货号：AB951

品牌：Merck Millipore

规格：EA

目录价：询价

金山科研平台，产品价格货期咨询微信：jinshanbio Description: Anti-Neuron Specific Enolase Antibody | AB951 View All» Promotional Text: Special Shipping Offer on Antibodies100% Performance Guaranteed View All» Trade Name: Chemicon (Millipore) View All» Specificity: AB951 stains many neuronal cell bodies and processes within the central and peripheral nervous system. View All» Molecular Weight: ~39 kDa View All» Immunogen: Synthetic peptide obtained from hNSE. View All» Background Information: The glycolytic enzyme enolase (2-phospho-D-glycerate hydrolase, E.C. 4.2.1.11) is composed of two subunits and exists in three molecular forms in human brain: non-neuronal enolase (NNE; alpha, alpha'-enolase), neuron-specific enolase (NSE; gamma,gamma'-enolase, also known as 14-3-2 protein and NSP)(Grasso et al. 1977; Schmechel et al. 1979) and the short-lived heterodimer alpha, gamma'-enolase. The neuron specific form is a highly acidic homodimeric protin of 78kDa (Schmechel et al. 1978), which has been found in central and peripheral nervous elements and also in many neuroendocrine cells and derivative tumors (Marangos 1985). NSE is regarded as the best described serum marker for small cell lung cancer. In addition, the measurement of NSE levels in both serum and cerebrospinal fluid following cerebral ischaemia and traumatic head injury provides a reliable laboratory indicator of the degree of brain cell damage and may allow early prediction of prognostic outcome (DeGiorgio et al. 1995; Yamazaki et al. 1995). View All» Species Reactivity:

• Bovine

• Rat
  View All» Application Notes: Western Blot: 1:100-1:1,000 (ECL) On denatured Western blots of purified bovine NSE the antibody recognizes a single band at approximately 39 kDa. On Western blots of whole rat brain lysates (non-reduced) a more complex series of bands is observed ranging from 78/80 kDa (NSE homodimer) to 20 kDa (gamma-enolase degradation product). Immunohistochemistry: 1:100-1:1,000 (ABC) The antibody has been used successfully on formaldehyde-fixed cryostat, Vibratome and de-paraffinized tissues. For indirect immunofluorescence procedures use at a dilution of 1:80-1:160. Dilute to working concentration with 50 mM PBS, pH 7.4 containing 1.5% NaCl and 1% Normal Goat Serum (if a goat anti-rabbit IgG linker antibody is to be used). Optimal working dilutions must be determined by the end user. IMMUNOHISTOCHEMISTRY PROTOCOL FOR AB951 This antibody has been used successfully on 30 μm, free floating, 4% paraformaldehyde fixed rat brain tissue. All steps are performed under constant agitation. Suggested protocol follows. 1) 3 x 10 minute washes in TBS (without Triton). 2) Incubate for 30 minutes in TBS with 3% serum (same as host from secondary antibody) & Triton (must be optimized, start @ 0.1%). 3) Incubate primary antibody diluted appropriately in TBS with 1% serum (same as host from secondary antibody) (without Triton) for 2 hours at room temperature followed by 16 hours at 4°C. 4) 3 x 10 minute washes in TBS. 5) Incubate with secondary antibody diluted appropriately in TBS with 1% serum (same as host from secondary antibody). 6) 3 x 10 minute washes in TBS. 7) ABC Elite (1:200 Vector Labs) in TBS. 8) 2 x 10 minute washes in TBS. 9) 1 x 10 minute wash in phosphate buffer (no saline). 10) DAB reaction with 0.06% NiCl added for intensification. 11) 2 x 10 minute washes in PBS. 12) 1 x 10 minute wash in phosphate buffer (no saline). View All» Control: Brain tissue View All» Purification Method: Unpurified View All» Presentation: Rabbit antiSerum. Liquid in 0.1M NaCl. Contains 0.1% sodium azide. Dilute if necessary, with 50 mM PBS, pH 7.4 containing 1.5% NaCl and 1% Normal Goat Serum (if a goat anti-rabbit IgG linker antibody is to be used). View All» Storage Conditions: Maintain for 1 year at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. View All» UniProt Number: P09104 View All» Entrez Gene Number: NM_001975.2 View All» Gene Symbol:
  • ENO2

  • NSE

  • Gamma-enolase

  • EC 4.2.1.11
    View All» Alternate Names: NSE; gamma Enolase; Neural Enolase; Enolase 2 View All» Usage Statement: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. View All» Key Applications:
    • Immunohistochemistry

    • Immunohistochemistry (Paraffin)

    • Western Blotting
      View All» Entrez Gene Summary: This gene encodes one of the three enolase isoenzymes found in mammals. This isoenzyme, a homodimer, is found in mature neurons and cells of neuronal origin. A switch from alpha enolase to gamma enolase occurs in neural tissue during development in rats and primates. View All» UniProt Summary: FUNCTION: SwissProt: P09104 # Has neurotrophic and neuroprotective properties on a broad spectrum of central nervous system (CNS) neurons. Binds, in a calcium-dependent manner, to cultured neocortical neurons and promotes cell survival (By similarity).COFACTOR: Magnesium. Required for catalysis and for stabilizing the dimer.SIZE: 434 amino acids; 47269 Da SUBUNIT: Mammalian enolase is composed of 3 isozyme subunits, alpha, beta and gamma, which can form homodimers or heterodimers which are cell-type and development-specific.SUBCELLULAR LOCATION: Cytoplasm (By similarity). Cell membrane (By similarity). Note=Can translocate to the plasma membrane in either the homodimeric (alpha/alpha) or heterodimeric (alpha/gamma) form (By similarity).TISSUE SPECIFICITY: The alpha/alpha homodimer is expressed in embryo and in most adult tissues. The alpha/beta heterodimer and the beta/beta homodimer are found in striated muscle, and the alpha/gamma heterodimer and the gamma/gamma homodimer in neurons.DEVELOPMENTAL STAGE: During ontogenesis, there is a transition from the alpha/alpha homodimer to the alpha/beta heterodimer in striated muscle cells, and to the alpha/gamma heterodimer in nerve cells.SIMILARITY: SwissProt: P09104 ## Belongs to the enolase family. View All» Brand Family: Chemicon View All» Product Name: Anti-Neuron Specific Enolase Antibody | AB951 View All» Concentration: Variable View All» Antibody Type: Polyclonal Antibody View All» Qty/Pk: 500 μL View All» Format: Serum View All» Host: Rabbit View All»
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