货号：AB5481-50UL

品牌：Merck Millipore

规格：

目录价：￥4282.00

市场价格：￥3639.70

会员价格：￥3425.60

金山科研平台，产品价格货期咨询微信：jinshanbio Description: Anti-Potassium Channel EAG-2 View All» Promotional Text: Special Shipping Offer on Antibodies100% Performance Guaranteed View All» Trade Name: Chemicon (Millipore) View All» Specificity: Recognizes EAG-2 (Ether-a-go-go potassium channel 2, KCNH5). The epitope specific for EAG-2 is not present in any other known proteins. SPECIES REACTIVITIES: The immunogen sequence is conserved in human (18/19). Reactivity with other species has not yet been tested. View All» Immunogen: Highly purified peptide corresponding to residues 842-860 of rat EAG-2 (Accession Q9EPI9). View All» Species Reactivity: Rat View All» Application Notes: Western blotting: 1:200 using ECL on rat brain membranes. See suggested protocol. Dilutions should be made using a carrier protein such as BSA (1-3%). Optimal working dilutions must be determined by the end user.SUGGESTED WESTERN BLOT PROTOCOL1. Mix the samples (organ membranes: 50 μg/lane; transfected cells: 500,000 cells/lane) with sample-buffer X 2, and heat 10 min at 70°C.2. 5-50 μL applied to Minigel lane (0.75-1.5 mm width) and run at standard conditions. (60 mA for 2 1.5 mm Minigel gels, 1.4 h). It is suggested that you run 5-15% acrylamide (37.5:1 acrylamide:bisacrysmide) minigel (1.5 mm width) at 30 mA/gel ~1-1.5 hours.3. Transfer in semi-dry system under standard conditions (3 h 100 mA for two minigel gels)4. Stain the transferred bands with Chemicon BLOT-FastStain (Catalog Number 2076).5. Destain with deionized water.6. Block with 5% non-fat milk (Marvel or Carnation) in PBS, and 0.025 % sodium azide, overnight at 2-8°C. The non-fat milk should be dissolved freshly, centrifuged 10,000 rpm for 10 min, and filtered through glass filter (Gelman Acrodisc).7. Incubation with first antibody 2 h at room temperature or overnight at 4°C in blocking solution. The antibody preparation should be centrifuged before use (10,000 g 5 min.). Optimal working dilutions and incubation time will need to be determined by the end user.8. Wash 4 x 10 min. with PBS-0.1% tween 20. From this stage, azide should be omitted.9. Incubation with the secondary antibody (HRP-conjugated goat anti-rabbit antibody, for example Chemicon Catalog Number AP132P, diluted appropriately) 1 h at room temperature.10. Wash 4 x 10 min. with PBS-0.1% tween 20.11. Perform ECL with commercial kits (Chemilucent, Chemicon Catalog Number 2600). View All» Control: Included free of charge with the antibody is 40 μg of control antigen (lyophilized powder). The stock solution of the antigen can be made up using 100 μL of sterile distilled water. For negative control, preincubate 1 μg of purified peptide with 1 μg of antibody for one hour at room temperature. Optimal concentrations must be determined by the end user. View All» Presentation: Affinity purified immunoglobulin. Lyophilized from PBS, pH 7.4, containing 1% BSA, 5% sucrose and 0.025% sodium azide. Reconstitute with 50 μL of sterile distilled water. Centrifuge antibody preparation before use (10,000 x g for 5 min). View All» Storage Conditions: Maintain lyophilized material at -20°C for up to 6 months after date of receipt. After reconstitution maintain at -20°C in undiluted aliquots for up to 6 months. Avoid repeated freeze/thaw cycles. View All» UniProt Number: Q8NCM2 View All» Entrez Gene Number:

• NM_139318.3

• NM_172375.1

• NM_172376.1
  View All» Gene Symbol:
  • KCNH5

  • eag2

  • hEAG2

  • EAG2

  • Eag2

  • H-EAG2

  • Kv10.2
    View All» Alternate Names: Ether-a-go-go Potassium Channel 2; KCNH5 View All» Usage Statement: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. View All» Key Applications: Western Blotting View All» Entrez Gene Summary: Voltage-gated potassium (Kv) channels represent the most complex class of voltage-gated ion channels from both functional and structural standpoints. Their diverse functions include regulating neurotransmitter release, heart rate, insulin secretion, neuronal excitability, epithelial electrolyte transport, smooth muscle contraction, and cell volume. This gene encodes a member of the potassium channel, voltage-gated, subfamily H. This member is a pore-forming (alpha) subunit of a voltage-gated non-inactivating delayed rectifier potassium channel. This gene is not expressed in differentiating myoblasts. Alternative splicing results in three transcript variants encoding distinct isoforms. View All» UniProt Summary: FUNCTION: SwissProt: Q8NCM2 # Pore-forming (alpha) subunit of voltage-gated potassium channel. Elicits a non-inactivating outward rectifying current. Channel properties may be modulated by cAMP and subunit assembly.SIZE: 988 amino acids; 111907 Da SUBUNIT: The potassium channel is probably composed of a homo- or heterotetrameric complex of pore-forming alpha subunits that can associate with modulating beta subunits. Heteromultimer with KCNH1/EAG (Probable).SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein.TISSUE SPECIFICITY: Detected in brain, skeletal muscle, heart, placenta, lung and liver, and at low levels in kidney.DOMAIN:SwissProt: Q8NCM2 The segment S4 is probably the voltage-sensor and is characterized by a series of positively charged amino acids at every third position.SIMILARITY: Belongs to the potassium channel family. H (Eag) subfamily. & Contains 1 cyclic nucleotide-binding domain. & Contains 1 PAC (PAS-associated C-terminal) domain. & Contains 1 PAS (PER-ARNT-SIM) domain. View All» Brand Family: Chemicon View All» Product Name: Anti-Potassium Channel EAG-2 View All» Concentration: 0.8 mg/mL (after reconstitution) View All» Antibody Type: Polyclonal Antibody View All» Qty/Pk: 50 µL View All» Format: Affinity Purified View All» Host: Rabbit View All»
    © 金山科研平台是专业的授权总代理区域代理经销平台。
    © 如需询价，请加客服QQ：1749072012 、客服微信：jinshanbio，或发送邮件到1749072012@qq.com
    © 平台为生命科学研究相关领域提供一站式耗材试剂仪器解决方案和采购服务，数据资源基于CC协议。
    © 本文地址：https://www.16ao.com/thread-158049.htm
    © Merck Millipore代理AB5481-50UL EAG-2 POTASSIUM CHAN, RBX-50UL，产品报价联系微信jinshanbio