货号:AB8901
品牌:Merck Millipore
规格:
目录价:询价
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Description:
Anti-Neutral Amino Acid Transporter Antibody | AB8901
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Promotional Text:
Special Shipping Offer on Antibodies100% Performance Guaranteed
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Trade Name:
Chemicon (Millipore)
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Specificity:
AB8901 is a polyclonal antibody raised against a 12 amino acid sequence corresponding to the NH2 terminus of the neutral amino acid transporter ASCT1, which is also involved in glutamine transport (Wasa et al. 1996). The antibody has also been described in the literature as the "MYS" antibody. The antibody detects ASCT1 by immunohistochemistry in sections of formalin-fixed and paraffin-embedded tissues of human origin. In general, there is weak, and rarely strong, positive ASCT1 immunostaining in many normal cell types. By contrast, strong ASCT1 staining is detectable in many human cancers including those of the colon, lung, esophagus, and breast. Whereas ASCT1 overexpression is associated with better survival in non-small cell stage I lung carcinomas, it is associated with worse survival in carcinomas of the esophagus.
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Species Reactivity:
Human
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Application Notes:
Immunoblotting: 5 μg/mL. Theoretical size is 56 kDa; however, should run ~ 62 kDa. Immunocytochemistry (HepG2 cells) Immunohistochemistry on formalin-fixed, paraffin-embedded tissues: 5 μg/mL. After deparaffinization and rehydration, tissue sections should be treated with an antigen retrieval method, such as steaming in 10 mM citrate buffer pH 6.0 for 30 minutes, then blocking non-specific binding with 2% normal goat serum in 1% BSA in PBS. Sections are then incubated at room temperature for 2 hours with 5 μg/mL dilution of AB8901 in 0.1% BSA in PBS, pH 7.4. the bound antibody can be detected using any of the commercially available detection kits. Final dilution will depend on the sensitivity of the detection kit/system used. Optimal working dilutions must be determined by the end user.
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Presentation:
Purified immunoglobulin - Ig fraction. Liquid at 1 mg/mL in phosphate buffered saline (PBS), pH 7.4, containing .025% sodium azide and .075% BSA.
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Storage Conditions:
Store at -20°C. Avoid repeated freeze-thaw cycles.
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UniProt Number:
P43007
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Entrez Gene Number:
NM_003038.2
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Gene Symbol:
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SLC1A4
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SATT
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ASCT1
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Alternate Names:
ASCT1
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Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Key Applications:
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Immunocytochemistry
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Immunohistochemistry (Paraffin)
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Western Blotting
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UniProt Summary:
FUNCTION: SwissProt: P43007 # Transporter for alanine, serine, cysteine, and threonine. Exhibits sodium dependence.SIZE: 532 amino acids; 55723 Da SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein. Melanosome. Note=Identified by mass spectrometry in melanosome fractions from stage I to stage IV.TISSUE SPECIFICITY: Expressed mostly in brain, muscle, and pancreas but detected in all tissues examined.SIMILARITY: SwissProt: P43007 ## Belongs to the sodium:dicarboxylate (SDF) symporter (TC 2.A.23) family.
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Brand Family:
Chemicon
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Product Name:
Anti-Neutral Amino Acid Transporter Antibody | AB8901
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Antibody Type:
Polyclonal Antibody
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Qty/Pk:
50 µg
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Format:
Purified
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Host:
Rabbit
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