. The epitope specific for the P2X6 receptor is not present in any other known proteins. SPECIES REACTIVITIES: The immunogen sequence is conserved in mouse (15/15) and human (14/17). Reactivity with other species has not yet been tested. View All» Immunogen: Highly purified peptide corresponding to residues 363-379 of rat P2X6 (Accession P51579). View All» Species Reactivity: Rat View All» Application Notes: Western blotting: 1:200 using ECL on rat brain membranes. Dilutions should be made using a carrier protein such as BSA (1-3%). Optimal working dilutions must be determined by the end user. SUGGESTED WESTERN BLOT PROTOCOL 1. Mix the samples (organ membranes: 50 μg/lane; transfected cells: 500,000 cells/lane) with sample-buffer X 2, and heat 10 min at 70°C. 2. 5-50 μL applied to Minigel lane (0.75-1.5 mm width) and run at standard conditions. (60 mA for 2 1.5 mm Minigel gels, 1.4 h). It is suggested that you run 5-15% acrylamide (37.5:1 acrylamide:bisacrysmide) minigel (1.5 mm width) at 30 mA/gel ~1-1.5 hours. 3. Transfer in semi-dry system under standard conditions (3 h 100 mA for two minigel gels) 4. Stain the transferred bands with Chemicon BLOT-FastStain (Catalog Number 2076). 5. Destain with deionized water. 6. Block with 5% non-fat milk (Marvel or Carnation) in PBS, and 0.025 % sodium azide, overnight at 2-8°C. The non-fat milk should be dissolved freshly, centrifuged 10,000 rpm for 10 min, and filtered through glass filter (Gelman Acrodisc). 7. Incubation with first antibody 2 h at room temperature or overnight at 4°C in blocking solution. The antibody preparation should be centrifuged before use (10,000 g 5 min.). Optimal working dilutions and incubation time will need to be determined by the end user. 8. Wash 4 x 10 min. with PBS-0.1% tween 20. From this stage, azide should be omitted. 9. Incubation with the secondary antibody (HRP-conjugated goat anti-rabbit antibody, for example Chemicon Catalog Number AP132P, diluted appropriately) 1 h at room temperature. 10. Wash 4 x 10 min. with PBS-0.1% tween 20. 11. Perform ECL with commercial kits (ChemiLUCENT, Chemicon Catalog Number 2600). View All» Control: Included free of charge with the antibody is 40 μg of control antigen (lyophilized powder). For negative control, preincubate 1 μg of purified peptide with 1 μg of antibody for one hour at room temperature. Optimal concentrations must be determined by the end user. View All» Presentation: Affinity purified immunoglobulin. Lyophilized from PBS, pH 7.4, containing 1% BSA and 0.05% sodium azide. Reconstitute with 200μL of sterile distilled water. Centrifuge antibody preparation before use (10,000 x g for 5 min). View All» Storage Conditions: Maintain lyophilized material at -20°C for up to 6 months after date of receipt. After reconstitution maintain at -20°C in undiluted aliquots for up to 6 months. Avoid repeated freeze/thaw cycles. View All» UniProt Number: O15547 View All» Entrez Gene Number: NM_005446.3 View All» Gene Symbol:

• P2RXL1

• P2RX6

• P2XM

• MGC129625

• P2X6
  View All» Usage Statement: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. View All» Key Applications: Western Blotting View All» Entrez Gene Summary: The protein encoded by this gene belongs to the family of P2X receptors, which are ATP-gated ion channels and mediate rapid and selective permeability to cations. This gene is predominantly expressed in skeletal muscle, and regulated by p53. The encoded protein is associated with VE-cadherin at the adherens junctions of human umbilical vein endothelial cells. View All» UniProt Summary: FUNCTION: SwissProt: O15547 # Receptor for ATP that acts as a ligand gated ion channel (By similarity).SIZE: 431 amino acids; 47913 Da SUBUNIT: Homo- or heteropolymers (By similarity).SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein.TISSUE SPECIFICITY: Expressed predominantly in skeletal muscle.SIMILARITY: SwissProt: O15547 ## Belongs to the P2X receptor family. View All» Brand Family: Chemicon View All» Product Name: Anti-P2X6 Receptor Antibody | AB5477-200UL View All» Concentration: 0.65 mg/mL (after reconstitution) View All» Antibody Type: Polyclonal Antibody View All» Qty/Pk: 200 µL View All» Format: Affinity Purified View All» Host: Rabbit View All»
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