货号：AB5945

品牌：Merck Millipore

规格：100Ug

目录价：￥5974.00

市场价格：￥5077.90

会员价格：￥4779.20

金山科研平台，产品价格货期咨询微信：jinshanbio Description: Anti-Brn-3a Antibody | AB5945 View All» Promotional Text: Special Shipping Offer on Antibodies100% Performance Guaranteed View All» Trade Name: Chemicon (Millipore) View All» Specificity: Recognizes Brn3a. The molecular weight of the protein is 42.9kD. View All» Molecular Weight: 42.9 kDa View All» Immunogen: A synthetic peptide corresponding to human Brn3a (amino acids 141-157) conjugated to BSA. View All» Species Reactivity:

• Human

• Mouse
  View All» Application Notes: Western blot: 1:200-1:1,000 using ECL. Immunohistochemistry: 1:200-1:1,000. Immunocytochemistry: 1:200-1:1,000. Optimal working dilutions must be determined by the end user. View All» Control: Widely expressed View All» Purification Method: Immunoaffinity purified View All» Presentation: Affinity Purified immunoglobulin. Precipitated antibody in a solution of 50% saturated ammonium sulfate and PBS containing no preservatives. View All» Storage Conditions: Maintain unopened vial at -70°C for up to 6 months. Avoid repeated freeze/thaw cycles. PREPARATION AND USE: To reconstitute the antibody, centrifuge the antibody vial at moderate speed (5,000 rpm) for 5 minutes to pellet the precipitated antibody product. Carefully remove the ammonium sulfate/PBS buffer solution and discard. It is not necessary to remove all of the ammonium sulfate/PBS solution: 10 μL of residual ammonium sulfate solution will not affect the resuspension of the antibody. Do not let the protein pellet dry, as severe loss of antibody reactivity can occur. Resuspend the antibody pellet in a suitable biological buffer such as PBS or TBS (pH 7.3-7.5) to a final concentration of 1.0 mg/mL. For example, to achieve a 1.0 mg/mL concentration with 50 μg of precipitated antibody, the amount of buffer needed would be 50 μL. Carefully add the liquid buffer to the pellet. DO NOT VORTEX. Mix by gentle stirring with a wide pipet tip or gentle finger-tapping. Let the precipitated antibody rehydrate for 1 hour at 4-25°C prior to use. Small particles of precipitated antibody that fail to resuspend are normal. Vials are overfilled to compensate for any losses. The rehydrated antibody solutions can be stored undiluted at 2-8°C for 2 months without any significant loss of activity. Note, the solution is not sterile, thus care should be taken if product is stored at 2-8°C. For storage at -20°C, the addition of an equal volume of glycerol can be used, however, it is recommended that ACS grade or higher glycerol be used, as significant loss of activity can occur if the glycerol used is not of high quality. For long-term storage at -70°C, it is recommended that the rehydrated antibody solution be further diluted 1:1 with a 2% BSA (fraction V, highest-grade available) solution made with the rehydration buffer. The resulting 1% BSA/antibody solution can be aliquoted and stored frozen at -70°C for up to 6 months. Avoid repeated freeze/thaw cycles. View All» UniProt Number: Q01851 View All» Entrez Gene Number: NM_006237.3 View All» Gene Symbol:
  • POU4F1

  • FLJ13449

  • Brn-3A

  • RDC-1

  • Oct-T1

  • BRN3A

  • RDC1
    View All» Usage Statement: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. View All» Key Applications:
    • Immunocytochemistry

    • Immunohistochemistry

    • Western Blotting
      View All» Entrez Gene Summary: BRN3A (POU4F1) is a class IV POU domain-containing transcription factor highly expressed in the developing sensory nervous system and in cells of the B- and T-lymphocytic lineages (Gerrero et al., 1993 [PubMed 8248179]).[supplied by OMIM] View All» UniProt Summary: FUNCTION: SwissProt: Q01851 # Probable transcription factor which may play a role in the regulation of specific gene expression within a subset of neuronal lineages. May play a role in determining or maintaining the identities of a small subset of visual system neurons.SIZE: 423 amino acids; 42940 Da SUBCELLULAR LOCATION: Nucleus.TISSUE SPECIFICITY: Brain. Seems to be specific to the retina. Present in the developing brain, spinal cord and eye.DEVELOPMENTAL STAGE: Expression peaks early in embryogenesis (day 13.5) and is undetectable 14 days after birth.SIMILARITY: SwissProt: Q01851 ## Belongs to the POU transcription factor family. Class- 4 subfamily. & Contains 1 homeobox DNA-binding domain. & Contains 1 POU-specific domain. View All» Brand Family: Chemicon View All» Product Name: Anti-Brn-3a Antibody | AB5945 View All» Antibody Type: Polyclonal Antibody View All» Qty/Pk: 100 μg View All» Format: Affinity Purified View All» Host: Rabbit View All»
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