货号：AB902

品牌：Merck Millipore

规格：

目录价：￥3732.00

市场价格：￥3172.20

会员价格：￥2985.60

金山科研平台，产品价格货期咨询微信：jinshanbio Description: Anti-Adrenocorticotropic Hormone Antibody | AB902 View All» Promotional Text: Special Shipping Offer on Antibodies100% Performance Guaranteed View All» Trade Name: Chemicon (Millipore) View All» Specificity: The antiserum provided has been evaluated for its ability to give a specific and positive immunostaining of ACTH-containing cells using both the immunoperoxidase and immunofluoresence procedures. View All» Background Information: ANTIGEN: Synthetic ACTH (1-24) bound to keyhole limpet hemocyanin (KLH) with carbodiimide. View All» Species Reactivity:

• Bovine

• Canine

• Human

• Rat

• Sheep

• Feline
  View All» Application Notes: TISSUE EVALUATION:Formalin-fixed and paraffin-embedded sections (6 microns thick) of anterior pituitary glands were prepared from a number of species including man, dog, rat, cat, ox and sheep. Cryostat sections of the above tissued can also be used in the staining procedures.IMMUNOPEROXIDASESTAINING:1. The sections to be stained are hydrated to phosphate buffered saline (PBS, pH 7.4).2. The antiserum diluted 1:1,000 - 1:2,000 (in PBS) is allowed to react with the tissue at 37° C for 30 minutes.3.Using PBS, the tissue is washed for 15 min at room temperature and treated sequentially with sheep anti-rabbit gamma globulin (diluted 1:20 in PBS) and peroxidase anti-peroxidase complex (diluted 1:100 in PBS) for 37°C. After each of these two steps excess reagent is washed from the section as described above.4. The final step in the staining involves treating the section with a mixture of 3,3'- diaminbenzidine HCl (0.02%) in hydrogen peroxide (0.004%). For preservation, the sections are dehydrated, cleared and mounted.IMMUNOFLUORESCENCESTAINING:1. Same as Step 1 above.2. The tissue is reacted with the antiserum, diluted 1:20-1:40 in (PBS) for 30 minutes at 37°C.3. After washing off the excess reagents with PBS, the section is treated with fluoresceinlabeled sheep anti-rabbit gamma globulin (diluted 1:10 in PBS) for 30 min at 37° C. The tissue is washed with PBS and after mounting under glycerine-saline is viewed with a fluorescence microscope.COMMENTS:The antiserum dilutions described have been established as giving a positive staining on the corticotrophs using the above protocol. It is recommended, however, that the customer establishes his own optimal dilutions to give positive staining. In general, staining can occur on further dilution of the primary antiserum if this is accompanied by an increase in its incubation time e.g. 24 hr. View All» Presentation: Liquid. Contains 0.1% sodium azide. View All» Storage Conditions: Store at -20°C in undiluted aliquots for up to 12 months. Do not thaw and refreeze material. View All» UniProt Number: P01189 View All» Entrez Gene Number:
  • NM_000939.2

  • NM_001035256.1
    View All» Gene Symbol:
    • POMC

    • pro-ACTH-endorphin

    • adrenocorticotropin

    • beta-LPH

    • POC

    • gamma-LPH

    • LPH

    • ACTH

    • corticotropin

    • beta-endorphin

    • beta-MSH

    • alpha-MSH

    • pro-opiomelanocortin

    • proopiomelanocortin

    • Pro-opiomelanocortin

    • corticotropin-lipotrophin

    • NPP

    • MSH

    • met-enkephalin

    • CLIP

    • gamma-MSH
      View All» Alternate Names: ACTH View All» Usage Statement: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. View All» Key Applications: Immunohistochemistry (Paraffin) View All» Entrez Gene Summary: This gene encodes a polypeptide hormone precursor that undergoes extensive, tissue-specific, post-translational processing via cleavage by subtilisin-like enzymes known as prohormone convertases. There are eight potential cleavage sites within the polypeptide precursor and, depending on tissue type and the available convertases, processing may yield as many as ten biologically active peptides involved in diverse cellular functions. The encoded protein is synthesized mainly in corticotroph cells of the anterior pituitary where four cleavage sites are used; adrenocorticotrophin, essential for normal steroidogenesis and the maintenance of normal adrenal weight, and lipotropin beta are the major end products. In other tissues, including the hypothalamus, placenta, and epithelium, all cleavage sites may be used, giving rise to peptides with roles in pain and energy homeostasis, melanocyte stimulation, and immune modulation. These include several distinct melanotropins, lipotropins, and endorphins that are contained within the adrenocorticotrophin and beta-lipotropin peptides. Mutations in this gene have been associated with early onset obesity, adrenal insufficiency, and red hair pigmentation. Alternatively spliced transcript variants encoding the same protein have been described. View All» UniProt Summary: FUNCTION: SwissProt: P01189 # Beta-endorphin and Met-enkephalin are endogenous opiates.SIZE: 267 amino acids; 29424 Da TISSUE SPECIFICITY: ACTH and MSH are produced by the pituitary gland.PTM: Specific enzymatic cleavages at paired basic residues yield the different active peptides. & O-glycosylated; reducing sugar is probably N- acetylgalactosamine.DISEASE: SwissProt: P01189 # Defects in POMC may be associated with susceptibility to obesity [MIM:601665]. & Defects in POMC are the cause of pro-opiomelanocortinin deficiency [MIM:609734]. Affected individuals present early-onset obesity, adrenal insufficiency and red hair.SIMILARITY: SwissProt: P01189 ## Belongs to the POMC family. View All» Brand Family: Chemicon View All» Product Name: Anti-Adrenocorticotropic Hormone Antibody | AB902 View All» Antibody Type: Polyclonal Antibody View All» Qty/Pk: 500 µL View All» Format: Serum View All» Host: Rabbit View All»
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