货号：AB924

品牌：Merck Millipore

规格：EA

目录价：询价

金山科研平台，产品价格货期咨询微信：jinshanbio Description: Anti-Estradiol View All» Promotional Text: Special Shipping Offer on Antibodies100% Performance Guaranteed View All» Trade Name: Chemicon (Millipore) View All» Specificity: The antiserum provided has been evaluated for its ability to give a positive and specific immunostaining of estradiol-containing cells in human mammary gland carcinomas and Sertoli-Leydig tumors of the ovary using both the immunoperoxidase and immunofluoresence procedures. View All» Immunogen: Estradiol-BSA. View All» Species Reactivity: All View All» Application Notes: TISSUE EVALUATION: Formalin-fixed and paraffin-embedded sections (6 microns thick) of human tumor tissue were prepared. Cryostat sections of the above tissues can also be used. IMMUNOPEROXIDASE STAINING: 1. The sections to be stained are hydrated to phosphate buffered saline (PBS, pH 7.4). 2.The antiserum diluted 1:150-300 (in PBS) is allowed to react with the tissue at 37°C for 30 minutes. 3.Using PBS, the tissue is washed for 15 minutes at room temperature and treated sequentially with goat anti-rabbit gamma globulin (diluted 1:20 in PBS) and peroxidase anti-peroxidase complex (diluted 1:100 in PBS) for 37°C. After each of these two steps excess reagent is washed from the section as described above. 4.The final step in the staining involves treating the section with a mixture of 3,3'-diaminbenzidine HCl (0.02%) in hydrogen peroxide (0.004%). For preservation, the sections are dehydrated, cleared and mounted. IMMUNOFLUORESCENCE STAINING: 1.Same as Step 1 above. 2.The tissue is reacted with the antiserum, diluted 1:20-1:40 in (PBS) for 30 minutes at 37°C. 3.After washing off the excess antiserum with PBS, the section is treated with fluorescein labeled goat anti-rabbit gamma globulin (diluted 1:10 in PBS) for 30 minutes at 37°C. The tissue is washed with PBS and after mounting under glycerine-saline is viewed with a fluorescence microscope. COMMENTS: The antiserum dilutions described have been established as giving a positive staining of estradiol-containing cells using the above protocol. It is recommended, however, that the customer establishes his own optimal dilutions to give positive staining. In general, staining can occur on further dilution of the primary antiserum if this is accompanied by an increase in its incubation time e.g. 24 hr. View All» Presentation: Liquid rabbit serum. View All» Storage Conditions: Maintain at -20°C in undiluted aliquots for up to 12 months. Avoid repeated freeze/thaw cycles. View All» Usage Statement: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. View All» Key Applications: Immunohistochemistry (Paraffin) View All» Brand Family: Chemicon View All» Product Name: Anti-Estradiol View All» Antibody Type: Polyclonal Antibody View All» Qty/Pk: 1 mL View All» Format: Serum View All» Host: Rabbit View All»