货号：AB936

品牌：Merck Millipore

规格：

目录价：￥3510.00

市场价格：￥2983.50

会员价格：￥2808.00

金山科研平台，产品价格货期咨询微信：jinshanbio Description: Anti-Chorionic Gonadotropin View All» Promotional Text: Special Shipping Offer on Antibodies100% Performance Guaranteed View All» Trade Name: Chemicon (Millipore) View All» Specificity: The antiserum supplied has been evaluated for its ability to give a positive and specific immunostaining of syncytial cells in the immature human placenta using both the immunoperoxidase and immunofluorescence procedures. View All» Immunogen: Purified chorionic gonadotropin (HCG) extracted from human placentas and pregnancy urine. View All» Species Reactivity: Human View All» Application Notes: TISSUE EVALUATION: Formalin fixed and paraffin embedded sections (6 microns thick) were prepared from first trimester human placentas. IMMUNOPEROXIDASE STAINING: 1. The sections to be stained are hydrated to phosphated buffered saline (PBS, pH 7.4). 2.The antiserum diluted 1:250 (in PBS) is allowed to react with the tissue at 37°C for 30 minutes. 3.Using PBS, the tissue is washed for 15 minutes at room temperature and treated sequentially with goat anti-rabbit gamma globulin (diluted 1:20 in PBS) and peroxidase anti-peroxidase complex (diluted 1:100 in PBS) for 30 minutes at 37°C. After each of these steps, the tissue is washed with PBS as before. 4.The final stage of the reaction is treatment of the sections with a mixture of 3,3'-diamin-benzidine HCl (0.02%) in hydrogen peroxide (0.004%). For preservation, the sections are dehydrated, cleared and mounted. IMMUNOFLUORESCENCE STAINING: 1.Same as Step 1 above. 2.The tissue is reacted with the antiserum, diluted 1:20-1:40 in (PBS) for 30 minutes at 37°C. 3.After washing off the excess antiserum with PBS, the section is treated with fluorescein labeled goat anti-rabbit gamma globulin serum (diluted 1:10 in PBS) for 30 minutes at 37°C. The tissue is washed with PBS and after mounting under saline gylcerol, is viewed with a fluorescence microscope. COMMENTS: The antiserum described here has been established to give a positive staining of the hormone containing cells using the above protocols. It is recommended, however, that the customer establishes his own optimal dilutions to give positive staining. In general, staining can occur on further dilution of the primary antiserum if this is accompanied by an increase in its incubation time e.g. 24 hours at either 37°C or 2-8°C. View All» Presentation: Rabbit serum. Liquid. Contains 0.1% sodium azide. View All» Storage Conditions: Maintain at -20ºC in undiluted aliquots for up to 12 months. Avoid repeated freeze/thaw cycles. View All» UniProt Number: P01233 View All» Entrez Gene Number: NM_000737.2 View All» Gene Symbol:

• CGB

• CGB7

• hCGB

• CGB8

• CGB3

• CGB5

• CG-beta
  View All» Alternate Names: hCG View All» Usage Statement: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. View All» Key Applications: Immunohistochemistry (Paraffin) View All» Entrez Gene Summary: This gene is a member of the glycoprotein hormone beta chain family and encodes the beta 3 subunit of chorionic gonadotropin (CG). Glycoprotein hormones are heterodimers consisting of a common alpha subunit and an unique beta subunit which confers biological specificity. CG is produced by the trophoblastic cells of the placenta and stimulates the ovaries to synthesize the steroids that are essential for the maintenance of pregnancy. The beta subunit of CG is encoded by 6 genes which are arranged in tandem and inverted pairs on chromosome 19q13.3 and contiguous with the luteinizing hormone beta subunit gene. View All» UniProt Summary: FUNCTION: SwissProt: P01233 # Stimulates the ovaries to synthesize the steroids that are essential for the maintenance of pregnancy.SIZE: 165 amino acids; 17739 Da SUBUNIT: Heterodimer of a common alpha chain and a unique beta chain which confers biological specificity to thyrotropin, lutropin, follitropin and gonadotropin.SUBCELLULAR LOCATION: Secreted.TISSUE SPECIFICITY: Placenta.DEVELOPMENTAL STAGE: Made by the first trimester placenta.SIMILARITY: SwissProt: P01233 ## Belongs to the glycoprotein hormones subunit beta family. View All» Brand Family: Chemicon View All» Product Name: Anti-Chorionic Gonadotropin View All» Antibody Type: Polyclonal Antibody View All» Qty/Pk: 500 µL View All» Format: Serum View All» Host: Rabbit View All»
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