货号：AB9018-50UL

品牌：Merck Millipore

规格：1EA

目录价：￥5681.64

市场价格：￥4829.39

会员价格：￥4545.31

金山科研平台，产品价格货期咨询微信：jinshanbio Description: Anti-Muscarinic Acetylcholine Receptor m3 Antibody | AB9018-50UL View All» Promotional Text: Special Shipping Offer on Antibodies100% Performance Guaranteed View All» Trade Name: Chemicon (Millipore) View All» Specificity: Muscarinic acetylcholine receptor m3. View All» Immunogen: Synthetic peptide from the 3rd extracellular loop of rat m3 (Accession P08483). The immunogen sequence is identical in human, mouse, bovine and porcine. View All» Species Reactivity: Rat View All» Application Notes: Western blot: 1:2000 using ECL on rat brain lysate. Dilutions should be made using a carrier protein such as BSA (1-3%) Optimal working dilutions must be determined by the end user. SUGGESTED WESTERN BLOT PROTOCOL 1. Mix the samples (organ membranes: 50 μg/lane; transfected cells: 500,000 cells/lane) with sample-buffer X 2, and heat 10 min at 70°C. 2. 5-50 μL applied to Minigel lane (0.75-1.5 mm width) and run at standard conditions. (60 mA for 2 1.5 mm Minigel gels, 1.4 h). It is suggested that you run 5-15% acrylamide (37.5:1 acrylamide:bisacrysmide) minigel (1.5 mm width) at 30 mA/gel ~1-1.5 hours. 3. Transfer in semi-dry system under standard conditions (3 h 100 mA for two minigel gels) 4. Stain the transferred bands with Chemicon BLOT-FastStain (Catalog Number 2076). 5. Destain with deionized water. 6. Block with 5% non-fat milk (Marvel or Carnation) in PBS, and 0.025 % sodium azide, overnight at 2-8°C. The non-fat milk should be dissolved freshly, centrifuged 10,000 rpm for 10 min, and filtered through glass filter (Gelman Acrodisc). 7. Incubation with first antibody 2 h at room temperature or overnight at 4°C in blocking solution. The antibody preparation should be centrifuged before use (10,000 g 5 min.). Optimal working dilutions and incubation time will need to be determined by the end user. 8. Wash 4 x 10 min. with PBS-0.1% tween 20. From this stage, azide should be omitted. 9. Incubation with the secondary antibody (HRP-conjugated goat anti-rabbit antibody, for example Chemicon Catalog Number AP132P, diluted appropriately) 1 h at room temperature. 10. Wash 4 x 10 min. with PBS-0.1% tween 20. 11. Perform ECL with commercial kits (Chemilucent, Chemicon Catalog Number 2600). View All» Control: Included free of charge with the antibody is 40 μg of control antigen. The stock solution of the antigen can be made up using 100 μL of sterile distilled water. For negative control, preincubate 1 μg of peptide with 1 μg of antibody for one hour at room temperature. Optimal concentrations must be determined by the end user. View All» Presentation: Affinity purified immunoglobulin. Lyophilized from phosphate buffered saline, pH 7.4, containing 1% BSA, and 0.05% sodium azide as a preservative. Reconstitute with 50 μL of sterile deionized water. Centrifuge antibody preparation before use (10,000 xg for 5 min). View All» Storage Conditions: Maintain lyophilized material at -20°C for up to 12 months after date of receipt. After reconstitution maintain at -20°C in undiluted aliquots for up to 6 months. Avoid repeated freeze/thaw cycles. View All» UniProt Number: P20309 View All» Entrez Gene Number: NM_000740.2 View All» Gene Symbol:

• CHRM3

• HM3
  View All» Usage Statement: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. View All» Key Applications: Western Blotting View All» Entrez Gene Summary: The muscarinic cholinergic receptors belong to a larger family of G protein-coupled receptors. The functional diversity of these receptors is defined by the binding of acetylcholine and includes cellular responses such as adenylate cyclase inhibition, phosphoinositide degeneration, and potassium channel mediation. Muscarinic receptors influence many effects of acetylcholine in the central and peripheral nervous system. The muscarinic cholinergic receptor 3 controls smooth muscle contraction and its stimulation causes secretion of glandular tissue. View All» UniProt Summary: FUNCTION: SwissProt: P20309 # The muscarinic acetylcholine receptor mediates various cellular responses, including inhibition of adenylate cyclase, breakdown of phosphoinositides and modulation of potassium channels through the action of G proteins. Primary transducing effect is Pi turnover.SIZE: 590 amino acids; 66128 Da SUBCELLULAR LOCATION: Basolateral cell membrane; Multi-pass membrane protein.SIMILARITY: SwissProt: P20309 ## Belongs to the G-protein coupled receptor 1 family. View All» Brand Family: Chemicon View All» Product Name: Anti-Muscarinic Acetylcholine Receptor m3 Antibody | AB9018-50UL View All» Concentration: 0.8 mg/mL (after reconstitution) View All» Antibody Type: Polyclonal Antibody View All» Qty/Pk: 50 µL View All» Format: Affinity Purified View All» Host: Rabbit View All»
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