货号：AB5708

品牌：Merck Millipore

规格：100Ug

目录价：￥5666.00

市场价格：￥4816.10

会员价格：￥4532.80

金山科研平台，产品价格货期咨询微信：jinshanbio Description: Anti-HES-5 Antibody | AB5708 View All» Promotional Text: Special Shipping Offer on Antibodies100% Performance Guaranteed View All» Trade Name: Chemicon (Millipore) View All» Specificity: Recognizes HES-5 (Hairy family), a Helix-loop-helix class of transcription factor. View All» Immunogen: Synthetic peptide, amino acids 83-95 of mouse HES-5. View All» Background Information: HES-5 is a mammalian basic helix-loop-helix factor that has a distant sequence homology to the product of the Drosophila pair-rule gene hairy. HES-5 mRNA is present exclusively in the developing nervous system, but its level decreases as neural differentiation proceeds. View All» Species Reactivity:

• Human

• Mouse
  View All» Application Notes: Western blot: 1:200-1:1,000 using ECL. Detects a 28kDa band in embryonal RN46a rat cell line {http://www.jneurosci.org/cgi/reprint/23/25/8788.pdf} Immunohistochemistry: 1:200-1:1,000. Optimal working dilutions must be determined by the end user. View All» Presentation: Affinity purified immunoglobulin. Precipitated antibody in a solution of 50% saturated ammonium sulfate and PBS containing no preservatives. View All» Storage Conditions: Maintain unopened vial at -70°C for up to 6 months. Avoid repeated freeze/thaw cycles. PREPARATION AND USE: To reconstitute the antibody, centrifuge the antibody vial at moderate speed (5,000 rpm) for 5 minutes to pellet the precipitated antibody product. Carefully remove the ammonium sulfate/PBS buffer solution and discard. It is not necessary to remove all of the ammonium sulfate/PBS solution: 10 μL of residual ammonium sulfate solution will not affect the resuspension of the antibody. Do not let the protein pellet dry, as severe loss of antibody reactivity can occur. Resuspend the antibody pellet in a suitable biological buffer such as PBS or TBS (pH 7.3-7.5) to a final concentration of 1.0 mg/mL. For example, to achieve a 1.0 mg/mL concentration with 50 μg of precipitated antibody, the amount of buffer needed would be 50 μL. Carefully add the liquid buffer to the pellet. DO NOT VORTEX. Mix by gentle stirring with a wide pipet tip or gentle finger-tapping. Let the precipitated antibody rehydrate for 1 hour at 4-25°C prior to use. Small particles of precipitated antibody that fail to resuspend are normal. Vials are overfilled to compensate for any losses. The rehydrated antibody solutions can be stored undiluted at 2-8°C for 2 months without any significant loss of activity. Note, the solution is not sterile, thus care should be taken if product is stored at 2-8°C. For storage at -20°C, the addition of an equal volume of glycerol can be used, however, it is recommended that ACS grade or higher glycerol be used, as significant loss of activity can occur if the glycerol used is not of high quality. For long-term storage at -70°C, it is recommended that the rehydrated antibody solution be further diluted 1:1 with a 2% BSA (fraction V, highest-grade available) solution made with the rehydration buffer. The resulting 1% BSA/antibody solution can be aliquoted and stored frozen at -70°C for up to 6 months. Avoid repeated freeze/thaw cycles. View All» UniProt Number: Q5TA89 View All» Entrez Gene Number: NM_001010926.2 View All» Gene Symbol: HES5 View All» Alternate Names: Hairy 5 View All» Usage Statement: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. View All» Key Applications:
  • Western Blotting

  • Immunohistochemistry
    View All» UniProt Summary: FUNCTION: SwissProt: Q5TA89 # Transcriptional repressor of genes that require a bHLH protein for their transcription (By similarity).SIZE: 166 amino acids; 18226 Da SUBUNIT: Transcription repression requires formation of a complex with a corepressor protein (Groucho) (By similarity).SUBCELLULAR LOCATION: Nucleus (By similarity).TISSUE SPECIFICITY: Expressed in fetal heart and brain tumors.DOMAIN: SwissProt: Q5TA89 Has a particular type of basic domain (presence of a helix-interrupting proline) that binds to the N-box (CACNAG), rather than the canonical E-box (CANNTG) (By similarity). & The C-terminal WRPW motif is a transcriptional repression domain necessary for the interaction with Groucho, a transcriptional corepressor recruited to specific target DNA by Hairy-related proteins (By similarity).SIMILARITY: Contains 1 basic helix-loop-helix (bHLH) domain. & Contains 1 Orange domain. View All» Brand Family: Chemicon View All» Product Name: Anti-HES-5 Antibody | AB5708 View All» Antibody Type: Polyclonal Antibody View All» Qty/Pk: 100 µg View All» Target / Localization: Nuclear View All» Format: Affinity Purified View All» Host: Rabbit View All»
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