货号:AB938
品牌:Merck Millipore
规格:
目录价:¥4095.00
市场价格:¥3480.75
会员价格:¥3276.00
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Description:
Anti-Serotonin Antibody | AB938
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Promotional Text:
Special Shipping Offer on Antibodies100% Performance Guaranteed
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Trade Name:
Chemicon (Millipore)
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Specificity:
The antiserum provided has been evaluated for its ability to stain serotonin-containing structures in the ileum of man. Staining is positive using both the immunoperoxidase or immunofluorescence procedures.
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Immunogen:
The antigen used in the production of this antiserum is serotonin covalently bound to bovine thyroglobulin with carbodiimide.
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Species Reactivity:
Human
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Application Notes:
TISSUE EVALUATION:Formalin-fixed and paraffin embedded sections (5-6 microns thick) of human small intestine were used in the evaluation of this antiserum.IMMUNOPEROXIDASESTAINING: 1. The sections to be stained are hydrated to phosphate buffered saline (PBS, pH 7.4).2. The antiserum diluted 1:1,000-1:2,000 (in PBS) is allowed to react with the tissue at 37*C for 30 minutes.3. Using PBS, the tissue is washed for 15 minutes at room temperature and treated sequentially with goat anti-rabbit gamma globulin (diluted 1:20 in PBS) and peroxidase anti-peroxidase complex (diluted 1:100 in PBS) for 30 minutes at 37*C. After each of these steps, the tissue is washed with PBS as before.4. The final stage of the reaction is treatment of the section with a mixture of 3,3' -diamin-benzidine HCl (0.02%) in hydrogen peroxide (0.004%). For preservation, the sections are dehydrated, cleared and mounted.IMMUNOFLUORESCENCESTAINING:1. Same as Step 1 above.2. The tissue is reacted with the antiserum, diluted 1:20-1:40 in (PBS) for 30 minutes at 37*C.3. After washing off the excess antiserum with PBS, the section is treated with fluoroscein-labeled goat anti-rabbit gamma globulin serum (diluted 1:10 in PBS) for 30 minutes at 37*C. The tissue is washed with PBS and after mounting under saline-glycerol, is viewed with a fluorescence microscope.
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Presentation:
Liquid antiserum. Contains 0.1% sodium azide.
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Storage Conditions:
Maintain at -20°C in undiluted aliquots for up to 12 months. Avoid repeated freeze/thaw cycles.
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Alternate Names:
5-HT; 5-Hydroxytryptamine
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Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Key Applications:
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Immunofluorescence
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Immunohistochemistry (Paraffin)
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Brand Family:
Chemicon
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Product Name:
Anti-Serotonin Antibody | AB938
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Antibody Type:
Polyclonal Antibody
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Qty/Pk:
500 µL
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Target / Localization:
Presynaptic
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Format:
Serum
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Host:
Rabbit
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