货号：AB5895

品牌：Merck Millipore

规格：50UL

目录价：￥5974.00

市场价格：￥5077.90

会员价格：￥4779.20

金山科研平台，产品价格货期咨询微信：jinshanbio Description: Anti-P2X3 Receptor Antibody, pain | AB5895 View All» Replaces: AB5896 View All» Promotional Text: Special Shipping Offer on Antibodies100% Performance Guaranteed View All» Trade Name: Chemicon (Millipore) View All» Specificity: P2X3 SPECIES REACTIVITIES: Rat, mouse and primate. Other species have not been tested. View All» Immunogen: Synthetic peptide corresponding to the C-terminus of rat P2X3. View All» Species Reactivity:

• Rat

• Primate

• Human
  View All» Application Notes: Immunoblotting: 1:500-1:1,000 Immunohistochemistry: 1:500-1:1,000 Immunocytochemistry: 1:500-1:1,000 Optimal working dilutions must be determined by the end user. APPLICATION NOTES FOR AB5895 IMMUNOHISTOCHEMISTRY Male Sprague-Dawley rats (b.wt. 100-150g) were anesthetized with sodium pentobarbital and perfused via the ascending aorta with: 1) 50 mL of Ca2+-free Tyrode+s solution followed by 2) fixative, and 3) 10% sucrose in PBS as a cryo-protectant. Tissues were rapidly dissected out and stored overnight in 0.1 M phosphate buffer (pH 7.4) containing 10% sucrose. Slide-mounted tissue sections were incubated with blocking buffer for 1 hour at room temperature. Primary antibody was diluted in blocking buffer to the appropriate working dilution. Blocking buffer was removed and the slides were then incubated at 2-8°C for 18-24 hours with AB5895. After rinsing in PBS 3 times sections were incubated for 60 minutes at room temperature with Cy3-conjugated secondary antibodies. After mounting in a mixture of PBS and glycerol (1:3) containing 0.1% p-phenylenediamine, sections were examined with a Nikon Microphot-SA epifluorescence microscope. IMMUNOCYTOCHEMISTRY P2X3 transfected cells were processed for indirect immunofluorescence. Media was removed and cells were gently washed 3 times with serum-free media. Following fixation procedure, cells were processed for indirect immunofluorescence as described above. View All» Presentation: Rabbit serum. Liquid. Contains 0.05% sodium azide. View All» Storage Conditions: Maintain at -20°C in undiluted for up to 6 months after date of receipt. Avoid repeated freeze/thaw cycles. Do not store in a self-defrosting freezer. View All» UniProt Number: P56373 View All» Entrez Gene Number: NM_002559.2 View All» Gene Symbol:
  • P2RX3

  • MGC129956

  • P2X3
    View All» Usage Statement: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. View All» Key Applications:
    • Immunocytochemistry

    • Immunohistochemistry

    • Western Blotting
      View All» Entrez Gene Summary: The product of this gene belongs to the family of purinoceptors for ATP. This receptor functions as a ligand-gated ion channel and may transduce ATP-evoked nociceptor activation. Mouse studies suggest that this receptor is important for peripheral pain responses, and also participates in pathways controlling urinary bladder volume reflexes. It is possible that the development of selective antagonists for this receptor may have a therapeutic potential in pain relief and in the treatment of disorders of urine storage. View All» UniProt Summary: FUNCTION: SwissProt: P56373 # Receptor for ATP that acts as a ligand gated ion channel.SIZE: 397 amino acids; 44230 Da SUBUNIT: Homo- or heteropolymers (By similarity).SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein.SIMILARITY: SwissProt: P56373 ## Belongs to the P2X receptor family. View All» Brand Family: Chemicon View All» Product Name: Anti-P2X3 Receptor Antibody, pain | AB5895 View All» Antibody Type: Polyclonal Antibody View All» Qty/Pk: 50 µL View All» Format: Serum View All» Host: Rabbit View All»
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