货号：AB9582-200UL

品牌：Merck Millipore

规格：

目录价：￥8096.00

市场价格：￥6881.60

会员价格：￥6476.80

金山科研平台，产品价格货期咨询微信：jinshanbio Description: Anti-Somatostatin Receptor Type 4 Antibody | AB9582-200UL View All» Promotional Text: Special Shipping Offer on Antibodies100% Performance Guaranteed View All» Trade Name: Chemicon (Millipore) View All» Specificity: Recognizes Somatostatin Receptor Type 4 (SSTR4). View All» Immunogen: Purified peptide from amino acids 182-194 of rat SSTR4 (Accession number P30937). The immunogen sequence is identical in mouse and 12/13 identical in human. View All» Species Reactivity: Rat View All» Application Notes: Western blot: 1:200 using ECL on rat brain lysate. Antibody recognizes a single band around 40 kDa. Dilutions should be made using a carrier protein such as BSA (1-3%) Optimal working dilutions must be determined by the end user. SUGGESTED WESTERN BLOT PROTOCOL 1. Mix the samples (organ membranes: 50 μg/lane; transfected cells: 500,000 cells/lane) with sample-buffer X 2, and heat 10 min at 70°C. 2. 5-50 μL applied to Minigel lane (0.75-1.5 mm width) and run at standard conditions. (60 mA for 2 1.5 mm Minigel gels, 1.4 h). It is suggested that you run 5-15% acrylamide (37.5:1 acrylamide:bisacrysmide) minigel (1.5 mm width) at 30 mA/gel ~1-1.5 hours. 3. Transfer in semi-dry system under standard conditions (3 h 100 mA for two minigel gels) 4. Stain the transferred bands with Chemicon BLOT-FastStain (Catalog Number 2076). 5. Destain with deionized water. 6. Block with 5% non-fat milk (Marvel or Carnation) in PBS, and 0.025 % sodium azide, overnight at 2-8°C. The non-fat milk should be dissolved freshly, centrifuged 10,000 rpm for 10 min, and filtered through glass filter (Gelman Acrodisc). 7. Incubation with first antibody 2 h at room temperature or overnight at 4°C in blocking solution. The antibody preparation should be centrifuged before use (10,000 g 5 min.). Optimal working dilutions and incubation time will need to be determined by the end user. 8. Wash 4 x 10 min. with PBS-0.1% tween 20. From this stage, azide should be omitted. 9. Incubation with the secondary antibody (HRP-conjugated goat anti-rabbit antibody, for example Chemicon Catalog Number AP132P, diluted appropriately) 1 h at room temperature. 10. Wash 4 x 10 min. with PBS-0.1% tween 20. 11. Perform ECL with commercial kits (Chemilucent, Chemicon Catalog Number 2600). View All» Control: Included free of charge with the antibody is 40 μg of control antigen (lyophilized powder). The stock solution of the antigen can be made up using 100 μL of sterile deionized water. For negative control, preincubate 1 μg of peptide with 1 μg of antibody for one hour at room temperature. Optimal concentrations must be determined by the end user. View All» Presentation: Affinity purified immunoglobulin. Lyophilized from phosphate buffered saline, pH 7.4, containing 1% BSA, and 0.05% sodium azide as a preservative. Reconstitute with 200 μL of sterile deionized water. Centrifuge antibody preparation before use (10,000 xg for 5 min). View All» Storage Conditions: Maintain lyophilized material at -20°C for up to 12 months after date of receipt. After reconstitution maintain at -20°C in undiluted aliquots for up to 6 months. Avoid repeated freeze/thaw cycles. View All» UniProt Number: P31391 View All» Entrez Gene Number: NM_001052.2 View All» Gene Symbol:

• SSTR4

• SS4R
  View All» Alternate Names: SSTR4 View All» Usage Statement: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. View All» Key Applications: Western Blotting View All» Entrez Gene Summary: Somatostatin acts at many sites to inhibit the release of many hormones and other secretory proteins. The biologic effects of somatostatin are probably mediated by a family of G protein-coupled receptors that are expressed in a tissue-specific manner. SSTR4 is a member of the superfamily of receptors having seven transmembrane segments and is expressed in highest levels in fetal and adult brain and lung. View All» UniProt Summary: FUNCTION: SwissProt: P31391 # Receptor for somatostatin-14. The activity of this receptor is mediated by G proteins which inhibits adenylyl cyclase. It is functionally coupled not only to inhibition of adenylate cyclase, but also to activation of both arachidonate release and mitogen-activated protein (MAP) kinase cascade. Mediates antiproliferative action of somatostatin in tumor cells.SIZE: 388 amino acids; 41895 Da SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein.TISSUE SPECIFICITY: Specifically expressed in fetal and adult brain, lung tissue, stomach, and in lesser quantities in the kidney, pituitary and adrenals.SIMILARITY: SwissProt: P31391 ## Belongs to the G-protein coupled receptor 1 family. View All» Brand Family: Chemicon View All» Product Name: Anti-Somatostatin Receptor Type 4 Antibody | AB9582-200UL View All» Concentration: 0.75 mg/mL (after reconstitution) View All» Antibody Type: Polyclonal Antibody View All» Qty/Pk: 200 µL View All» Format: Affinity Purified View All» Host: Rabbit View All»
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