货号：AB941

品牌：Merck Millipore

规格：

目录价：￥3861.00

市场价格：￥3281.85

会员价格：￥3088.80

金山科研平台，产品价格货期咨询微信：jinshanbio Description: Anti-S-100 Protein Antibody | AB941 View All» Replaces: CBL410 View All» Promotional Text: Special Shipping Offer on Antibodies100% Performance Guaranteed View All» Trade Name: Chemicon (Millipore) View All» Specificity: The antiserum supplied has been evaluated for its ability to give a positive and specific immunostaining of S-100-containing cells present in normal skin and human neoplasms using both the immunoperoxidase and immunofluorescence procedures. View All» Immunogen: Purified bovine S-100 bound to keyhole limpet hemocyanin (KLH) with carbodiimide. View All» Species Reactivity:

• Human

• Rat
  View All» Application Notes: TISSUE EVALUATION: Formalin-fixed and paraffin embedded sections (6 microns thick) of human skin and squamous cell carcinomas were prepared. Cryostat sections of the above tissues may also be used. IMMUNOPEROXIDASE STAINING: 1. The sections to be stained are hydrated with phosphate buffered saline (PBS), pH 7.4. 2. The antiserum diluted 1:250-1:500 (in PBS) is allowed to react with the tissue at 37*C for 30 minutes. 3. Using PBS, the tissue is washed for 15 minutes at room temperature and treated sequentially with goat anti-rabbit gamma globulin (diluted 1:20 in PBS) and peroxidase anti-peroxidase complex (diluted 1:100 in PBS) for 30 minutes at 37*C. After each of these steps, the tissue is washed with PBS as before. 4. The final stage of the reaction is treatment of the section with a mixture of 3,3'-diaminbenzidine HCl (0.02%) in hydrogen peroxide (0.004%). For preservation, the sections are dehydrated, cleared and mounted. IMMUNOFLUORESCENCE STAINING: 1. Same as Step 1 above. 2. The tissue is reacted with the antiserum, diluted 1:20-1:40 in (PBS) for 30 minutes at 37*C. 3. After washing off the excess reagents with PBS, the section is treated wit fluorescein-labeled goat anti-rabbit gamma globulin serum (diluted 1:10 in PBS) for 30 minutes at 37*C. The tissue is washed with PBS and after mounting under saline-glycerol, is viewed with a fluorescence microscope. COMMENTS: The antiserum described here has been established to give a positive staining of the S-100 cells using the above protocols. It is recommended, however, that the customer establishes his own optimal dilutions to give positive staining. In general, staining can occur on further dilution of the primary antiserum if this is accompanied by an increase in the incubation time, e.g. 24 hours. View All» Presentation: Rabbit serum. Liquid. Contains 0.1% sodium azide. View All» Storage Conditions: Maintain at -20°C in undiluted aliquots for up to 12 months. Avoid repeated freeze/thaw cycles. View All» UniProt Number:
  • P23297

  • P04271
    View All» Entrez Gene Number: NM_006271.1 View All» Gene Symbol:
    • S100A1

    • S100A

    • S100

    • S100-alpha
      View All» Usage Statement: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. View All» Key Applications:
      • Immunohistochemistry

      • Immunohistochemistry (Paraffin)
        View All» Entrez Gene Summary: The protein encoded by this gene is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21. This protein may function in stimulation of Ca2+-induced Ca2+ release, inhibition of microtubule assembly, and inhibition of protein kinase C-mediated phosphorylation. Reduced expression of this protein has been implicated in cardiomyopathies. View All» UniProt Summary: FUNCTION: SwissProt: P23297 # Weakly binds calcium but binds zinc very tightly- distinct binding sites with different affinities exist for both ions on each monomer. Physiological concentrations of potassium ion antagonize the binding of both divalent cations, especially affecting high-affinity calcium-binding sites.SIZE: 94 amino acids; 10546 Da SUBUNIT: Dimer of either two alpha chains, or two beta chains, or one alpha and one beta chain.SUBCELLULAR LOCATION: Cytoplasm.TISSUE SPECIFICITY: Highly prevalent in heart. Also found in lesser quantities in skeletal muscle and brain.SIMILARITY: SwissProt: P23297 ## Belongs to the S-100 family. & Contains 2 EF-hand domains. View All» Brand Family: Chemicon View All» Product Name: Anti-S-100 Protein Antibody | AB941 View All» Antibody Type: Polyclonal Antibody View All» Qty/Pk: 100 µL View All» Format: Serum View All» Host: Rabbit View All»
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