货号：AB5894

品牌：Merck Millipore

规格：50UL

目录价：￥4746.00

市场价格：￥4034.10

会员价格：￥3796.80

金山科研平台，产品价格货期咨询微信：jinshanbio Description: Anti-P2X2 Receptor Antibody | AB5894 View All» Promotional Text: Special Shipping Offer on Antibodies100% Performance Guaranteed View All» Trade Name: Chemicon (Millipore) View All» Specificity: P2X2 Receptor. View All» Immunogen: A 13 amino acid peptide corresponding to amino acids 460-472 from the carboxy-terminus of the rat P2X2 Receptor protein. Control peptide: Catalog Number AG354 View All» Species Reactivity:

• Human

• Rat
  View All» Application Notes: Immunoblotting: 1:500 Immunohistochemistry: 1:500. Immunocytochemistry: 1:500. Optimal working dilutions must be determined by end user. APPLICATION NOTES FOR AB5894 IMMUNOHISTOCHEMISTRY Male Sprague-Dawley rats (b.wt. 100-150g) were anesthetized with sodium pentobarbital and perfused via the ascending aorta with: 1) 50 mL of Ca2+-free Tyrode+s solution followed by 2) a formalin-picric acid fixative (4% paraformaldehyde with 0.4% picric acid in 0.16 M phosphate buffer, pH 6.9) and 3) 10% sucrose in PBS as a cryo-protectant. Tissues were rapidly dissected out and stored overnight in 0.1 M phosphate buffer (pH 7.4) containing 10% sucrose. Slide-mounted tissue sections were incubated with blocking buffer for 1 hour at room temperature. Primary antibody was diluted in blocking buffer to the appropriate working dilution. Blocking buffer was removed and the slides were then incubated at 2-8°C for 18-24 hours with AB5894 (1:500). After rinsing in PBS 3 times sections were incubated for 60 minutes at room temperature with Cy3-conjugated secondary antibodies. After mounting in a mixture of PBS and glycerol (1:3) containing 0.1% p-phenylenediamine, sections were examined with a Nikon Microphot-SA epifluorescence microscope. IMMUNOCYTOCHEMISTRYP 2X2 transfected cells were processed for indirect immunofluorescence. Media was removed and cells were gently washed 3 times with serum-free media. Following fixation procedure, cells were processed for indirect immunofluorescence as above WESTERN BLOTTING Cell membrane extracts were examined by electrophoresis (8% acrylamide) with SDS under reducing conditions and transferred to a nylon membrane. Membranes were blocked for 1 hour at 2-8°C with 0.1% Tween 20 and 2.5% milk powder (w/v) in PBS. Membranes were incubated with AB5894 diluted 1:500 with same buffer overnight at 2-8°C. Membranes were rinsed and incubated with HRP conjugated secondary antibody for 1 hour at room temperature. Following rinsing the membranes were processed using enhanced chemiluminescence. View All» Presentation: Serum. Liquid. Contains 0.05% sodium azide. View All» Storage Conditions: Maintain at -20°C in undiluted aliquots for up to 6 months. Avoid repeated freeze/thaw cycles. View All» UniProt Number: Q9UBL9 View All» Entrez Gene Number:
  • NM_012226.3

  • NM_016318.2

  • NM_170682.2

  • NM_170683.2

  • NM_174872.1

  • NM_174873.1
    View All» Gene Symbol:
    • P2RX2

    • MGC129601

    • P2X2
      View All» Usage Statement: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. View All» Key Applications:
      • Immunocytochemistry

      • Immunohistochemistry

      • Western Blotting
        View All» Entrez Gene Summary: The product of this gene belongs to the family of purinoceptors for ATP. This receptor functions as a ligand-gated ion channel. Binding to ATP mediates synaptic transmission between neurons and from neurons to smooth muscle. Six transcript variants encoding six distinct isoforms have been identified for this gene. View All» UniProt Summary: FUNCTION: SwissProt: Q9UBL9 # Binding of this ligand gated ion channel to ATP mediates synaptic transmission between neurons and from neurons to smooth muscle.SIZE: 471 amino acids; 51754 Da SUBUNIT: Homo- or heteropolymers.SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein.SIMILARITY: SwissProt: Q9UBL9 ## Belongs to the P2X receptor family. View All» Brand Family: Chemicon View All» Product Name: Anti-P2X2 Receptor Antibody | AB5894 View All» Antibody Type: Polyclonal Antibody View All» Qty/Pk: 50 µL View All» Format: Serum View All» Host: Guinea Pig View All»
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