货号：AB932

品牌：Merck Millipore

规格：

目录价：￥4692.00

市场价格：￥3988.20

会员价格：￥3753.60

金山科研平台，产品价格货期咨询微信：jinshanbio Description: Anti-Glucagon Antibody | AB932 View All» Promotional Text: Special Shipping Offer on Antibodies100% Performance Guaranteed View All» Trade Name: Chemicon (Millipore) View All» Specificity: The antiserum supplied has been evaluated for its ability to stain cells in the pancreas containing glucagon-like immunoreactivity using both the immunoperoxidase and immunofluorescence procedures. View All» Background Information: ANTIGEN: Synthetic glucagon bound to keyhold limpet hemocyanin (KLH) with carbodiimide. View All» Species Reactivity:

• Canine

• Rat

• Sheep

• Feline

• Ox
  View All» Application Notes: TISSUE EVALUATION:Formalin-fixed and paraffin embedded sections (6 microns thick) were prepared from a number of species including rat, cat, dog, ox and sheep. Cryostat sections of the above tissues can also be used in the staining procedures.IMMUNOPEROXIDASESTAINING:1. The sections to be stained are hydrated to phosphated buffered saline (PBS, pH 7.4).2. The antiserum diluted 1:500-1:1000 (in PBS) is allowed to react with the tissue at 37°C for 30 minutes.3. Using PBS, the tissue is washed for 15 minutes at room temperature and treated sequentially with goat anti-rabbit gamma globulin (diluted 1:20 in PBS) and peroxidase anti-peroxidase complex (diluted 1:100 in PBS) for 30 minutes at 37°C. After each of these steps, the tissue is washed with PBS as before.4. The final stage of the reaction is treatment of the section with a mixture of 3,3' -diaminbenzidine HCI (0.02%) in hydrogen peroxide (0.004%). For preservation, the sections are dehydrated, cleared and mounted.IMMUNOFLUORESCENCESTAINING:1. Same as Step 1 above.2. The tissue is reacted with the antiserum, diluted 1:20-1:40 in (PBS) for 30 minutes at 37°C.3. After washing off the excess antiserum, with PBS, the section is treated with fluorescein labeled goat anti-rabbit gamma globulin serum (diluted 1:10 in PBS) for 30 minutes at 37°C. The tissue is washed with PBS and after mounting under saline-glycerol, is viewed with a fluorescence microscope.COMMENTS:The antiserum described here has been established to give a positive staining of glucagon cells using the above protocols. It is recommended, however, that the customer establishes his own optimal dilutions to give positive staining. In general, staining can occur on further dilution of the primary antiserum is this is accompanied by an increase in its incubation time, e.g. 24 hours at either 37°C or 4°C. View All» Presentation: Liquid rabbit antiserum containing 0.1% sodium azide. View All» Storage Conditions: Maintain at -20°C in undiluted aliquots for up to 12 months. Avoid repeated freeze/thaw cycles. View All» UniProt Number: P01275 View All» Entrez Gene Number: NM_002054.2 View All» Gene Symbol:
  • GCG

  • GRPP

  • GLP1

  • GLP2

  • glucagon
    View All» Usage Statement: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. View All» Key Applications: Immunohistochemistry (Paraffin) View All» Entrez Gene Summary: The protein encoded by this gene is actually a preproprotein that is cleaved into four distinct mature peptides. One of these, glucagon, is a pancreatic hormone that counteracts the glucose-lowering action of insulin by stimulating glycogenolysis and gluconeogenesis. Glucagon is a ligand for a specific G-protein linked receptor whose signalling pathway controls cell proliferation. Two of the other peptides are secreted from gut endocrine cells and promote nutrient absorption through distinct mechanisms. Finally, the fourth peptide is similar to glicentin, an active enteroglucagon. View All» UniProt Summary: FUNCTION: SwissProt: P01275 # Glicentin may modulate gastric acid secretion and the gastro-pyloro-duodenal activity. May play an important role in intestinal mucosal growth in the early period of life.SIZE: 180 amino acids; 20909 Da SUBCELLULAR LOCATION: Secreted.TISSUE SPECIFICITY: Glucagon is secreted in the A cells of the islets of Langerhans. GLP-1, GLP-2, oxyntomodulin and glicentin are secreted from enteroendocrine cells throughout the gastrointestinal tract. GLP1 and GLP2 are also secreted in selected neurons in the brain.PTM: Proglucagon is post-translationally processed in a tissue- specific manner in pancreatic A cells and intestinal L cells. In pancreatic A cells, the major bioactive hormone is glucagon cleaved by PCSK2/PC2. In the intestinal L cells PCSK1/PC1 liberates GLP-1, GLP-2, glicentin and oxyntomodulin. GLP-1 is further N-terminally truncated by post-translational processing in the intestinal L cells resulting in GLP-1(7-37) GLP-1-(7-36)amide. The C-terminal amidation is neither important for the metabolism of GLP-1 nor for its effects on the endocrine pancreas.SIMILARITY: SwissProt: P01275 ## Belongs to the glucagon family.MISCELLANEOUS: In the glucagon antagonist, His-53 and Phe-58 are missing. This antagonist has been successfully utilized to reduce glucose concentration in vivo." View All» Brand Family: Chemicon View All» Product Name: Anti-Glucagon Antibody | AB932 View All» Antibody Type: Polyclonal Antibody View All» Qty/Pk: 500 µL View All» Format: Serum View All» Host: Rabbit View All»
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