货号：AB5490

品牌：Merck Millipore

规格：100Ug

目录价：￥5096.00

市场价格：￥4331.60

会员价格：￥4076.80

金山科研平台，产品价格货期咨询微信：jinshanbio Description: Anti-Atrial Natriuretic Peptide Antibody | AB5490 View All» Promotional Text: Special Shipping Offer on Antibodies100% Performance Guaranteed View All» Trade Name: Chemicon (Millipore) View All» Specificity: Recognizes Atrial Natriuretic Peptide (ANP). View All» Immunogen: Rat ANP conjugated to BSA. View All» Species Reactivity:

• Mouse

• Rat
  View All» Application Notes: Immunohistochemistry: 2-10 μg/mL. See suggested protocol below. Immunoprecipitation RIA Optimal working dilutions must be determined by the end user. IMMUNOHISTOCHEMISTRY PROTOCOL. Anesthetize animals and perfuse them transcardially as follows: a) Flush with cold (4°C) oxygenated Calcium free Tyrodes b) Perfuse with 4% formaldehyde in 0.16M phosphate buffer at pH 6.9 c) Perfuse with 10% sucrose solution in 0.1M phosphate buffer (pH 7.2)Sections Cut 5-30 mm sections using a cryostat and mount them on subbed histological slides.ImmunofluorescenceDilute ANP antibody with 0.1M phosphate buffered saline (PBS, pH 7.4) containing 1% BSA and 0.1% Triton X-100. Incubate sections for 24-48 hours at 2-8°C. Wash in PBS (three times for 10 minutes). Incubate for one hour or two hours at room temperature with properly diluted donkey anti-rabbit secondary antibody conjugated to a fluorescent probe such as FITC (Catalog Number AP182F), Rhodamine (Catalog Number AB182R) or Cy3 (Catalog Number AP182C). Wash in PBS (three times for 10 minutes) and mount with a PBS/glycerol solution containing 0.1% phenylenediamine to reduce fading. If not stained with FITC, sections can be dehydrated in grading alcohols (50%, 75%, 80%, 96% and 100%), cleared with xylene and mounted with DPX (Fluka, Ronkonkoma, NY). Staining can be visualized by using both conventional and confocal microscopy.Indirect Avidin-Biotin PeroxidaseIncubate sections with 0.3% H2O2 in PBS for 15 minutes at room temperature to block endogenous peroxidase. Rinse sections with PBS (three times for 10 minutes). Dilute ANP antibody with 0.1M phosphate buffered saline (PBS, pH 7.4) containing 1% BSA and 0.1% Triton X-100. Incubate sections overnight at 2-8°C and then wash in PBS (three times for 10 minutes). Incubate sections with properly diluted biotinilated goat anti-rabbit secondary antibody (Catalog Number AP132B) diluted in PBS (do not add sodium azide!) for one hour at room temperature. Rinse sections three times for 15 minutes and incubate sections with horseradish peroxidase-streptavidin complex (Catalog Number SA108) properly diluted in PBS/0.1% Triton X-100 for 40 minutes at room temperature. Wash sections PBS and incubate them in substrate solutions (e.g. DAB, AEC, etc.) to achieve necessary intensity of ANP staining. View All» Control: Heart tissue View All» Purification Method: Immunoaffinity purified View All» Presentation: Affinity Purified immunoglobulin. Liquid containing 0.1% sodium azide. View All» Storage Conditions: Maintain for 1 year at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. View All» UniProt Number: P01160 View All» Entrez Gene Number: NM_006172.2 View All» Gene Symbol:
  • NPPA

  • CDD-ANF

  • ANP

  • PND

  • ANF

  • pronatriodilatin

  • OTTHUMP00000044485

  • Prepronatriodilatin
    View All» Alternate Names: ANP View All» Usage Statement: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. View All» Key Applications:
    • Immunohistochemistry

    • Immunoprecipitation

    • Radioimmunoassay
      View All» UniProt Summary: FUNCTION: SwissProt: P01160 # A potent vasoactive substance which is thought to play a key role in cardiovascular homeostasis. Has a cGMP-stimulating activity.SIZE: 153 amino acids; 16708 Da SUBUNIT: Interacts with NPR3.SUBCELLULAR LOCATION: Secreted.SIMILARITY: SwissProt: P01160 ## Belongs to the natriuretic peptide family. View All» Brand Family: Chemicon View All» Product Name: Anti-Atrial Natriuretic Peptide Antibody | AB5490 View All» Concentration: 1 mg/mL View All» Antibody Type: Polyclonal Antibody View All» Qty/Pk: 100 μg View All» Format: Affinity Purified View All» Host: Rabbit View All»
      © 金山科研平台是专业的授权总代理区域代理经销平台。
      © 如需询价，请加客服QQ：1749072012 、客服微信：jinshanbio，或发送邮件到1749072012@qq.com
      © 平台为生命科学研究相关领域提供一站式耗材试剂仪器解决方案和采购服务，数据资源基于CC协议。
      © 本文地址：https://www.16ao.com/thread-158055.htm
      © Merck Millipore代理AB5490 Anti-Atrial Natriuretic Peptide Antibody;store at -20℃，产品报价联系微信jinshanbio